dc.creator | Bleriot, Inés | es |
dc.creator | Blasco, L. | es |
dc.creator | Delgado Valverde, María Mercedes | es |
dc.creator | Gual-de-Torrella, Ana | es |
dc.creator | Ambroa, A. | es |
dc.creator | Fernandez-Garcia, Laura | es |
dc.creator | Pascual Hernández, Álvaro | es |
dc.creator | Tomas, Maria | es |
dc.date.accessioned | 2020-12-18T19:01:09Z | |
dc.date.available | 2020-12-18T19:01:09Z | |
dc.date.issued | 2020-09-02 | |
dc.identifier.citation | Bleriot, I., Blasco, L., Delgado Valverde, M.M., Gual-de-Torrella, A., Ambroa, A., Fernandez-Garcia, L.,...,Tomas, M. (2020). Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK. Toxins, 12 (9), 1-17. | |
dc.identifier.issn | 2072-6651 | es |
dc.identifier.uri | https://hdl.handle.net/11441/103365 | |
dc.description.abstract | Although the failure of antibiotic treatment is normally attributed to resistance, tolerance
and persistence display a significant role in the lack of response to antibiotics. Due to the fact that
several nosocomial pathogens show a high level of tolerance and/or resistance to chlorhexidine,
in this study we analyzed the molecular mechanisms associated with chlorhexidine adaptation in two
clinical strains of Klebsiella pneumoniae by phenotypic and transcriptomic studies. These two strains
belong to ST258-KPC3 (high-risk clone carrying β-lactamase KPC3) and ST846-OXA48 (low-risk
clone carrying β-lactamase OXA48). Our results showed that the K. pneumoniae ST258-KPC3CA
and ST846-OXA48CA strains exhibited a different behavior under chlorhexidine (CHLX) pressure,
adapting to this biocide through resistance and tolerance mechanisms, respectively. Furthermore,
the appearance of cross-resistance to colistin was observed in the ST846-OXA48CA strain (tolerant to
CHLX), using the broth microdilution method. Interestingly, this ST846-OXA48CA isolate contained
a plasmid that encodes a novel type II toxin/antitoxin (TA) system, PemI/PemK. We characterized this
PemI/PemK TA system by cloning both genes into the IPTG-inducible pCA24N plasmid, and found
their role in persistence and biofilm formation. Accordingly, the ST846-OXA48CA strain showed
a persistence biphasic curve in the presence of a chlorhexidine-imipenem combination, and these
results were confirmed by the enzymatic assay (WST-1). | es |
dc.description.sponsorship | The State Plan for R+D+I 2013–2016 National Plan for Scientific Research, Technological Development and Innovation 2008–2011 PI16/01163 and PI19/00878 | es |
dc.description.sponsorship | ISCIII-Deputy General Directorate for Evaluation and Promotion of Research - European Regional Development Fund “A way of Making Europe” and Instituto de Salud Carlos III FEDER, Spanish Network for the Research in Infectious Diseases REIPI, RD16/0016/0001, RD16/CIII/0004/0002 and RD16/0016/0006 | es |
dc.description.sponsorship | The Study Group on Mechanisms of Action and Resistance to Antimicrobials, GEMARA | es |
dc.format | application/pdf | es |
dc.format.extent | 17 | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Toxins | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Tolerance | es |
dc.subject | Persistence | es |
dc.subject | Cross-resistance | es |
dc.subject | Toxin-antitoxin system | es |
dc.subject | PemI/PemK | es |
dc.subject | Klebsiella pneumoniae | es |
dc.title | Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.projectID | PI16/01163 | es |
dc.relation.projectID | PI19/00878 | es |
dc.relation.projectID | RD16/0016/0001 | es |
dc.relation.projectID | RD16/CIII/0004/0002 | es |
dc.relation.projectID | RD16/0016/0006 | es |
dc.relation.publisherversion | https://doi.org/10.3390/toxins12090566 | es |
dc.identifier.doi | 10.3390/toxins12090566 | es |
dc.journaltitle | Toxins | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 9 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 17 | es |