Detection of DNA Double-Strand Breaks byγ-H2AX Immunodetection

Barroso, Sonia I.; Aguilera López, Andrés

Capítulo de Libro

dc.creator	Barroso, Sonia I.	es
dc.creator	Aguilera López, Andrés	es
dc.date.accessioned	2020-09-07T15:04:27Z
dc.date.available	2020-09-07T15:04:27Z
dc.date.issued	2020
dc.identifier.citation	Barroso, S.I., y Aguilera López, A. (2020). Detection of DNA Double-Strand Breaks byγ-H2AX Immunodetection. En Homologous Recombination Methods and Protocols (pp. 1-8). New York: Humana
dc.identifier.isbn	978-1-0716-0643-8	es
dc.identifier.issn	1064-3745	es
dc.identifier.issn	1940-6029	es
dc.identifier.uri	https://hdl.handle.net/11441/100776
dc.description.abstract	DNA double-strand breaks (DSBs) are the most deleterious type of DNA damage and a cause of geneticinstability as they can lead to mutations, genome rearrangements, or loss of genetic material when notproperly repaired. Eukaryotes from budding yeast to mammalian cells respond to the formation of DSBswith the immediate phosphorylation of a histone H2A isoform. The modified histone, phosphorylated inserine 139 in mammals (S129 in yeast), is namedγ-H2AX. Detection of DSBs is of high relevance inresearch on DNA repair, aging, tumorigenesis, and cancer drug development, given the tight association ofDSBs with different diseases and its potential to kill cells. DSB levels can be obtained by measuring levels ofγ-H2AX in extracts of cell populations or by counting foci in individual nuclei. In this chapter sometechniques to detectγ-H2AX are described.	es
dc.format	application/pdf	es
dc.format.extent	8 p.	es
dc.language.iso	eng	es
dc.publisher	Humana	es
dc.relation.ispartof	Homologous Recombination Methods and Protocols	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	DNA damage	es
dc.subject	Double-strand breaks	es
dc.subject	γ-H2AX	es
dc.subject	Immunofluorescence	es
dc.subject	Immunoblotting	es
dc.title	Detection of DNA Double-Strand Breaks byγ-H2AX Immunodetection	es
dc.type	info:eu-repo/semantics/bookPart	es
dcterms.identifier	https://ror.org/03yxnpp24
dc.type.version	info:eu-repo/semantics/publishedVersion	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	es
dc.contributor.affiliation	Universidad de Sevilla. Departamento de Genética	es
dc.relation.publisherversion	https://doi.org/10.1007/978-1-0716-0644-5	es
dc.publication.initialPage	1	es
dc.publication.endPage	8	es
dc.relation.publicationplace	New York	es

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