Artículo
Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain
Autor/es | León, Maela
Berbegal, Mónica Rodríguez Reina, José M. Elena, Georgina Abad Campos, Paloma Ramón Albalat, Antonio Olmo, Diego Vicent, Antonio Luque, Jordi Miarnau, Xavier Agustí Brisach, Carlos Trapero, Antonio Capote, Nieves Arroyo, Francisco T. Avilés Guerrero, Manuel Gramaje, David Andrés Sodupe, Marcos Armengol, Josep |
Departamento | Universidad de Sevilla. Departamento de Ciencias Agroforestales |
Fecha de publicación | 2020 |
Fecha de depósito | 2020-09-10 |
Resumen | Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond
orchards showing twig cankers and shoot blight symptoms in five different regions across Spain.
Multilocus DNA sequence analysis ... Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS, tub, tef-1α, cal and his), allowed the identification of four known Diaporthe species, namely: D. amygdali, D. eres, D. foeniculina and D. phaseolorum. Moreover, a novel phylogenetic species, D. mediterranea, was described. Diaporthe amygdali was the most prevalent species, due to the largest number of isolates (85.3%) obtained from all sampled regions. The second most frequent species was D. foeniculina (10.2%), followed by D. mediterranea (3.6%), D. eres and D. phaseolorum, each with only one isolate. Pathogenicity tests were performed using one-year-old almond twigs cv. Vayro and representative isolates of the different species. Except for D. foeniculina and D. phaseolorum, all Diaporthe species were able to cause lesions significantly different from those developed on the uninoculated controls. Diaporthe mediterranea caused the most severe symptoms. These results confirm D. amygdali as a key pathogen of almonds in Spain. Moreover, the new species, D. mediterranea, should also be considered as a potential important causal agent of twig cankers and shoot blight on this crop |
Cita | León, M., Berbegal, M., Rodríguez Reina, J.M., Elena, G., Abad Campos, P., Ramón Albalat, A.,...,Armengol, J. (2020). Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain. Agronomy, 2020. (10) (2020 (1062)), 1 p.-23 p.. |
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