dc.creator | Garriga Román, Carles | es |
dc.creator | Vázquez Cueto, Carmen María | es |
dc.creator | Ruiz Gutiérrez, Valentina | es |
dc.creator | Planas Rosselló, Joana | es |
dc.date.accessioned | 2022-04-19T09:51:47Z | |
dc.date.available | 2022-04-19T09:51:47Z | |
dc.date.issued | 2002 | |
dc.identifier.citation | Garriga Román, C., Vázquez Cueto, C.M., Ruiz Gutiérrez, V. y Planas Rosselló, J. (2002). Regional differences in transport, lipid composition, and fluidity of apical membranes of small intestine of chicken. Poultry Science, 81 (4), 537-545. | |
dc.identifier.issn | 0032-5791 | es |
dc.identifier.issn | 1525-3171 | es |
dc.identifier.uri | https://hdl.handle.net/11441/132146 | |
dc.description.abstract | Na+-dependent D-glucose transport was studied in brush-border membrane vesicles from duodenum, jejunum, and ileum of 5- to 6-wk-old chickens. Regional differences were found, and both initial rates and accumulation ratio of D-glucose were higher in the proximal part of the small intestine than in the ileum. To establish the mechanism(s) underlying these differences we have studied the density of Na+-dependent D-glucose cotransporter (SGLT1) as well as lipid composition and fluidity. Phlorizin-specific binding and Western blot analysis indicated a decrease in the amount of SGLT1 in the ileum when compared to the duodenum and jejunum. The distal part of the small intestine also showed a decrease in free cholesterol content and saturated-to-unsaturated fatty acid ratio together with an increase in lipid content and phosphatidylcholine-to-sphingomyelin ratio. These results were associated with a decrease in the diphenylhextriene fluorescence polarization found in brush-border membranes of the ileum. We can conclude that the decrease in the apical D-glucose transport found in the ileum is primarily due to a reduction in the amount of SGLT1 present in the brush-border membrane rather than the differences in the lipid composition and fluidity. | es |
dc.description.sponsorship | Ministerio de Educación y Cultura de España. PB96/1255 | es |
dc.description.sponsorship | Generalitat de Catalunya.1999-SGR-00271 | es |
dc.format | application/pdf | es |
dc.format.extent | 9 p. | es |
dc.language.iso | eng | es |
dc.publisher | Poultry Science Association | es |
dc.relation.ispartof | Poultry Science, 81 (4), 537-545. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | brush-border membrane vesicles | es |
dc.subject | sodium-glucose transporter isoform 1 | es |
dc.subject | phospholipid | es |
dc.subject | fatty acid | es |
dc.subject | cholesterol | es |
dc.title | Regional differences in transport, lipid composition, and fluidity of apical membranes of small intestine of chicken | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.projectID | PB96/1255 | es |
dc.relation.projectID | 1999-SGR-00271 | es |
dc.relation.publisherversion | https://doi.org/10.1093/ps/81.4.537 | es |
dc.identifier.doi | 10.1093/ps/81.4.537 | es |
dc.journaltitle | Poultry Science | es |
dc.publication.volumen | 81 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 537 | es |
dc.publication.endPage | 545 | es |
dc.identifier.sisius | 6688989 | es |
dc.contributor.funder | Ministerio de Educación y Cultura (MEC). España | es |
dc.contributor.funder | Generalitat de Catalunya | es |