dc.creator | Marrero, Ana Dácil | es |
dc.creator | Castilla, Laura | es |
dc.creator | Bernal, Manuel | es |
dc.creator | Manrique, Inmaculada | es |
dc.creator | Posligua García, Joel D. | es |
dc.creator | Moya Utrera, Federico | es |
dc.creator | Porras Alcalá, Cristina | es |
dc.creator | Espartero Sánchez, José Luis | es |
dc.creator | Sarabia, Francisco | es |
dc.creator | Quesada, Ana R. | es |
dc.creator | Medina, Miguel Ángel | es |
dc.creator | Martínez Poveda, Beatriz | es |
dc.date.accessioned | 2023-09-11T15:07:56Z | |
dc.date.available | 2023-09-11T15:07:56Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 2076-3921 | es |
dc.identifier.uri | https://hdl.handle.net/11441/148867 | |
dc.description.abstract | Hydroxytyrosol (HT) is a bioactive phenolic compound naturally present in olives and extra virgin olive oil (EVOO) which is described as an antioxidant, antitumoral and antiangiogenic molecule. Previous studies of semi-synthetic HT-derivatives presented the hydroxytyrosyl alkyl ether HT-C6 as one of the most potent derivatives studied in the context of antioxidant, anti-platelet and antiangiogenic assays, but its direct effect on inflammation was not reported. In this work, we use RT-qPCR measure of gene expression, protein analysis by Western-blot and immunofluorescence techniques, adhesion and migration functional assays and single-cell monitoring of reactive oxygen species (ROS) in order to explore in vitro the ability of HT-C6 to interfere in the inflammatory response of endothelial cells (ECs). Our results showed that HT-C6 strongly reduces the TNF-α-induced expression of vascular cell adhesion molecule 1 (VCAM1), intercellular cell adhesion molecule 1 (ICAM1), E-selectin (SELE), C-C motif chemokine ligand 2 and 5 (CCL2 and CCL5) in HUVECs, impairing the chemotactic and adhesion potential of these cells towards THP-1 monocytes in vitro. In this work, we define a mechanism of action underlying the anti-inflammatory effect of HT-C6, which involves the abrogation of nuclear factor kappa B (NF-κB) pathway activation in ECs. These results, together with the ability of HT-C6 to reduce ROS formation in ECs, point to this compound as a promising HT-derivative to be tested in the treatment of atherosclerosis. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2022-138181OB-I00, PID2019-105010RB-I00, RTI2018-098296-BI00, IJC2018-037657-I | es |
dc.description.sponsorship | Junta de Andalucía UMA18-FEDERJA-220, PY20_00257 | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Antioxidant | es |
dc.subject | Atherosclerosis | es |
dc.subject | Endothelial cells | es |
dc.subject | HT-C6 | es |
dc.subject | Hydroxytyrosol | es |
dc.subject | Hydroxytyrosol derivatives | es |
dc.subject | Inflammatory response | es |
dc.subject | NF-κB | es |
dc.title | Inhibition of Endothelial Inflammatory Response by HT-C6, a Hydroxytyrosol Alkyl Ether Derivative | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica | es |
dc.relation.projectID | PID2022-138181OB-I00 | es |
dc.relation.projectID | PID2019-105010RB-I00 | es |
dc.relation.projectID | RTI2018-098296-BI00 | es |
dc.relation.projectID | IJC2018-037657-I | es |
dc.relation.projectID | UMA18-FEDERJA-220 | es |
dc.relation.projectID | PY20_00257 | es |
dc.relation.publisherversion | https://doi.org/10.3390/antiox12081513 | es |
dc.identifier.doi | 10.3390/antiox12081513 | es |
dc.journaltitle | Antioxidants | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 8 | es |
dc.publication.initialPage | 1513 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | Junta de Andalucía | es |