Artículo
Perturbing HIV-1 Ribosomal Frameshifting Frequency Reveals a cis Preference for Gag-Pol Incorporation into Assembling Virions
Autor/es | Benner, Bayleigh E.
Bruce, James W. Kentala, Jacob R. Murray, Magdalena Becker, Jordan T. García Miranda, Pablo Ahlquist, Paul Butcher, Samuel E. Sherer, Nathan M. |
Departamento | Universidad de Sevilla. Departamento de Fisiología |
Fecha de publicación | 2022 |
Fecha de depósito | 2023-11-13 |
Publicado en |
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Resumen | HIV-1 virion production is driven by Gag and Gag-Pol (GP) proteins, with Gag forming the bulk of the capsid and driving budding, while GP binds Gag to deliver the essential virion enzymes protease, reverse transcriptase, ... HIV-1 virion production is driven by Gag and Gag-Pol (GP) proteins, with Gag forming the bulk of the capsid and driving budding, while GP binds Gag to deliver the essential virion enzymes protease, reverse transcriptase, and integrase. Virion GP levels are traditionally thought to reflect the relative abundances of GP and Gag in cells (;1:20), dictated by the frequency of a 21 programmed ribosomal frameshifting (PRF) event occurring in gag-pol mRNAs. Here, we exploited a panel of PRF mutant viruses to show that mechanisms in addition to PRF regulate GP incorporation into virions. First, we show that GP is enriched ;3-fold in virions relative to cells, with viral infectivity being better maintained at subphysiological levels of GP than when GP levels are too high. Second, we report that GP is more efficiently incorporated into virions when Gag and GP are synthesized in cis (i.e., from the same gag-pol mRNA) than in trans, suggesting that Gag/GP translation and assembly are spatially coupled processes. Third, we show that, surprisingly, virions exhibit a strong upper limit to trans-delivered GP incorporation; an adaptation that appears to allow the virus to temper defects to GP/Gag cleavage that may negatively impact reverse transcription. Taking these results together, we propose a "weighted Goldilocks"scenario for HIV-1 GP incorporation, wherein combined mechanisms of GP enrichment and exclusion buffer virion infectivity over a broad range of local GP concentrations. These results provide new insights into the HIV-1 virion assembly pathway relevant to the anticipated efficacy of PRF-targeted antiviral strategies. |
Agencias financiadoras | National Institutes of Health. United States |
Identificador del proyecto | R01AI110221
P01CA022332 R35GM118131 T32GM008349 |
Cita | Benner, B.E., Bruce, J.W., Kentala, J.R., Murray, M., Becker, J.T., García Miranda, P.,...,Sherer, N.M. (2022). Perturbing HIV-1 Ribosomal Frameshifting Frequency Reveals a cis Preference for Gag-Pol Incorporation into Assembling Virions. Journal of Virology, 96 (1), e01349-21. https://doi.org/10.1128/JVI.01349-21. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Perturbing HIV-1.pdf | 2.990Mb | [PDF] | Ver/ | |