dc.creator | Molina Heredia, Fernando Publio | es |
dc.creator | Díaz Quintana, Antonio Jesús | es |
dc.creator | Hervás Morón, Manuel | es |
dc.creator | Navarro Carruesco, José Antonio | es |
dc.creator | Rosa Acosta, Miguel Ángel de la | es |
dc.date.accessioned | 2022-06-07T12:21:52Z | |
dc.date.available | 2022-06-07T12:21:52Z | |
dc.date.issued | 1999 | |
dc.identifier.citation | Molina Heredia, F.P., Díaz Quintana, A.J., Hervás Morón, M., Navarro Carruesco, J.A. y Rosa Acosta, M.Á.d.l. (1999). Site-directed Mutagenesis of Cytochromec 6 from Anabaena Species PCC 7119. Journal of Biological Chemistry, 274 (47), 33565-33570. | |
dc.identifier.issn | 0021-9258 | es |
dc.identifier.uri | https://hdl.handle.net/11441/134156 | |
dc.description.abstract | A number of surface residues of cytochromec 6 from the cyanobacterium Anabaenasp. PCC 7119 have been modified by site-directed mutagenesis. Changes were made in six amino acids, two near the heme group (Val-25 and Lys-29) and four in the positively charged patch (Lys-62, Arg-64, Lys-66, and Asp-72). The reactivity of mutants toward the membrane-anchored complex photosystem I was analyzed by laser flash absorption spectroscopy. The experimental results indicate that cytochrome c 6 possesses two areas involved in the redox interaction with photosystem I: 1) a positively charged patch that may drive its electrostatic attractive movement toward photosystem I to form a transient complex and 2) a hydrophobic region at the edge of the heme pocket that may provide the contact surface for the transfer of electrons to P700. The isofunctionality of these two areas with those found in plastocyanin (which acts as an alternative electron carrier playing the same role as cytochromec 6) are evident. | es |
dc.format | application/pdf | es |
dc.format.extent | 6 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Journal of Biological Chemistry, 274 (47), 33565-33570. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Site-directed Mutagenesis of Cytochromec 6 from Anabaena Species PCC 7119 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.publisherversion | https://dx.doi.org/10.1074/jbc.274.47.33565 | es |
dc.identifier.doi | 10.1074/jbc.274.47.33565 | es |
dc.journaltitle | Journal of Biological Chemistry | es |
dc.publication.volumen | 274 | es |
dc.publication.issue | 47 | es |
dc.publication.initialPage | 33565 | es |
dc.publication.endPage | 33570 | es |