Artículo
Inhibition of Endothelial Inflammatory Response by HT-C6, a Hydroxytyrosol Alkyl Ether Derivative
Autor/es | Marrero, Ana Dácil
Castilla, Laura Bernal, Manuel Manrique, Inmaculada Posligua García, Joel D. Moya Utrera, Federico Porras Alcalá, Cristina Espartero Sánchez, José Luis ![]() ![]() ![]() ![]() ![]() ![]() Sarabia, Francisco Quesada, Ana R. Medina, Miguel Ángel Martínez Poveda, Beatriz |
Departamento | Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica |
Fecha de publicación | 2023 |
Fecha de depósito | 2023-09-11 |
Resumen | Hydroxytyrosol (HT) is a bioactive phenolic compound naturally present in olives and extra virgin olive oil (EVOO) which is described as an antioxidant, antitumoral and antiangiogenic molecule. Previous studies of ... Hydroxytyrosol (HT) is a bioactive phenolic compound naturally present in olives and extra virgin olive oil (EVOO) which is described as an antioxidant, antitumoral and antiangiogenic molecule. Previous studies of semi-synthetic HT-derivatives presented the hydroxytyrosyl alkyl ether HT-C6 as one of the most potent derivatives studied in the context of antioxidant, anti-platelet and antiangiogenic assays, but its direct effect on inflammation was not reported. In this work, we use RT-qPCR measure of gene expression, protein analysis by Western-blot and immunofluorescence techniques, adhesion and migration functional assays and single-cell monitoring of reactive oxygen species (ROS) in order to explore in vitro the ability of HT-C6 to interfere in the inflammatory response of endothelial cells (ECs). Our results showed that HT-C6 strongly reduces the TNF-α-induced expression of vascular cell adhesion molecule 1 (VCAM1), intercellular cell adhesion molecule 1 (ICAM1), E-selectin (SELE), C-C motif chemokine ligand 2 and 5 (CCL2 and CCL5) in HUVECs, impairing the chemotactic and adhesion potential of these cells towards THP-1 monocytes in vitro. In this work, we define a mechanism of action underlying the anti-inflammatory effect of HT-C6, which involves the abrogation of nuclear factor kappa B (NF-κB) pathway activation in ECs. These results, together with the ability of HT-C6 to reduce ROS formation in ECs, point to this compound as a promising HT-derivative to be tested in the treatment of atherosclerosis. |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Identificador del proyecto | PID2022-138181OB-I00
![]() PID2019-105010RB-I00 ![]() RTI2018-098296-BI00 ![]() IJC2018-037657-I ![]() UMA18-FEDERJA-220 ![]() PY20_00257 ![]() |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
Inhibition of Endothelial.pdf | 3.650Mb | ![]() | Ver/ | |