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dc.creatorMolina Heredia, Fernando Publioes
dc.creatorHervás Morón, Manueles
dc.creatorNavarro Carruesco, José Antonioes
dc.creatorRosa Acosta, Miguel Ángel de laes
dc.date.accessioned2022-06-02T16:15:16Z
dc.date.available2022-06-02T16:15:16Z
dc.date.issued2001
dc.identifier.citationMolina Heredia, F.P., Hervás Morón, M., Navarro Carruesco, J.A. y Rosa Acosta, M.Á.d.l. (2001). A Single Arginyl Residue in Plastocyanin and in Cytochrome c6 from the Cyanobacterium Anabaenasp. PCC 7119 Is Required for Efficient Reduction of Photosystem I. Journal of Biological Chemistry, 276 (1), 601-605.
dc.identifier.issn0021-9258es
dc.identifier.urihttps://hdl.handle.net/11441/133975
dc.description.abstractPositively charged plastocyanin from Anabaena sp. PCC 7119 was investigated by site-directed mutagenesis. The reactivity of its mutants toward photosystem I was analyzed by laser flash spectroscopy. Replacement of arginine at position 88, which is adjacent to the copper ligand His-87, by glutamine and, in particular, by glutamate makes plastocyanin reduce its availability for transferring electrons to photosystem I. Such a residue in the copper protein thus appears to be isofunctional with Arg-64 (which is close to the heme group) in cytochrome c6 from Anabaena(Molina-Heredia, F. P., Dı́az-Quintana, A., Hervás, M., Navarro, J. A., and De la Rosa, M. A. (1999)J. Biol. Chem. 274, 33565–33570) and Synechocystis (De la Cerda, B., Dı́az-Quintana, A., Navarro, J. A., Hervás, M., and De la Rosa, M. A. (1999) J. Biol. Chem. 274, 13292–13297). Other mutations concern specific residues of plastocyanin either at its positively charged east face (D49K, H57A, H57E, K58A, K58E, Y83A, and Y83F) or at its north hydrophobic pole (L12A, K33A, and K33E). Mutations altering the surface electrostatic potential distribution allow the copper protein to modulate its kinetic efficiency: the more positively charged the interaction site, the higher the rate constant. Whereas replacement of Tyr-83 by either alanine or phenylalanine has no effect on the kinetics of photosystem I reduction, Leu-12 and Lys-33 are essential for the reactivity of plastocyanin.es
dc.formatapplication/pdfes
dc.format.extent5 p.es
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofJournal of Biological Chemistry, 276 (1), 601-605.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleA Single Arginyl Residue in Plastocyanin and in Cytochrome c6 from the Cyanobacterium Anabaenasp. PCC 7119 Is Required for Efficient Reduction of Photosystem Ies
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.publisherversionhttps://dx.doi.org/10.1074/jbc.M007081200es
dc.identifier.doi10.1074/jbc.M007081200es
dc.journaltitleJournal of Biological Chemistryes
dc.publication.volumen276es
dc.publication.issue1es
dc.publication.initialPage601es
dc.publication.endPage605es

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