Artículo
Possible involvement of the inhibition of NF-kβ factor in anti_inflammatory actions that melatonin exert on mast cells
Autor/es | Maldonado y Aibar, María Dolores
Moreno-García, H. González Yanes, Carmen Calvo Gutiérrez, Juan Ramón |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología |
Fecha de publicación | 2016-01-08 |
Fecha de depósito | 2022-05-18 |
Publicado en |
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Resumen | Melatonin is a molecule endogenously produced in a wide variety of immune cells, including mast
cells (RBL-2H3). It exhibits immunomodulatory, anti-inflammatory and anti-apoptotic properties.
The physiologic mechanisms ... Melatonin is a molecule endogenously produced in a wide variety of immune cells, including mast cells (RBL-2H3). It exhibits immunomodulatory, anti-inflammatory and anti-apoptotic properties. The physiologic mechanisms underlying these activities of melatonin have not been clarified in mast cells. This work is designed to determine the anti-inflammatory effect and mechanism of action of melatonin on activated mast cells. RBL-2H3 were pre-treated with exogenous melatonin (MELx) at physiological (100nM) and pharmacological (1mM) doses for 30 min, washed and activated with PMACI (phorbol 12-myristate 13-acetate plus calcium ionophore A23187) for 2h and 12h. The data shows that pre-treatment of MELx in stimulated mast cells, significantly reduced the levels of endogenous melatonin production (MELn), TNF-alpha and IL-6. These effects are directly related with the MELx concentration used. MELx also inhibited IKK/NF-kB signal transduction pathway in stimulated mast cells. These results indicate a molecular basis for the ability of melatonin to prevent inflammation and for the treatment of allergic inflammatory diseases through the down-regulation of mast cell activation. |
Cita | Maldonado, M. D., García-Moreno, H., González-Yanes, C., & Calvo, J. R. (2016). Possible Involvement of the Inhibition of NF-κB Factor in Anti-Inflammatory Actions That Melatonin Exerts on Mast Cells. Journal of cellular biochemistry, 117(8), 1926–1933 |
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