Por motivos de mantenimiento se ha deshabilitado el inicio de sesión temporalmente. Rogamos disculpen las molestias.
Artículo
Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons
Autor/es | Carrascal Moreno, María Livia
Gorton, Ella Pardillo Díaz, Ricardo Pérez García, Patricia Gómez Oliva, Ricardo Nuñez Abades, Pedro Antonio |
Departamento | Universidad de Sevilla. Departamento de Fisiología |
Fecha de publicación | 2020-12 |
Fecha de depósito | 2021-03-03 |
Publicado en |
|
Resumen | Oxidative stress is one of the main proposed mechanisms involved in neuronal degeneration.
To evaluate the consequences of oxidative stress on motor cortex pyramidal neurons during postnatal
development, rats were ... Oxidative stress is one of the main proposed mechanisms involved in neuronal degeneration. To evaluate the consequences of oxidative stress on motor cortex pyramidal neurons during postnatal development, rats were classified into three groups: Newborn (P2–P7); infantile (P11–P15); and young adult (P20–P40). Oxidative stress was induced by 10 µM of cumene hydroperoxide (CH) application. In newborn rats, using the whole cell patch-clamp technique in brain slices, no significant modifications in membrane excitability were found. In infantile rats, the input resistance increased and rheobase decreased due to the blockage of GABAergic tonic conductance. Lipid peroxidation induced by CH resulted in a noticeable increase in protein-bound 4-hidroxynonenal in homogenates in only infantile and young adult rat slices. Interestingly, homogenates of newborn rat brain slices showed the highest capacity to respond to oxidative stress by dramatically increasing their glutathione and free thiol content. This increase correlated with a time-dependent increase in the glutathione reductase activity, suggesting a greater buffering capacity of newborn rats to resist oxidative stress. Furthermore, pre-treatment of the slices with glutathione monoethyl ester acted as a neuroprotector in pyramidal neurons of infantile rats. We conclude that during maturation, the vulnerability to oxidative stress in rat motor neurons increases with age. |
Agencias financiadoras | Ministerio de Ciencia, Innovación y Universidades (MICINN). España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Junta de Andalucía |
Identificador del proyecto | RTI2018-099908-B-C21
FEDER-UCA18-106647 |
Cita | Carrascal Moreno, M.L., Gorton, E., Pardillo Díaz, R., Pérez García, P., Gómez Oliva, R. y Nuñez Abades, P.A. (2020). Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons. Antioxidants, 9 (12), 1307. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
antioxidants-09-01307-v2.pdf | 2.696Mb | [PDF] | Ver/ | |