Artículo
Modeling chronic cervical spinal cord injury in aged rats for cell therapy studies
Autor/es | Martín López, María
González Muñoz, Elena Gómez-González, Emilio Sánchez Pernaute, Rosario Márquez Rivas, Javier Fernández Muñoz, Beatriz |
Departamento | Universidad de Sevilla. Departamento de Física Aplicada III |
Fecha de publicación | 2021 |
Fecha de depósito | 2022-09-19 |
Publicado en |
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Resumen | With an expanding elderly population, an increasing number of older adults will experience spinal cord
injury (SCI) and might be candidates for cell-based therapies, yet there is a paucity of research in this age
group. ... With an expanding elderly population, an increasing number of older adults will experience spinal cord injury (SCI) and might be candidates for cell-based therapies, yet there is a paucity of research in this age group. The objective of the present study was to analyze how aged rats tolerate behavioral testing, sur- gical procedures, post-operative complications, intra-spinal cell transplantation and immunosuppres- sion, and to examine the effectiveness of human iPSC-derived Neural Progenitor Cells (IMR90-hiPSC- NPCs) in a model of SCI. We performed behavioral tests in rats before and after inducing cervical hemi-contusions at C4 level with a fourth-generation Ohio State University Injury Device. Four weeks later, we injected IMR90-hiPSC-NPCs in animals that were immunosuppressed by daily cyclosporine injection. Four weeks after injection we analyzed locomotor behavior and mortality, and histologically assessed the survival of transplanted human NPCs. As rats aged, their success at completing behavioral tests decreased. In addition, we observed high mortality rates during behavioral training (41.2%), after cervical injury (63.2%) and after cell injection (50%). Histological analysis revealed that injected cells sur- vived and remained at and around the grafted site and did not cause tumors. No locomotor improvement was observed in animals four weeks after IMR90-hiPSC-NPC transplantation. Our results show that elderly rats are highly vulnerable to interventions, and thus large groups of animals must be initially established to study the potential efficacy of cell-based therapies in age-related chronic myelopathies. |
Agencias financiadoras | Consejería de Salud y Familias de Andalucía a la Red Andaluza de Diseño y Traslación de Terapias Avanzadas y fondos privados. |
Identificador del proyecto | RyC 2014-15410 |
Cita | Martín López, M., González Muñoz, E., Gómez González, E., Sánchez Pernaute, R., Márquez Rivas, J. y Fernández Muñoz, B. (2021). Modeling chronic cervical spinal cord injury in aged rats for cell therapy studies. Journal of Clinical Neuroscience, 94, 76-85. |
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