dc.creator | Aguilera Romero, María Auxiliadora | es |
dc.creator | Sabido Bozo, Susana | es |
dc.creator | López Martín, Sergio | es |
dc.creator | Cortés Gómez, Alejandro | es |
dc.creator | Rodríguez Gallardo, Sofía | es |
dc.creator | Pérez Linero, Ana María | es |
dc.creator | Riezman, Isabelle | es |
dc.creator | Riezman, Howard | es |
dc.creator | Muñiz Guinea, Manuel | es |
dc.date.accessioned | 2022-05-16T10:40:52Z | |
dc.date.available | 2022-05-16T10:40:52Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Aguilera Romero, M.A., Sabido Bozo, S., López Martín, S., Cortés Gómez, A., Rodríguez Gallardo, S., Pérez Linero, A.M.,...,Muñiz Guinea, M. (2021). Determination of the lipid composition of the GPI anchor. PLOS ONE, 16 (8), e0256184. | |
dc.identifier.issn | 1932-6203 | es |
dc.identifier.uri | https://hdl.handle.net/11441/133328 | |
dc.description.abstract | In eukaryotic cells, a subset of cell surface proteins is attached by the glycolipid glycosylphosphatidylinositol (GPI) to the external leaflet of the plasma membrane where they play important roles as enzymes, receptors, or adhesion molecules. Here we present a protocol for purification and mass spectrometry analysis of the lipid moiety of individual GPI-anchored proteins (GPI-APs) in yeast. The method involves the expression of a specific GPI-AP tagged with GFP, solubilization, immunoprecipitation, separation by electrophoresis, blotting onto PVDF, release and extraction of the GPI-lipid moiety and analysis by mass spectrometry. By using this protocol, we could determine the precise GPI-lipid structure of the GPI-AP Gas1-GFP in a modified yeast strain. This protocol can be used to identify the lipid composition of the GPI anchor of distinct GPI-APs from yeast to mammals and can be adapted to determine other types of protein lipidation. | es |
dc.description.sponsorship | FEDER/Ministerio de Ciencia, Innovación y Universidades BFU2017- 89700-P | es |
dc.description.sponsorship | Universidad de Sevilla VIPPIT-2020-I.5 | es |
dc.description.sponsorship | NCCR Chemical Biology and the Swiss National Science Foundation (51NF40-185898 and 310030_184949) | es |
dc.format | application/pdf | es |
dc.format.extent | 5 p. | es |
dc.language.iso | eng | es |
dc.publisher | PLOS | es |
dc.relation.ispartof | PLOS ONE, 16 (8), e0256184. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Determination of the lipid composition of the GPI anchor | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.projectID | BFU2017-89700-P | es |
dc.relation.projectID | VIPPIT-2020-I.5 | es |
dc.relation.projectID | 51NF40-185898 | es |
dc.relation.projectID | 310030_184949 | es |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0256184 | es |
dc.identifier.doi | 10.1371/journal.pone.0256184 | es |
dc.journaltitle | PLOS ONE | es |
dc.publication.volumen | 16 | es |
dc.publication.issue | 8 | es |
dc.publication.initialPage | e0256184 | es |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (MICINN). España | es |
dc.contributor.funder | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) | es |
dc.contributor.funder | Universidad de Sevilla | es |
dc.contributor.funder | Swiss National Science Foundation (SNFS) | es |