Datos de Investigación (Cristalografía, Mineralogía y Química Agrícola)

URI permanente para esta colecciónhttps://hdl.handle.net/11441/169972

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    Dataset of From Polyurethane Waste to Biofertilizer: Metabolic Characterization of Rhodococcus pyridinivorans through Transcriptomic and Proteomic Analyses
    (2026-07-08) Orts Gómez, José María; Orts Gómez, Ángel; Naranjo Fernández, Emilia; Martín Presas, Luis; Tejada Moral, Manuel; Gómez Parrales, Isidoro Ángel; Castaño Navarro, Angélica; Parrado Rubio, Juan; Bioquímica y Biología Molecular; Cristalografía, Mineralogía y Química Agrícola; Orts Gómez, José María; Ministerio de Ciencia, Innovación y Universidades (MICIU). España; Agencia Estatal de Investigación. España; RNM365: Edafología Ambiental; AGR212: Tecnología y Aplicación de Enzimas
    A novel biofertilizer based on Rhodococcus pyridinivorans was developed through the bioconversion of depolymerized polyurethane (PU) foam, a highly recalcitrant polymeric waste. In this process, low-molecular-weight compounds released during PU depolymerization serve as carbon, nitrogen, and energy sources for bacterial growth, resulting in the production of biofertilizer biomass. This study aimed to characterize the metabolic adaptation of R. pyridinivorans to depolymerized PU and to identify the molecular mechanisms involved in the assimilation of PU-derived compounds and their conversion into bacterial biomass. To achieve this objective, an integrated metabolomic, transcriptomic, and proteomic approach was employed. Multi-omics analyses revealed the activation of oxidative, hydrolytic, transport-related, sulfur metabolism, and aromatic compound degradation pathways. These responses were accompanied by the expression of oxidases, hydrolases, and dehydrogenases involved in the transformation of polyols and oxidized intermediates into metabolites that feed central carbon metabolism. Together, these findings indicate extensive metabolic reprogramming that enables the efficient utilization of polyurethane-derived substrates. Our results demonstrate the remarkable metabolic plasticity of R. pyridinivorans and its ability to convert plastic-derived oxidation products into valuable biomass. These findings advance the mechanistic understanding of biological PU valorization and lay the groundwork for sustainable biotechnological recycling processes. Importantly, this approach enables the simultaneous conversion of plastic waste into a value-added agricultural product, highlighting a circular and sustainable route for polyurethane waste management.
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    Acceso abiertoConjunto de Datos
    Datos de Biorremediación aceite coche con bioestimulante
    (2026-04-13) Tejada Moral, Manuel; Gómez Parrales, Isidoro Ángel; Paneque Macías, Patricia Isabel; Gil Martínez, Marta; Orts Gómez, José María; Parrado Rubio, Juan; Cristalografía, Mineralogía y Química Agrícola; Bioquímica y Biología Molecular; Tejada Moral, Manuel; Ministerio de Ciencia e Innovación (MICIN). España; RNM365: Edafología Ambiental
    El trabajo consiste en la bioremediación de un suelo contaminado por aceite de motor de coche mediante el uso de un bioestimulante obtenido a partir de lodos de depuradora.
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    Dataset de Bioestimulante residuos cerveza tomate
    (2026-01-26) Tejada Moral, Manuel; Gómez Parrales, Isidoro Ángel; Orts Gómez, José María; Parrado Rubio, Juan; Cristalografía, Mineralogía y Química Agrícola; Bioquímica y Biología Molecular; Tejada Moral, Manuel; Ministerio de Ciencia e Innovación (MICIN). España; RNM365: Edafología Ambiental
    Biostimulants produced through enzymatic hydrolysis are agronomic tools of considerable interest, as they provide an alternative to the use of inorganic fertilisers. In this work, the effect of two biostimulants obtained from brewing industry by-products in a greenhouse tomato crop is studied. In addition to the subtilisine used to carry out the protein hydrolysis, one of the biostimulants incorporated an enzymatic cocktail degrade the β-glucans present in the by-products and transform them into free glucose. Both biostimulants were applied four times throughout the crop growth period via foliar and root applications at doses of 2.5 and 5 g L-1. During this period, plant height, fruit number, and the content of nutrients and photosynthetic pigments in leaves were measured. On the other hand, β-carotene and lycopene contents, equatorial diameter and mean fresh and dry weight were determined in the fruit. Finally, dehydrogenase activity was determined in the substrate. The results showed higher values when biostimulant without β-glucans at the higher dose was applied via root application, due to a greater root development and higher microbial activity in the substrate.
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    Dataset of oxifluorfen temperatura
    (2025-04-10) Paneque Macías, Patricia Isabel; Gómez Parrales, Isidoro Ángel; Parrado Rubio, Juan; Orts Gómez, José María; Tejada Moral, Manuel; Cristalografía, Mineralogía y Química Agrícola; Bioquímica y Biología Molecular; Tejada Moral, Manuel