Presentation
Effects of electrical fields on neuroblastoma (N2A) cell differentiation: preliminary results
Author/s | Martín Fernández, Daniel
Pérez García, Pablo Martín Rubio, María Esther Daza Navarro, María Paula Serrano Viseas, Juan Alfonso Huertas Sánchez, Gloria Yúfera García, Alberto |
Department | Universidad de Sevilla. Departamento de Biología Celular Universidad de Sevilla. Departamento de Tecnología Electrónica Universidad de Sevilla. Departamento de Electrónica y Electromagnetismo |
Publication Date | 2021 |
Deposit Date | 2022-04-01 |
Published in |
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ISBN/ISSN | 978-989-758-490-9 2184-4305 |
Abstract | This work describes Electrical Stimulations (ES) assays on stem cells. The neuroblastoma (N2A) cell linage was submitted to several electrical fields to enable and enhance its differentiation toward neurons. Both Direct ... This work describes Electrical Stimulations (ES) assays on stem cells. The neuroblastoma (N2A) cell linage was submitted to several electrical fields to enable and enhance its differentiation toward neurons. Both Direct Current (DC) and Alternated Current (AC) time dependent electric field protocols were applied to N2A cell culture under differentiation conditions, obtaining different responses. Control and electrically excited samples’ number of differentiated cells and neurite lengths were measure after differentiation. Results showed that DC fields have a strong influence on N2A differentiation since the percentage of differentiated cells and the neurites lengths were the highest. In addition, a significant alignment of neurites measured with the applied electrical field has been detected, which demonstrates the high sensitivity of differentiation processes to electrical field polarity. |
Funding agencies | Junta de Andalucía Ministerio de Ciencia, Innovación y Universidades (MICINN). España |
Project ID. | P18-FR-2308
RTI2018-093512-B-C21 |
Citation | Martín Fernández, D., Pérez García, P., Martín Rubio, M.E., Daza Navarro, M.P., Serrano Viseas, J.A., Huertas Sánchez, G. y Yúfera García, A. (2021). Effects of electrical fields on neuroblastoma (N2A) cell differentiation: preliminary results. En Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologiess - BIODEVICES (152-159), Scitepress. |
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