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dc.creatorPacios, Olgaes
dc.creatorFernández García, Lauraes
dc.creatorBleriot, Inéses
dc.creatorBlasco, Lucíaes
dc.creatorPascual Hernández, Álvaroes
dc.creatorTomás, Maríaes
dc.date.accessioned2022-03-18T12:35:17Z
dc.date.available2022-03-18T12:35:17Z
dc.date.issued2021-12-31
dc.identifier.citationPacios, O., Fernández García, L., Bleriot, I., Blasco, L., Pascual Hernández, Á. y Tomás, M. (2021). Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13. Viruses, 14 (1)
dc.identifier.issn1999-4915es
dc.identifier.urihttps://hdl.handle.net/11441/131012
dc.description.abstractKlebsiella pneumoniae is a human pathogen that worsens the prognosis of many immuno compromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically char acterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylo genetic study performed with other Tevenvirinae phages showed a close common ancestor. How ever, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nu cleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens.es
dc.description.sponsorshipInstituto de Salud Carlos III: PI19/00878es
dc.formatapplication/pdfes
dc.format.extent16 p.es
dc.language.isoenges
dc.publisherMDPIes
dc.relation.ispartofViruses, 14 (1)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLytic phageses
dc.subjectKlebsiella pneumoniaees
dc.subjectGenomic annotationes
dc.subjectHoming endonucleaseses
dc.subjectL-shaped tail fiberes
dc.titlePhenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13es
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.publisherversionhttps://www.mdpi.com/2077-0383/11/1/84es
dc.identifier.doi10.3390/v14010006es
dc.journaltitleViruseses
dc.publication.volumen14es
dc.publication.issue1es

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Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional