dc.creator | Sun, J. | es |
dc.creator | Xu, W. | es |
dc.creator | Hervás Morón, Manuel | es |
dc.creator | Navarro Carruesco, José Antonio | es |
dc.creator | Rosa Acosta, Miguel Ángel de la | es |
dc.creator | Chitnis, Parag R. | es |
dc.date.accessioned | 2022-06-07T12:28:34Z | |
dc.date.available | 2022-06-07T12:28:34Z | |
dc.date.issued | 1999 | |
dc.identifier.citation | Sun, J., Xu, W., Hervás Morón, M., Navarro Carruesco, J.A., Rosa Acosta, M.Á.d.l. y Chitnis, P.R. (1999). Oxidizing Side of the Cyanobacterial Photosystem I. Journal of Biological Chemistry, 274 (27), 19048-19054. | |
dc.identifier.issn | 0021-9258 | es |
dc.identifier.uri | https://hdl.handle.net/11441/134158 | |
dc.description.abstract | Photosystem I (PSI) interacts with plastocyanin or cytochrome c 6 on the luminal side. To identify sites of interaction between plastocyanin/cytochromec 6 and the PSI core, site-directed mutations were generated in the luminal J loop of the PsaB protein fromSynechocystis sp. PCC 6803. The eight mutant strains differed in their photoautotrophic growth. Western blotting with subunit-specific antibodies indicated that the mutations affected the PSI level in the thylakoid membranes. PSI proteins could not be detected in the S600R/G601C/N602I, N609K/S610C/T611I, and M614I/G615C/W616A mutant membranes. The other mutant strains contained different levels of PSI proteins. Among the mutant strains that contained PSI proteins, the H595C/L596I, Q627H/L628C/I629S, and N638C/N639S mutants showed similar levels of PSI-mediated electron transfer activity when either cytochrome c 6 or an artificial electron donor was used. In contrast, cytochromec 6 could not function as an electron donor to the W622C/A623R mutant, even though the PSI activity mediated by an artificial electron donor was detected in this mutant. Thus, the W622C/A623R mutation affected the interaction of the PSI complex with cytochrome c 6. Biotin-maleimide modification of the mutant PSI complexes indicated that His-595, Trp-622, Leu-628, Tyr-632, and Asn-638 in wild-type PsaB may be exposed on the surface of the PSI complex. The results presented here demonstrate the role of an extramembrane loop of a PSI core protein in the interaction with soluble electron donor proteins. | es |
dc.format | application/pdf | es |
dc.format.extent | 7 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Journal of Biological Chemistry, 274 (27), 19048-19054. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Oxidizing Side of the Cyanobacterial Photosystem I | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.publisherversion | https://dx.doi.org/10.1074/jbc.274.27.19048 | es |
dc.identifier.doi | 10.1074/jbc.274.27.19048 | es |
dc.journaltitle | Journal of Biological Chemistry | es |
dc.publication.volumen | 274 | es |
dc.publication.issue | 27 | es |
dc.publication.initialPage | 19048 | es |
dc.publication.endPage | 19054 | es |