Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation
|Author/s||García Rondón, Ana
Jimeno González, Sonia
García Rubio, María Luisa
Aguilera López, Andrés
|Department||Universidad de Sevilla. Departamento de Genética|
|Abstract||THO/TREX is a conserved eukaryotic complex formed by the core THO complex plus proteins involved in mRNA metabolism and export such as Sub2 and Yra1. Mutations in any of the THO/TREX structural genes cause pleiotropic ...
THO/TREX is a conserved eukaryotic complex formed by the core THO complex plus proteins involved in mRNA metabolism and export such as Sub2 and Yra1. Mutations in any of the THO/TREX structural genes cause pleiotropic phenotypes such as transcription impairment, increased transcription-associated recombination, and mRNA export defects. To assay the relevance of THO/TREX complex in transcription, we performed in vitro transcription elongation assays in mutant cell extracts using supercoiled DNA templates containing two G-less cassettes. With these assays, we demonstrate that hpr1Δ, tho2Δ, and mft1Δ mutants of the THO complex and sub2 mutants show significant reductions in the efficiency of transcription elongation. The mRNA expression defect of hpr1Δ mutants was not due to an increase in mRNA decay, as determined by mRNA half-life measurements and mRNA time course accumulation experiments in the absence of Rrp6p exoribonuclease. This work demonstrates that THO and Sub2 are required for efficient transcription elongation, providing further evidence for the coupling between transcription and mRNA metabolism and export.
|Funding agencies||Ministerio de Ciencia y Tecnología (MCYT). España
Human Frontier Science Program (HFSP)
|Citation||García Rondón, A., Jimeno González, S., García Rubio, M.L. y Aguilera López, A. (2003). Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation. Journal of Biological Chemistry, 278 (40), 39037-39043.|