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Mostrando ítems 1-10 de 108
Artículo
The DNA damage response acts as a safeguardagainst harmful DNA–RNA hybrids ofdifferent origins
(EMBO Press, 2019-07-24)
Despite playing physiological roles in specific situations, DNA–RNA hybrids threat genome integrity. To investigate how cells do counteract spontaneous DNA–RNA hybrids, here we screen an siRNA library covering 240 human ...
Artículo
Artículo
What causes an RNA-DNA hybrid to compromise genome integrity?
(Elsevier, 2019)
Transcription is a source of genome instability that stimulates mutation and recombination. Part of the damage produced by transcription is mediated by R-loops, non-B DNA structures that normally form by the re-annealing ...
Artículo
Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair
(Public Library of Science, 2009)
RNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for transcription resumption. One important type of hindrance consists of DNA lesions, which are removed ...
Artículo
Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation
(American Society for Biochemistry and Molecular Biology Inc., 2003)
THO/TREX is a conserved eukaryotic complex formed by the core THO complex plus proteins involved in mRNA metabolism and export such as Sub2 and Yra1. Mutations in any of the THO/TREX structural genes cause pleiotropic ...
Tesis Doctoral
Papel del RNA en expresión genética y recombinación en Saccharomyces cerevisiae
(2004)
La información necesaria para la supervivencia celular se encuentra codificada en el genoma. Cualquier proceso que conlleve la alteración de dicha información puede provocar la incapacidad del organismo para sobrevivir, ...
Artículo
Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?
(Wiley-Blackwell, 2023)
Double-strand breaks (DSBs) are the most harmful DNA lesions, with a strong impact on cell proliferation and genome integrity. Depending on cell cycle stage, DSBs are preferentially repaired by non-homologous end joining ...
Artículo
The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants
(American Society for Microbiology, 2009)
Cotranscriptional R-loops are formed in yeast mutants of the THO complex, which functions at the interface between transcription and mRNA export. Despite the relevance of R-loops in transcription-associated recombination, the ...
Artículo
The chromatin network helps prevent cancer-associated mutagenesis at transcription-replication conflicts
(Nature Research, 2023)
Genome instability is a feature of cancer cells, transcription being an important source of DNA damage. This is in large part associated with R-loops, which hamper replication, especially at head-on transcription-replication ...
Tesis Doctoral
Papel de factores de la transcripción y la replicación del ADN en el origen de la inestabilidad genómica.
(2017-09-25)
Mantener la integridad del genoma no es tarea fácil para las células. El ADN sufre constantes ataques de agentes químicos y/o físicos de origen exógeno y endógeno, pero no sólo eso, los propios procesos celulares que emplean ...