dc.creator | Herrera Lira, Alejandro | es |
dc.creator | Morcuende Fernández, Sara R. | es |
dc.creator | Talaverón Aguilocho, Rocío | es |
dc.creator | Benítez Temiño, Beatriz | es |
dc.creator | Pastor Loro, Ángel Manuel | es |
dc.creator | Rodríguez Matarredona, Esperanza | es |
dc.date.accessioned | 2021-02-02T11:27:49Z | |
dc.date.available | 2021-02-02T11:27:49Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Herrera Lira, A., Morcuende Fernández, S.R., Talaverón Aguilocho, R., Benítez Temiño, B., Pastor Loro, Á.M. y Rodríguez Matarredona, E. (2021). Purinergic Receptor Blockade with Suramin Increases Survival of Postnatal Neural Progenitor Cells In Vitro. International Journal of Molecular Sciences, 22 (2), 713. | |
dc.identifier.issn | 1422-0067 | es |
dc.identifier.uri | https://hdl.handle.net/11441/104439 | |
dc.description.abstract | Neural progenitor cells (NPCs) are self-renewing and multipotent cells that persist in the
postnatal and adult brain in the subventricular zone and the hippocampus. NPCs can be expanded
in vitro to be used in cell therapy. However, expansion is limited, since the survival and proliferation
of adult NPCs decrease with serial passages. Many signaling pathways control NPC survival and
renewal. Among these, purinergic receptor activation exerts differential effects on the biology of adult
NPCs depending on the cellular context. In this study, we sought to analyze the effect of a general
blockade of purinergic receptors with suramin on the proliferation and survival of NPCs isolated
from the subventricular zone of postnatal rats, which are cultured as neurospheres. Treatment of
neurospheres with suramin induced a significant increase in neurosphere diameter and in NPC
number attributed to a decrease in apoptosis. Proliferation and multipotency were not affected.
Suramin also induced an increase in the gap junction protein connexin43 and in vascular endothelial
growth factor, which might be involved in the anti-apoptotic effect. Our results offer a valuable tool
for increasing NPC survival before implantation in the lesioned brain and open the possibility of
using this drug as adjunctive therapy to NPC transplantation. | es |
dc.description.sponsorship | España Ministerio de Economía y Competitividad , grant number BFU2015-64515-P | es |
dc.description.sponsorship | España Ministerio de Ciencia, Innovación y Universidades , grant number PGC2018-094654-B-100 | es |
dc.format | application/pdf | es |
dc.format.extent | 14 | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | International Journal of Molecular Sciences, 22 (2), 713. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | purinergic signaling | es |
dc.subject | apoptosis | es |
dc.subject | neural progenitor cells | es |
dc.subject | cell therapy | es |
dc.subject | suramin | es |
dc.title | Purinergic Receptor Blockade with Suramin Increases Survival of Postnatal Neural Progenitor Cells In Vitro | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.projectID | BFU2015-64515-P | es |
dc.relation.projectID | PGC2018-094654-B-100 | es |
dc.relation.publisherversion | https://doi.org/10.3390/ijms22020713 | es |
dc.identifier.doi | 10.3390/ijms22020713 | es |
dc.journaltitle | International Journal of Molecular Sciences | es |
dc.publication.volumen | 22 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 713 | es |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (MICINN). España | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | es |