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dc.creatorSun, J.es
dc.creatorXu, W.es
dc.creatorHervás Morón, Manueles
dc.creatorNavarro Carruesco, José Antonioes
dc.creatorRosa Acosta, Miguel Ángel de laes
dc.creatorChitnis, Parag R.es
dc.date.accessioned2022-06-07T12:28:34Z
dc.date.available2022-06-07T12:28:34Z
dc.date.issued1999
dc.identifier.citationSun, J., Xu, W., Hervás Morón, M., Navarro Carruesco, J.A., Rosa Acosta, M.Á.d.l. y Chitnis, P.R. (1999). Oxidizing Side of the Cyanobacterial Photosystem I. Journal of Biological Chemistry, 274 (27), 19048-19054.
dc.identifier.issn0021-9258es
dc.identifier.urihttps://hdl.handle.net/11441/134158
dc.description.abstractPhotosystem I (PSI) interacts with plastocyanin or cytochrome c 6 on the luminal side. To identify sites of interaction between plastocyanin/cytochromec 6 and the PSI core, site-directed mutations were generated in the luminal J loop of the PsaB protein fromSynechocystis sp. PCC 6803. The eight mutant strains differed in their photoautotrophic growth. Western blotting with subunit-specific antibodies indicated that the mutations affected the PSI level in the thylakoid membranes. PSI proteins could not be detected in the S600R/G601C/N602I, N609K/S610C/T611I, and M614I/G615C/W616A mutant membranes. The other mutant strains contained different levels of PSI proteins. Among the mutant strains that contained PSI proteins, the H595C/L596I, Q627H/L628C/I629S, and N638C/N639S mutants showed similar levels of PSI-mediated electron transfer activity when either cytochrome c 6 or an artificial electron donor was used. In contrast, cytochromec 6 could not function as an electron donor to the W622C/A623R mutant, even though the PSI activity mediated by an artificial electron donor was detected in this mutant. Thus, the W622C/A623R mutation affected the interaction of the PSI complex with cytochrome c 6. Biotin-maleimide modification of the mutant PSI complexes indicated that His-595, Trp-622, Leu-628, Tyr-632, and Asn-638 in wild-type PsaB may be exposed on the surface of the PSI complex. The results presented here demonstrate the role of an extramembrane loop of a PSI core protein in the interaction with soluble electron donor proteins.es
dc.formatapplication/pdfes
dc.format.extent7 p.es
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofJournal of Biological Chemistry, 274 (27), 19048-19054.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleOxidizing Side of the Cyanobacterial Photosystem Ies
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.publisherversionhttps://dx.doi.org/10.1074/jbc.274.27.19048es
dc.identifier.doi10.1074/jbc.274.27.19048es
dc.journaltitleJournal of Biological Chemistryes
dc.publication.volumen274es
dc.publication.issue27es
dc.publication.initialPage19048es
dc.publication.endPage19054es

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