dc.creator | Pacios, Olga | es |
dc.creator | Fernández García, Laura | es |
dc.creator | Bleriot, Inés | es |
dc.creator | Blasco, Lucía | es |
dc.creator | Pascual Hernández, Álvaro | es |
dc.creator | Tomás, María | es |
dc.date.accessioned | 2022-03-18T12:35:17Z | |
dc.date.available | 2022-03-18T12:35:17Z | |
dc.date.issued | 2021-12-31 | |
dc.identifier.citation | Pacios, O., Fernández García, L., Bleriot, I., Blasco, L., Pascual Hernández, Á. y Tomás, M. (2021). Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13. Viruses, 14 (1) | |
dc.identifier.issn | 1999-4915 | es |
dc.identifier.uri | https://hdl.handle.net/11441/131012 | |
dc.description.abstract | Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immuno compromised patients. Here, we annotated and compared the genomes of two lytic phages that infect
clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically char acterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected
cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that
vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylo genetic study performed with other Tevenvirinae phages showed a close common ancestor. How ever, there were 21 coding sequences which differed. Interestingly, the main differences were that
vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nu cleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading
to different three-dimensional protein predictions. Both phages differed significantly in their host
range. These viruses may be useful in the development of alternative therapies to antibiotics or as
a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant
(MDR) pathogens. | es |
dc.description.sponsorship | Instituto de Salud Carlos III: PI19/00878 | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Viruses, 14 (1) | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Lytic phages | es |
dc.subject | Klebsiella pneumoniae | es |
dc.subject | Genomic annotation | es |
dc.subject | Homing endonucleases | es |
dc.subject | L-shaped tail fiber | es |
dc.title | Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | https://www.mdpi.com/2077-0383/11/1/84 | es |
dc.identifier.doi | 10.3390/v14010006 | es |
dc.journaltitle | Viruses | es |
dc.publication.volumen | 14 | es |
dc.publication.issue | 1 | es |