Datos de Investigación (Microbiología)
URI permanente para esta colecciónhttps://hdl.handle.net/11441/162323
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Dataset Differential Proteome of the BIOSISTEM Project [Dataset](2024) Reyes Pérez, Pedro José; Jiménez Guerrero, Irene; Medina Morillas, Carlos; Pérez Montaño, Francisco de Asís; Universidad de Sevilla. Departamento de Microbiología; Pérez Montaño, Francisco de Asís; Ministerio de Ciencia, Innovación y Universidades (MICINN). EspañaThe BIOSISTEM project aims to delve into the mechanisms that determine the specificity and efficiency of nodulation mediated by T6SS and its effectors in rhizobia, using as model organism Sinorhizobium fredii USDA257, a strain with one of the largest known host ranges. In addition, this project aims to determine the role of this protein secretion system in competition with other rhizospheric bacteria including some phytopathogens. The expected results of this project will improve the existing knowledge on the mechanisms responsible for rhizobium-legume compatibility, as well as the possible use of these microorganisms not only as biofertilizing agents but also as biocontrol agents. Rhizobia can thus become a reference for the design of biofertilizers with added value (biocontrol activity) for legumes, whose use constitutes one of the most important strategies for sustainable agriculture based on the reduction of the use of nitrogen fertilizers and chemical pesticides, in favor of the rational use of bacterial inoculants.
Dataset Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Spain: a culture-based detection analysis of raw and treated waters [Dataset](2024-09-06) Monge Olivares, Laura; Peñalva Moreno, Germán; Pulido, Marina R.; Garrudo Bárcena, Lara; Doval Aguirre, Miguel Ángel; Ballesta Mudarra, Sofía; Merchante, Nicolás; Rasero del Real, Pablo; Cuberos Gómez, Lucila; Carpes Hortal, Graciano; López Cerero, Lorena; Universidad de Sevilla. Departamento de Microbiología; López Cerero, Lorena; Junta de Andalucía. Consejería de Universidad, Investigación e Innovación; Fundación Francisco Soria Melguizo; Universidad de Sevilla. CTS 2010 Resistencia antimicrobianosBackground Antibiotics could modify multidrug-resistant microorganisms (MDRO) populations in urban wastewater. Water treatment plays a major role in their control. This study aimed to explain the characteristics of multidrug-resistant Gram-negative bacteria in the wastewater of a city in southern Spain, the reduction achieved by treatment and the influence of human antibiotic prescriptions. Methods We collected 24-hour composite water samples from four wastewater treatment plants (WWTPs) over one year (2021-2022) and cultured them quantitatively. All acquired ESBL/carbapenemase isolates were sequenced and genomes were compared with those of clinical isolates. Linear mixed model regression analyses were performed to assess the WWTP effect and the association with human antibiotic prescriptions. Findings Aeromonas predominated among the MDROs and KPC-2 was the main carbapenemase detected in the influents. The 44% and 30% of E. coli and K. pneumoniae influent isolates, respectively, belonged to high-risk clones; 10·6% of Enterobacteriaceae could be matched to clinical isolates and a strain from a current hospital outbreak was found in raw samples. WWTPs reduced ESBL and carbapenemase producers by 1·4-log and 1·8-log, respectively. New MDROs and persistence of certain strains were detected in effluents. Quinolone and cephalosporin prescriptions, flow-rate and population density were associated with higher OXA-48 producer counts. Interpretation Despite the reduction, additional technologies should be implemented in WWTPs receiving hospital discharges. Due to the predominance of environmental species, culture and metagenomic approaches should be combined to differentiate human and sewages origin for antibiotic resistance monitoring. Antibiotic stewardship could be a strategy to reduce the release of MDROs.