dc.creator | Riedel, Rodrigo | es |
dc.creator | Pérez Pérez, Antonio | es |
dc.creator | Carmona Fernández, Antonio | es |
dc.creator | Dueñas Díez, José Luis | es |
dc.creator | Guadix, Pilar | es |
dc.creator | Sánchez Margalet, Víctor | es |
dc.creator | Maymó, Julieta L. | |
dc.date.accessioned | 2021-05-05T17:07:31Z | |
dc.date.available | 2021-05-05T17:07:31Z | |
dc.date.issued | 2019-10-02 | |
dc.identifier.citation | Riedel, R., Pérez Pérez, A., Carmona Fernández, A., Guadix, P., Sánchez Margalet, V. y Maymó, J.L. (2019). Human amniotic membrane conditioned medium inhibits proliferation and modulates related microRNAs expression in hepatocarcinoma cell. Scientific Reports, 9 (14193) | |
dc.identifier.issn | 2045-2322 | es |
dc.identifier.uri | https://hdl.handle.net/11441/108578 | |
dc.description.abstract | The placental stem cells have called the focus of attention for their therapeutic potential to treat
diferent diseases, including cancer. There is plenty evidence about the antiproliferative, antiangiogenic
and proapoptotic properties of the amniotic membrane. Liver cancer is the ffth cause of cancer in the
world, with a poor prognosis and survival. Alternative treatments to radio- or chemotherapy have
been searched. In this work we aimed to study the antiproliferative properties of the human amniotic
membrane conditioned medium (AM-CM) in hepatocarcinoma cells. In addition, we have analyzed
the regulation of pro and antiOncomiRs expression involved in hepatocarcinoma physiology. We have
determined by 3H-thymidine incorporation assay that AM-CM inhibits DNA synthesis in HepG2 cells
after 72h of treatment. AM-CM pure or diluted at 50% and 25% also diminished HepG2 and HuH-7 cells
viability and cell number. Furthermore, AM-CM induced cell cycle arrest in G2/M. When proliferation
mechanisms were analyzed we found that AM-CM reduced the expression of both Cyclin D1 mRNA
and protein. Nuclear expression of Ki-67 was also reduced. We observed that this CM was able to
promote the expression of p53 and p21 mRNA and proteins, leading to cell growth arrest. Moreover,
AM-CM induced an increase in nuclear p21 localization, observed by immunofuorescence. As p53
levels were increased, Mdm-2 expression was downregulated. Interestingly, HepG2 and HuH-7 cells
treatment with AM-CM during 24 and 72h produced an upregulation of antiOncomiRs 15a and 210, and
a downregulation of proOncomiRs 206 and 145. We provide new evidence about the promising novel
applications of human amniotic membrane in liver cancer. | es |
dc.format | application/pdf | es |
dc.format.extent | 20 p. | es |
dc.language.iso | eng | es |
dc.relation.ispartof | Scientific Reports, 9 (14193) | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | MicroRNAs | es |
dc.subject | AM-CM | es |
dc.subject | Hepatocarcinoma cells | es |
dc.title | Human amniotic membrane conditioned medium inhibits proliferation and modulates related microRNAs expression in hepatocarcinoma cell | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Méd.y Biol.Molecular e Inmun. | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Cirugía | es |
dc.relation.publisherversion | https://doi.org/10.1038/s41598-019-50648-5 | es |
dc.identifier.doi | 10.1038/s41598-019-50648-5 | es |
dc.journaltitle | Scientific Reports | es |
dc.publication.volumen | 9 | es |
dc.publication.issue | 14193 | es |