Mostrar el registro sencillo del ítem

Artículo

dc.creatorVan Den Heuvel, Robert H.H.es
dc.creatorFerrari, Davidees
dc.creatorBossi, Roberto T.es
dc.creatorRavasio, Sergioes
dc.creatorCurti, Brunoes
dc.creatorVanoni, Maria A.es
dc.creatorFlorencio Bellido, Francisco Javieres
dc.creatorMattevi, Andreaes
dc.date.accessioned2022-03-16T09:55:45Z
dc.date.available2022-03-16T09:55:45Z
dc.date.issued2002
dc.identifier.citationVan Den Heuvel, R.H.H., Ferrari, D., Bossi, R.T., Ravasio, S., Curti, B., Vanoni, M.A.,...,Mattevi, A. (2002). Structural studies on the synchronization of catalytic centers in glutamate synthase. Journal Of Biological Chemistry, 277 (27), 24579-24583.
dc.identifier.issn1083-351Xes
dc.identifier.issn0021-9258es
dc.identifier.urihttps://hdl.handle.net/11441/130860
dc.description.abstractThe complex iron-sulfur flavoprotein glutamate synthase (GltS) plays a prominent role in ammonia assimilation in bacteria, yeasts, and plants. GltS catalyzes the formation of two molecules of L-glutamate from 2-oxoglutarate and L-glutamine via intramolecular channeling of ammonia. GltS has the impressive ability of synchronizing its distinct catalytic centers to avoid wasteful consumption of L-glutamine. We have determined the crystal structure of the ferredoxin-dependent GltS in several ligation and redox states. The structures reveal the crucial elements in the synchronization between the glutaminase site and the 2-iminoglutarate reduction site. The structural data combined with the catalytic properties of GltS indicate that binding of ferredoxin and 2-oxoglutarate to the FMN-binding domain of GltS induce a conformational change in the loop connecting the two catalytic centers. The rearrangement induces a shift in the catalytic elements of the amidotransferase domain, such that it becomes activated. This machinery, over a distance of more than 30 Å, controls the ability of the enzyme to bind and hydrolyze the ammonia-donating substrate L-glutaminees
dc.formatapplication/pdfes
dc.format.extent8 p.es
dc.language.isoenges
dc.publisherAmerican Society for Biochemistry and Molecular Biologyes
dc.relation.ispartofJournal Of Biological Chemistry, 277 (27), 24579-24583.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleStructural studies on the synchronization of catalytic centers in glutamate synthasees
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.publisherversionhttps://doi.org/10.1074/jbc.M202541200es
dc.identifier.doi10.1074/jbc.M202541200es
dc.journaltitleJournal Of Biological Chemistryes
dc.publication.volumen277es
dc.publication.issue27es
dc.publication.initialPage24579es
dc.publication.endPage24583es
dc.identifier.sisius6699377es

FicherosTamañoFormatoVerDescripción
PIIS0021925819666378.pdf323.6KbIcon   [PDF] Ver/Abrir  

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional