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Mostrando ítems 1-10 de 11
Artículo
The DNA damage response acts as a safeguardagainst harmful DNA–RNA hybrids ofdifferent origins
(EMBO Press, 2019-07-24)
Despite playing physiological roles in specific situations, DNA–RNA hybrids threat genome integrity. To investigate how cells do counteract spontaneous DNA–RNA hybrids, here we screen an siRNA library covering 240 human ...
Artículo
Roles of human POLD1 and POLD3 in genome stability
(Nature, 2016)
DNA replication is essential for cellular proliferation. If improperly controlled it can constitute a major source of genome instability, frequently associated with cancer and aging. POLD1 is the catalytic subunit and POLD3 ...
Artículo
BRCA2 promotes DNA-RNA hybrid resolution by DDX5 helicase at DNA breaks to facilitate their repair
(Wiley-Blackwell, 2021)
The BRCA2 tumor suppressor is a DNA double-strand break (DSB) repair factor essential for maintaining genome integrity. BRCA2-deficient cells spontaneously accumulate DNA-RNA hybrids, a known source of genome instability. ...
Artículo
UAP56/DDX39B is a major cotranscriptional RNA–DNA helicase that unwinds harmful R loops genome-wide
(Cold Spring Harbor Laboratory Press, 2020)
Nonscheduled R loops represent a major source of DNA damage and replication stress. Cells have different ways to prevent R-loop accumulation. One mechanism relies on the conserved THO complex in association with cotranscriptional ...
Artículo
The Fanconi Anemia Pathway Protects Genome Integrity from R-loops
(Public Library of Science, 2015)
Co-transcriptional RNA-DNA hybrids (R loops) cause genome instability. To prevent harmful R loop accumulation, cells have evolved specific eukaryotic factors, one being the BRCA2 double-strand break repair protein. As BRCA2 ...
Artículo
Topoisomerase 1-dependent R-loop deficiency drives accelerated replication and genomic instability
(Elsevier, 2022)
DNA replication is a complex process tightly regulated to ensure faithful genome duplication, and its perturbation leads to DNA damage and genomic instability. Replication stress is commonly associated with slow and stalled ...
Artículo
The SWI/SNF chromatin remodeling complex helps resolve R-loop-mediated transcription–replication conflicts
(Springer Nature, 2021)
ATP-dependent chromatin remodelers are commonly mutated in human cancer. Mammalian SWI/SNF complexes comprise three conserved multisubunit chromatin remodelers (cBAF, ncBAF and PBAF) that share the BRG1 (also known as ...
Artículo
The yeast and human FACT chromatin-reorganizing complexes resolve R-loop-mediated transcription-replication conflicts
(Cold Spring Harbor Laboratory Press, 2014)
FACT (facilitates chromatin transcription) is a chromatin-reorganizing complex that swaps nucleosomes around the RNA polymerase during transcription elongation and has a role in replication that is not fully understood ...
Artículo
Polycomb RING1A- and RING1B-dependent histone H2A monoubiquitylation at pericentromeric regions promotes S-phase progression
(Company of Biologists Ltd, 2015)
The functions of polycomb products extend beyond their well-known activity as transcriptional regulators to include genome duplication processes. Polycomb activities during DNA replication and DNA damage repair are unclear, ...
Artículo
The human nucleoporin Tpr protects cells from RNA-mediated replication stress
(Springer Nature, 2021)
Although human nucleoporin Tpr is frequently deregulated in cancer, its roles are poorly understood. Here we show that Tpr depletion generates transcription-dependent replication stress, DNA breaks, and genomic instability. ...