Datos de investigación (Bioquímica Médica y Biología Molecular e Inmunología)
URI permanente para esta colecciónhttps://hdl.handle.net/11441/161841
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Dataset Dataset of Olive seed protein hydrolysates possess antioxidant and anti-inflammatory activities in mice and human cells(2025-02-24) Cruz Chamorro, Iván; Bartolomei, Martina; Santos Sánchez, Guillermo; Ponce España, Eduardo; Álvarez López, Ana Isabel; Blázquez Nieto, Yaiza; García Rodríguez, Carlos; Bollati, Carlotta; d'Adduzio, Lorenza; Fanzaga, Melissa; Lammi, Carmen; Carrillo Vico, Antonio; Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología; Carrillo Vico, Antonio; Cruz Chamorro, Iván; Cruz Chamorro, Iván; Ministerio de Ciencia e Innovación (MICIN). España; Ministerio de Educación, Cultura y Deporte (MECD). España; Junta de Andalucía; Universidad de Sevilla. CTS160: Neuroinmunoendocrinología MolecularThe dataset includes the raw data carried out in the work titled "Olive seed protein hydrolysates possess antioxidant and anti-inflammatory activities in mice and human cells". The olive seed protein hydrolysates (OSPHs) demonstrated antioxidant and immunomodulatory effects on RAW 246.7 cells and human peripheral blood mononuclear cells, suggesting their potential for use in developing new functional foods or nutraceuticals. The aim of this work was to deepen the study of multifunctional behavior of OSPHs obtained from the Frantoio cultivar of Olea europaea L., prepared using two food-grade enzymes, namely Alcalase® (AH) and Papain (PH), studying their antioxidant capacity and possible immunomodulatory activity on a murine monocyte/macrophage cell line (RAW 246.7) and on the human peripheric blood mononuclear cells (PBMCs).
Dataset Dataset of Evaluation of the action of a lupine protein hydrolysate in Experimental Autoimmune Encephalomyelitis (EAE)(2025-01-22) Cruz Chamorro, Iván; Alvarez-Lopez, Ana Isabel; Santos Sánchez, Guillermo; Álvarez-Sánchez, Nuria; Pedroche, Justo; Millán-Linares, María del Carmen; Lardone, Patricia Judith; Carrillo Vico, Antonio; Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología; Carrillo Vico, Antonio; Cruz Chamorro, Iván; Cruz Chamorro, Iván; Ministerio de Economía y Competitividad (MINECO). España; Ministerio de Ciencia e Innovación (MICIN). España; Ministerio de Educación, Cultura y Deporte (MECD). España; Junta de Andalucía; Universidad de Sevilla; Universidad de Sevilla. CTS-160 : Neuroinmunoendocrinología MolecularThe dataset includes the raw data from the weight, clinical score, and number of sequences carried out in the work titled "A lupin (Lupinus angustifolius) protein hydrolysate decreases the severity of experimental autoimmune encephalomyelitis, a preclinical model of multiple sclerosis". LPH reduced the clinical score of the mice compared to the control group, as well as the maximum and cumulative scores, without changing the day of onset of the symptoms while the therapeutic intervention did not significantly improve the severity of the disease. Fifty-six female C57BL/6N mice (8-weeks-old) from the University of Seville Animal Facility were housed under standard conditions with ad libitum access to water and food. For the induction of EAE, 100 µg of MOG35–55 peptide (Cambridge Research Biochemicals, Cambridge, UK) emulsified in complete Freud´s adjuvant (CFA) containing 4 mg/mL of heat-inactivated Mycobacterium tuberculosis (Sigma-Aldrich, St. Louis, MO, USA) was inoculated subcutaneously. Furthermore, two doses of 400 ng pertussis toxin (Enzo Life Sciences, Farmingdale, NY, USA) were administered intraperitoneally on days 0 and 2 post-induction (p.i.) to induce the disease. An EAE clinical sign score was assigned daily to each mouse as follows: 0 = no clin-ical sign; 1 = no tail tone; 2 = impaired righting reflex; 3 = paralysis of one hind limb; 4 = paralysis of both hind limbs; 5 = paralysis of both hind limbs and one forelimb; 6 = if the score was greater than 5 (including moribund or dead mouse). LPH sequencing was performed using nano high-performance liquid chromatography coupled to an Orbitrap Elite mass spectrometer. The BIOPEP-UWM database was used to identify the motifs with proven biological activity.Dataset Evaluation of co-therapy with melatonin and methylprednisolone in Experimental Autoimmune Encephalomyelitis (EAE) [Dataset](2024-08-01) Álvarez López, Ana Isabel; Álvarez Sánchez, Nuria; Cruz Chamorro, Iván; Santos Sánchez, Guillermo; Ponce España, Eduardo; Bejarano, Ignacio; Lardone, Patricia Judith; Carrillo Vico, Antonio; Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología; Carrillo Vico, Antonio; Álvarez López, Ana Isabel; Álvarez López, Ana Isabel; Fundación Progreso y Salud; Junta de Andalucía; Universidad de Sevilla. CTS160: NeuroInmunoEndocrinología MolecularThe dataset includes the raw data from the clinical score and flow cytometry analyzes carried out in the work titled " Melatonin synergistically potentiates the effect of methylprednisolone on reducing neuroinflammation in the experimental autoimmune encephalomyelitis mouse model of multiple sclerosis". This study shows the protective synergistic effect of co-treatment with melatonin and methylprednisolone on reducing the severity of EAE by decreasing CD4+ lymphocytes, B cells, macrophages and dendritic cells in the CNS, as well as modulating the population of infiltrated T and B cells toward regulatory phenotypes to the detriment of pro-inflammatory effector functions. In addition, treatment with melatonin from the clinical onset of EAE improves the natural course of the EAE and the response to a subsequent treatment with methylprednisolone in a later relapse of the disease. Eight-week-old female C57BL/6N mice were immunized immunization with 100 μg of MOG35–55 (Cambridge Research Biochemicals, Cleveland) emulsified in CFA (Sigma) containing 50 μg of heat-killed Mycobacterium tuberculosis (H37Ra, ATCC 25177) by subcutaneous injection in both hind legs and two doses of intraperitoneal pertussis toxin (200 ng/day) (List Labs, California) on days 0 and 2 post-induction. Animals were randomly divided to receive melatonin at a concentration of 80 mg/kg and/or methylprednisolone at a concentration of 40 or 160 mg/kg in different treatment regimens. Mice were sacrificed at the peak of the disease (day 15 after induction) and after perfusion, the CNS was collected, homogenized and enzymatically dissociated with 1.87 mg/ml of collagenase IV (Worthington) and 0.25 mg/ml of DNase I (AppliChem) for 35 min at 37°C to obtain a suspension of single cells. Subsequently, a 37%:70% discontinous percoll gradient was carried out to isolate CNS-infiltrating mononuclear cells. To assess the profile of infiltrated immune cells in the CNS, cells were stained for the following antibodies against surface markers: CD45, CD4, CD8α, CD19, CD11b, CD11c, CD44, CD62L, B220, CD138, PD-1 (CD279), CTLA-4 (CD152), FAS (CD95), and CD25. To identify Treg and analyze intracellular production of TNF, IFN-γ and IL-10, after surface staining, cells were fixed and permeabilized using the The dataset includes the raw data from the clinical score and flow cytometry analyzes carried out in the work titled " Melatonin synergistically potentiates the effect of methylprednisolone on reducing neuroinflammation in the experimental autoimmune encephalomyelitis mouse model of multiple sclerosis". This study shows the protective synergistic effect of co-treatment with melatonin and methylprednisolone on reducing the severity of EAE by decreasing CD4+ lymphocytes, B cells, macrophages and dendritic cells in the CNS, as well as modulating the population of infiltrated T and B cells toward regulatory phenotypes to the detriment of pro-inflammatory effector functions. In addition, treatment with melatonin from the clinical onset of EAE improves the natural course of the EAE and the response to a subsequent treatment with methylprednisolone in a later relapse of the disease. Eight-week-old female C57BL/6N mice were immunized immunization with 100 μg of MOG35–55 (Cambridge Research Biochemicals, Cleveland) emulsified in CFA (Sigma) containing 50 μg of heat-killed Mycobacterium tuberculosis (H37Ra, ATCC 25177) by subcutaneous injection in both hind legs and two doses of intraperitoneal pertussis toxin (200 ng/day) (List Labs, California) on days 0 and 2 post-induction. Animals were randomly divided to receive melatonin at a concentration of 80 mg/kg and/or methylprednisolone at a concentration of 40 or 160 mg/kg in different treatment regimens. Mice were sacrificed at the peak of the disease (day 15 after induction) and after perfusion, the CNS was collected, homogenized and enzymatically dissociated with 1.87 mg/ml of collagenase IV (Worthington) and 0.25 mg/ml of DNase I (AppliChem) for 35 min at 37°C to obtain a suspension of single cells. Subsequently, a 37%:70% discontinous percoll gradient was carried out to isolate CNS-infiltrating mononuclear cells. To assess the profile of infiltrated immune cells in the CNS, cells were stained for the following antibodies against surface markers: CD45, CD4, CD8α, CD19, CD11b, CD11c, CD44, CD62L, B220, CD138, PD-1 (CD279), CTLA-4 (CD152), FAS (CD95), and CD25. To identify Treg and analyze intracellular production of TNF, IFN-γ and IL-10, after surface staining, cells were fixed and permeabilized using theDataset Anti-obesogenic effects of vegetable-derived protein hydrolysate [Dataset](2024-08-01) Ponce España, Eduardo; Cruz Chamorro, Iván; Santos Sánchez, Guillermo; Álvarez López, Ana Isabel; Fernández-Santos, José María; Pedroche, Justo; Millán Linares, María del Carmen; Bejarano Hernando, Ignacio; Lardone, Patricia Judith; Carrillo Vico, Antonio; ; Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología; Carrillo Vico, Antonio; Ponce España, Eduardo; Ponce España, Eduardo; Junta de Andalucía; Ministerio de Ciencia e Innovación (MICIN). España; Universidad de Sevilla; Ministerio de Educación, Cultura y Deporte (MECD). España; Universidad de Sevilla. CTS160: NeuroInmunoEndocrinología MolecularData was acquired for the study published in the article titled: Anti-obesogenic effect of lupin-derived protein hydrolysate through modulation of adiposopathy, insulin resistance and gut dysbiosis in a diet-induced obese mouse. This study examines the effects of a lupin protein hydrolysate (LPH) on obesity, adipose tissue dysfunction and gut dysbiosis in obese mice. LPH was characterized and protein concentration, ashes, moisture, fiber, fats, soluble sugars and phenol content were measured as described. Amino acid composition was determined using standard amino acid mix solution. The molecular weight profile was obtained by molecular exclusion chromatography. Eight-week-old male C57BL6/N mice were housed under standard conditions and mice were randomly assigned to three groups: mice fed a standard diet (SD), a high-fat diet (HFD) or an HFD and treated intragastrically with LPH (HFD+LPH) at a dose of 100mg/kg five days a week for 12 weeks. The SD and HFD groups were treated with vehicle under the same conditions as the HFD+LPH group. Food consumption and individual body weight were measured weekly. Animals were sacrificed with an intraperitoneal injection of sodium thiopental and blood was collected by cardiac puncture. Epidydimal adipose tissue (EpiWAT) was dissected, snap-frozen and stored at -80ºC until use or fixed in a 4% paraformaldehyde (PFA) solution. Stool was collected prior to euthanasia and stored at -80ºC. Serum biochemical profile was measured by chemoluminiscence in the Cobas Integra 400 (Roche Diagnostics, Indianapolis, IN, USA) at the Estación Biológica de Doñana (EBD-CSIC, Seville, Spain). Serum concentrations of leptin, adiponectin and insulin were quantified using enzyme-linked immunosorbent assays (ELISA) kits. Adipose tissue samples fixed in 4% PFA were dehydrated, embedded in paraffin blocks and sliced 4μm thick, stained with hematoxylin-eosin and cover mounted for posterior observation. Photomicrographs were obtained using Leica THUNDER microscope and Leica Application Suite X software. Adipocyte morphology and number of crown-like structures was blinded analyzed using ImageJ v1.53h public software. High throughput 16S rRNA gene amplicon analysis was performed using total DNA extracted from stool. 16S rRNA V3–V4 region was sequenced using MiSeq protocol and instruments. The raw sequences were processed using mothur.