dc.creator | Martín López, María | es |
dc.creator | González Muñoz, Elena | es |
dc.creator | Gómez-González, Emilio | es |
dc.creator | Sánchez Pernaute, Rosario | es |
dc.creator | Márquez Rivas, Javier | es |
dc.creator | Fernández Muñoz, Beatriz | es |
dc.date.accessioned | 2022-09-19T16:11:26Z | |
dc.date.available | 2022-09-19T16:11:26Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Martín López, M., González Muñoz, E., Gómez González, E., Sánchez Pernaute, R., Márquez Rivas, J. y Fernández Muñoz, B. (2021). Modeling chronic cervical spinal cord injury in aged rats for cell therapy studies. Journal of Clinical Neuroscience, 94, 76-85. | |
dc.identifier.issn | 0967-5868 | es |
dc.identifier.uri | https://hdl.handle.net/11441/137212 | |
dc.description.abstract | With an expanding elderly population, an increasing number of older adults will experience spinal cord
injury (SCI) and might be candidates for cell-based therapies, yet there is a paucity of research in this age
group. The objective of the present study was to analyze how aged rats tolerate behavioral testing, sur-
gical procedures, post-operative complications, intra-spinal cell transplantation and immunosuppres-
sion, and to examine the effectiveness of human iPSC-derived Neural Progenitor Cells (IMR90-hiPSC-
NPCs) in a model of SCI. We performed behavioral tests in rats before and after inducing cervical
hemi-contusions at C4 level with a fourth-generation Ohio State University Injury Device. Four weeks
later, we injected IMR90-hiPSC-NPCs in animals that were immunosuppressed by daily cyclosporine
injection. Four weeks after injection we analyzed locomotor behavior and mortality, and histologically
assessed the survival of transplanted human NPCs. As rats aged, their success at completing behavioral
tests decreased. In addition, we observed high mortality rates during behavioral training (41.2%), after
cervical injury (63.2%) and after cell injection (50%). Histological analysis revealed that injected cells sur-
vived and remained at and around the grafted site and did not cause tumors. No locomotor improvement
was observed in animals four weeks after IMR90-hiPSC-NPC transplantation. Our results show that
elderly rats are highly vulnerable to interventions, and thus large groups of animals must be initially
established to study the potential efficacy of cell-based therapies in age-related chronic myelopathies. | es |
dc.description.sponsorship | Consejería de Transformación Económica, Industria, Conocimiento y Universidades de la Junta de Andalucía RyC 2014-15410 | es |
dc.format | application/pdf | es |
dc.format.extent | 10 p. | es |
dc.language.iso | eng | es |
dc.publisher | Churchill Livingstone | es |
dc.relation.ispartof | Journal of Clinical Neuroscience, 94, 76-85. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | SCI | es |
dc.subject | Myelopathy | es |
dc.subject | Pluripotent stem cells | es |
dc.subject | iPSCs | es |
dc.subject | NPCs | es |
dc.subject | Elderly | es |
dc.subject | Advanced therapies | es |
dc.title | Modeling chronic cervical spinal cord injury in aged rats for cell therapy studies | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Física Aplicada III | es |
dc.relation.projectID | RyC 2014-15410 | es |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0967586821004987 | es |
dc.identifier.doi | 10.1016/j.jocn.2021.09.042 | es |
dc.contributor.group | Universidad de Sevilla. TEP-203: Física Interdisciplinar Fundamentos y aplicaciones | es |
dc.journaltitle | Journal of Clinical Neuroscience | es |
dc.publication.volumen | 94 | es |
dc.publication.initialPage | 76 | es |
dc.publication.endPage | 85 | es |
dc.contributor.funder | Consejería de Salud y Familias de Andalucía a la Red Andaluza de Diseño y Traslación de Terapias Avanzadas y fondos privados. | es |