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dc.creatorKobayashi, Masakies
dc.creatorRodríguez, Rocíoes
dc.creatorLara Coronado, Catalinaes
dc.creatorOmata, Tatsuoes
dc.date.accessioned2022-02-04T15:51:28Z
dc.date.available2022-02-04T15:51:28Z
dc.date.issued1997
dc.identifier.citationKobayashi, M., Rodríguez, R., Lara Coronado, C. y Omata, T. (1997). Involvement of the C-terminal domain of an ATP-binding subunit in the regulation of the ABC-type nitrate/nitrite transporter of the cyanobacterium Synechococcus sp. Strain PCC 7942. Journal of Biological Chemistry, 272 (43), 27197-27201.
dc.identifier.issn0021-9258es
dc.identifier.issn1083-351Xes
dc.identifier.urihttps://hdl.handle.net/11441/129668
dc.description.abstractIn Synechococcus sp. strain PCC 7942, an ATP-binding cassette transporter encoded by the genes nrtA, nrtB, nrtC, and nrtD mediates active transport of nitrate and nitrite, which is inhibited by ammonium, a preferred source of nitrogen for the cyanobacterium. One of the ATP-binding subunits of the transporter, NrtC, has a distinct C-terminal domain of 380 amino acid residues. A mutant NC2, constructed by removal of this domain using genetic engineering techniques, assimilated low concentrations of nitrate and nitrite and accumulated nitrate intracellularly, showing that the domain is not essential for the transporter activities. Assimilation of low concentrations of nitrite was only partially inhibited by ammonium in NC2 but was completely inhibited in the wild-type cells. Cells of NC2 and its derivative (nitrate reductase-less strain NC4) carrying the truncated NrtC but not the cells with the wild-type NrtC accumulated nitrate intracellularly in the presence of ammonium in medium. These findings indicated that the C-terminal domain of NrtC is involved in the ammonium-promoted inhibition of the nitrate/nitrite transporter. In the presence of ammonium, NC2 could not assimilate nitrate despite its ability to accumulate nitrate intracellularly, which suggested that reduction of intracellular nitrate by nitrate reductase is also subject to inhibition by ammonium.es
dc.description.sponsorshipMinistry of Education, Science and Culture, Japan 09274101, 09274103es
dc.formatapplication/pdfes
dc.format.extent5 p.es
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofJournal of Biological Chemistry, 272 (43), 27197-27201.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleInvolvement of the C-terminal domain of an ATP-binding subunit in the regulation of the ABC-type nitrate/nitrite transporter of the cyanobacterium Synechococcus sp. Strain PCC 7942es
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectID09274101es
dc.relation.projectID09274103es
dc.relation.projectIDPB94-1191-CO2es
dc.relation.publisherversionhttps://doi.org/10.1074/jbc.272.43.27197es
dc.identifier.doi10.1074/jbc.272.43.27197es
dc.journaltitleJournal of Biological Chemistryes
dc.publication.volumen272es
dc.publication.issue43es
dc.publication.initialPage27197es
dc.publication.endPage27201es
dc.contributor.funderDirección General de Investigación Científica y Técnica (DGICYT). España PB94-1191-CO2es

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