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dc.creatorRodríguez Martínez, José Manueles
dc.creatorLópez Cerero, Lorenaes
dc.creatorGarcía-Duque, Anaes
dc.creatorRodríguez-Baño, Jesúses
dc.creatorPascual Hernández, Álvaroes
dc.date.accessioned2021-11-30T11:15:06Z
dc.date.available2021-11-30T11:15:06Z
dc.date.issued2021-11-17
dc.identifier.citationRodríguez Martínez, J.M., López Cerero, L., García-Duque, A., Rodríguez-Baño, J. y Pascual Hernández, Á. (2021). Interplay between IncF plasmids and topoisomerase mutations conferring quinolone resistance in the Escherichia coli ST131 clone: stability and resistance evolution. European Journal of Clinical Microbiology & Infectious Diseases. https://doi.org/10.1007/s10096-021-04358-4.
dc.identifier.issn0934-9723es
dc.identifier.issn1435-4373 (electrónico)es
dc.identifier.urihttps://hdl.handle.net/11441/127836
dc.description.abstractThe Escherichia coli ST131 H30-Rx subclone vehicles CTX-M-15 plasmids and mutations in gyrA and parC conferring multidrug resistance successfully in the clinical setting. The aim of this study was (1) to investigate the relationship of specific topoisomerase mutations on the stability of IncF (CTX-M producing) plasmids using isogenic E. coli mutants and (2) to investigate the impact of the IncF-type plasmids present in the E. coli clone ST131 on the evolution of quinolone resistance. E. coli ATCC 25922 (background strain) and derived mutants encoding specific QRDR substitutions were used. Also, NGS-characterized IncFIA and IncFIB plasmids (encoding CTX-M genes) were included. Plasmid stability was evaluated by sequential dilutions into Luria broth medium without antibiotics for 7 days. Mutant frequency to ciprofloxacin was also evaluated. Moderate differences in the IncF plasmids stability were observed among E. coli ATCC 25922 and isogenic mutants. Under our experimental conditions, the fluctuation of bacteria harboring plasmids was less than 0.5-log(10) in all cases. In the mutant frequency tests, it was observed that the presence of these IncF plasmids increased this value significantly (10–1000-fold). Quinolone resistance substitutions in gyrA or parC genes, frequently found associated with E. coli clone ST131, do not modify the stability of ST131-associated IncFIA and IncFIB plasmids under in vitro conditions. IncF-type plasmids present in E. coli clone ST131 facilitate the selection of resistance to quinolones. These results are consistent with the clinical scenario in which the combination of resistance to quinolones and beta-lactams is highly frequent in the E. coli clone ST131.es
dc.description.sponsorshipPlan Nacional de I + D + i 2013-2016 y el Instituto de Salud Carlos III, AC16 / 00072es
dc.description.sponsorshipProgramación Conjunta sobre resistencia a los antimicrobianos, JPIAMR con un enfoque de Una sola salud; y Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, español Red de Investigación en Enfermedades Infecciosas , REIPI; RD16 / 0016/0001 y REIPI RD16 / 0016/0009es
dc.formatapplication/pdfes
dc.format.extent7 p.es
dc.language.isoenges
dc.publisherSpringeres
dc.relation.ispartofEuropean Journal of Clinical Microbiology & Infectious Diseases.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectE. coli ST131 clonees
dc.subjectQuinoloneses
dc.subjectBeta-lactamses
dc.subjectResistancees
dc.subjectIncF plasmidses
dc.titleInterplay between IncF plasmids and topoisomerase mutations conferring quinolone resistance in the Escherichia coli ST131 clone: stability and resistance evolutiones
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.projectIDAC16 / 00072es
dc.relation.projectIDRD16 / 0016/0001es
dc.relation.projectIDREIPI RD16 / 0016/0009es
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10096-021-04358-4es
dc.identifier.doi10.1007/s10096-021-04358-4es
dc.journaltitleEuropean Journal of Clinical Microbiology & Infectious Diseaseses

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