A microfluidic liquid phase microextraction method for drugs and parabens monitoring in human urine

Abstract

Frequent consumption of pharmaceuticals and personal care products (PPCPs) have emerged as a current problem that highlights the pressing need for new multi-residue analytical methods that allow their simultaneous determination to assess their overall effect on human health. In this regard and for the first time, a versatile microfluidic based- liquid phase microextraction (LPME) method was developed for simultaneous monitoring of ten compounds from six different classes: amoxicillin, sulfadiazine, sulfamerazine, tiamphenicol, ethyl 4-hydroxybenzoate, flumequine, propyl 4-hydroxybenzoate, 5-hydroxydiclofenac, 3-hydroxydiclofenac and diclofenac. The microfluidic device was combined with a HPLC-UV system for the separation and determination of the model analytes in the sample. Optimal conditions were reached using 2-nitrophenyl octyl ether as supported liquid membrane, pH 3.5 as donor phase, pH 11.5 as acceptor phase, 0.5 µL min−1 as donor flow rate and 1 µL min−1 as acceptor flow rate. Under optimal method conditions, the extraction efficiency was between 85 and 100% for most compounds after 10 min extraction, and it was successfully applied in non-diluted human urine, with recoveries between 70 and 100% for all analytes except for sulfamerazine (52% recovery). In addition, the extraction of metabolites (3-hydroxydiclofenac and 5-hydroxydiclofenac) was also demonstrated in microfluidic systems with recoveries between 71 and 100% in human urine. The proposed method allowed consecutive extraction and only requires 5 µL of organic solvent and less than 15 µL of sample volume.