Artículos (Ingeniería Química)

URI permanente para esta colecciónhttps://hdl.handle.net/11441/53665

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Lead-free hybrid perovskites: Structural and electronic analysis of MA0.5Rb0.5Bi0.5Ge0.25I3 and MA0.5Rb0.5Sb0.5Ge0.25I3
(Elsevier, 2025-04-05) García-Moreno, Fernando; Sánchez Coronilla, Antonio; Martín Fernández, Elisa Isabel; Universidad de Sevilla. Departamento de Química Física; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Ciencia, Innovación y Universidades (MICIU). España; Agencia Estatal de Investigación. España; European Union (UE)
Structural and electronic stability of MA0.5Rb0.5Bi0.5Ge0.25I3 and MA0.5Rb0.5Sb0.5Ge0.25I3 lead-free hybrid perovskites is addressed. The substitution of Pb in MAPbI3 perovskite is increasing the scientific attention due to its toxicity as well as stability of interest in the design of environmentally friendly solar cells. MA cation (CH3NH3 +) is replaced by 50 % Rb+ to improve stability and retain the organic characteristics. The substitution of lead in MA0.5Rb0.5PbI3 structure has been studied up to 100 % Ge. Electron localization function (ELF) analysis for structures with 75 and 100 % of Ge, show zones with no electron localization that indicates certain degree of structural instability. ELF and density of states (DOS) analysis of the structure MA0.5Rb0.5Pb0.5Ge0.5I3 with 50 % Ge corroborate stability characteristics of this perovskite. Thus, the MA0.5Rb0.5Pb0.5Ge0.5I3 structure has been selected for the substitution of lead by Bi and Sb. ELF and non-covalent index (NCI) analysis indicate Sb structures are slightly more stable than those with Bi. The presence of Bi and Sb drastically decreases the bandgap in the MA0.5Rb0.5Bi0.5Ge0.25I3 and MA0.5Rb0.5Sb0.5Ge0.25I3 structures, respectively, which makes both structures without lead of interest for use in photovoltaic devices. These findings provide a pathway for designing stable, lead-free perovskites with improved optoelectronic properties for next-generation solar cells.
Identification and effect of ozone and ultrasound pretreatments on Desmodesmus sp. and Tetradesmus obliquus proteins
(Elsevier, 2021-12) González Balderas, Regina M.; Velasquez-Orta, Sharon Belinda; Félix Ángel, Manuel; Bengoechea Ruiz, Carlos; Yañez-Noguez, Isaura; Orta Ledesma, María Teresa; Universidad de Sevilla. Departamento de Ingeniería Química; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Ministerio de Innovación, Ciencia y Empresa. España; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Either ozone or ultrasound pretreatment was applied to identify and evaluate their effect over the protein fraction of Desmodesmus sp. and Tetradesmus obliquus biomass cultivated in wastewater. Studies of SDS-PAGE, protein solubility, sulfhydryl and disulphide bond contents, and DSC were performed to evaluate the effect of each pretreatment on abundance, structure, and thermal properties of the protein concentrate obtained. Seven proteins were identified in Desmodesmus sp. biomass, while nine proteins were identified in Tetradesmus obliquus. The proteins identified may give a high add-value to microalgae biomass because they can find important applications in agricultural, food, and biomedical areas. On the other hand, the effect of pretreatment on these microalgal proteins mainly depends on protein structure. Desmodesmus sp. proteins showed a content of S-S groups an order of magnitude higher than Tetradesmus obliquus. This results in a higher glass transition temperature (Tg) for Desmodesmus sp. proteins (249 °C) than for Tetradesmus obliquus proteins (188 °C). On the contrary, the Tetradesmus obliquus native structure proteins tend towards cross-linking and protein-protein aggregation, as their primary structure has a high content of Methionine, Tyrosine, Tryptophan, Histidine, and Phenylalanine. Either ozone or ultrasound pretreatment, induces a Tg reduction in both Tetradesmus obliquus and Desmodesmus sp. proteins, however, this reduction is especially remarkable for Desmodesmus sp. proteins (drop in Tg of about 80 °C). The results showed that proteins in Desmodesmus sp. and Tetradesmus obliquus are highly resistant to pretreatment due to the nature of their biochemical structures which imply an excellent quality for protein valorisation, even after application of highly disruptive pretreatments.
Development of composites based on residual microalgae biomass cultivated in wastewater
(Elsevier, 2021-11-05) González Balderas, Regina M.; Félix Ángel, Manuel; Bengoechea Ruiz, Carlos; Orta Ledesma, María Teresa; Guerrero Conejo, Antonio Francisco; Velasquez-Orta, Sharon Belinda; Universidad de Sevilla. Departamento de Ingeniería Química; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Ministerio de Innovación, Ciencia y Empresa. España; Universidad de Sevilla; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Ultrasound pre-treatment and protein extraction of Desmodesmus sp. and Tetradesmus obliquus biomass induced residual microalgae/polycaprolactone (PCL) biocomposites with higher viscoelastic and mechanical properties as injection mould temperature increased. This was probably associated to the promotion of microalgae proteins-PCL interactions. The PCL content required, to strengthen the biocomposites, depended on the microalgae system (20 or 10 wt% for residual Desmodesmus sp. (RD) or Tetradesmus obliquus (RT), respectively). Protein degradation was observed in RT-based systems at mould temperatures higher than 100 °C. On the contrary, a greater mould temperature induced thermal crosslinking and certain cell disruption in RD-based systems. These environmentally-friendly biocomposites are an interesting alternative for replacing petroleum plastics.
Effect of Blending and Conjugation of Carboxymethyl Cellulose and Zein in Bioplastic Materials
(Springer, 2025-03) Alsadat-Seyedbokaei, Fahimeh; Félix Ángel, Manuel; Bengoechea Ruiz, Carlos; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Ciencia, Innovación y Universidades (MICIU). España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Carboxymethyl cellulose (CMC) can be extracted from agricultural waste and better employed in the formulation of bioplastics to promote sustainability. Zein, a hydrophobic prolamin protein that can be obtained from industrial wastes of the corn industry. It may be combined with hydrophilic CMC, resulting in composite materials where both functionalities are synergistically enhanced. In the zein/CMC systems studied in the present work, CMC was added directly in the mixing stage. Physical interactions take place as blends are formed, and the effect of the CMC concentration was studied from 5–30%. At the highest CMC concentration, those blend systems were compared to conjugated systems, where prior chemical conjugation of both biopolymers was carried out at 60°C for 48 h. The physical and chemical interactions between the biopolymers certainly affected the viscoelastic properties of the eventually obtained injection-moulded bioplastics. Thus, samples softened after the addition of CMC, independent of the procedure followed. Thus, the addition of CMC always resulted in a reduction in the viscoelastic moduli (i.e., E’ decreased from approximately 900 MPa in the absence of CMC to 265 MPa in the presence of 30% CMC, either blended or conjugated). Conversely, the samples presented much higher water uptake capacity (WUC) values when conjugation was carried out. Therefore, the WUC of zein bioplastics (approximately 200%) increased to 950% for 30% conjugated CMC, which is almost twice the value obtained when the same amount of CMC was added in the mixing stage. Biodegradable biocomposite materials obtained through conjugation could be of great interest for developing hydrophilic green materials.
Development of malt sprout-based bioplastics via injection-moulding
(Elsevier, 2021-04) Alonso González, María; Félix Ángel, Manuel; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Ciencia, Innovación y Universidades (MICIU). España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Millions of tons of malt sprouts, a low added-value by-product of the brewing industry, are produced after barley malting (germination of sprouting). Their valorisation can rise from their use for the development of bioplastics whose main source can be found in different wastes and by-products of the food industry, being an abundant, inexpensive and renewable resource available. The objective of this study is the development of bio-based, biodegradable bioplastics from malt sprout (MS), whose chemical composition accounts for great amounts of both proteins and starches. Different formulations (malt sprout/plasticizer ratios) were studied to determine the suitability of injection moulding processing, where the effect of injection pressure was evaluated. The mechanical properties of the bioplastics were evaluated by dynamic mechanical analysis (DMA) and tensile tests and some functional properties such as water uptake capacity (WUC) and soluble matter loss were also studied. Finally, the obtained properties were related to the bioplastics structure that was analysed via scanning electron microscopy. The tests carried out evidenced a clear dependence between mechanical properties and WUC of MS/plasticizer ratio and injection pressure, increasing the storage modulus (E’) from 0.94 MPa for 1:1 MS/plasticizer ratio processed at 500 bar to 1.42 and 4.30 MPa for 3:1 MS/plasticizer ratio processed at 500 and 900 bar, respectively. Moreover, the WUC increased from 232 ± 32 % for 1:1 MS/plasticizer ratio processed at 500 bar to 319 ± 33 and 412 ± 79 % for 3:1 MS/plasticizer ratio processed at 500 and 900 bar, respectively.
Development of bioplastic materials: From rapeseed oil industry by products to added-value biodegradable biocomposite materials
(Elsevier, 2018-12-01) Delgado, M.; Félix Ángel, Manuel; Bengoechea Ruiz, Carlos; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Economía y Competitividad (MINECO). España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Rapeseed crops are mainly harvested due to its high oil content. Production of oil from rapeseed generates a significant amount of by-products like presscake or meal. The high protein content (∼35%) of these rapeseed by-products makes them an interesting plant-derived alternative for the development of bioplastic materials. The generation of bioplastics from a rapeseed meal by injection moulding was studied herein at different mould temperatures (80, 100, 120 °C). Further processing of the meal (pelletizing, milling, sieving) on the bioplastics produced was also analysed using dynamic mechanical thermal analysis (DMTA), tensile tests and water uptake capacity. In all cases, strengthening of the samples occurred when moulding at high temperatures (120 °C), which might be related to thermally promoted protein cross-linking. This effect was reflected by an increase of 50% in the viscoelastic properties of the bioplastics when increasing the mould temperature from 80 to 120 °C. Biocomposites of rapeseed meal and polycaprolactone (PCL) at different PCL contents (0–20 wt. %) were also produced. The viscoelasticity of the biocomposites depended on PCL concentration. When PCL content was 20 wt.%, viscoelastic moduli (E’ and E’’) increased around 200%, which may be associated either to its role as a filler or to its integration into the protein matrix. These results indicate that rapeseed meal is a suitable alternative for the generation of bioplastic materials adding value to a by-product of the rapeseed oil industry.
Characterization of pea protein-based bioplastics processed by injection moulding
(Elsevier, 2016-01) Pérez-Puyana, Víctor Manuel; Félix Ángel, Manuel; Romero García, Alberto; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Economía y Competitividad (MINECO). España; Junta de Andalucía; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
This study assesses the behaviour of pea protein isolate (PPI) as a potential candidate for the development of biobased plastic materials processed by injection moulding. Around 30–40% of glycerol as plasticizer was required to obtain good processability of PPI/GL blends to produce bioplastics. A mixing rheometer that allows recording of torque and temperature during mixing and a small-scale-plunger-type injection moulding machine were used to obtain PPI/GL blends and PPI-based bioplastics, respectively. Rheological and differential scanning calorimetry measurements were made to guide the selection of suitable conditions for injection and moulding. For injection, we selected a temperature relatively close to the maximum of the loss tangent, but moderate enough to avoid crosslinking effects (50 °C), and for moulding, a high temperature (130 °C) to favour crosslinking in the mould. An increase in the PPI/GL ratio leads to an enhancement of elastic bending and tensile properties of bioplastic specimens, as well as an increase in their ability to absorb mechanical energy before rupturing. On the other hand, the PPI/GL specimens become less transparent. In addition, water uptake of these bioplastics has been found to be very high and fast.
Development of rice protein biobased plastic materials processed by injection molding
(Elsevier, 2016-01-01) Félix Ángel, Manuel; Lucio-Villegas Prieto, Álvaro; Romero García, Alberto; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Rice protein concentrate (RPC) has been evaluated as a potential candidate for the development of bio-based plastic materials processed by injection molding. Around 30% of glycerol (GL) as plasticizer and other additives (sodium bisulfite as a reducing agent as well as glyoxal and L-cysteine as cross-linking agents) were required to obtain good processability of RPC/GL blends to produce bioplastics. A mixing rheometer that allows recording of torque and temperature during mixing and a small-scale-plunger-type injection molding machine were used to obtain RPC/GL blends and RPC-based bioplastics, respectively. Rheological measurements were taken to guide the selection of suitable conditions for injection and molding. For injection, we selected a temperature relatively close to the glass transition temperature, but moderate enough to avoid crosslinking effects (87 °C), while for molding, we selected a higher temperature (130 °C) to favor crosslinking in the mold. However, other processing parameters (e.g., injection pressure) also need to be optimized. Final products (bioplastics) are plastic materials from renewable polymers (rice proteins) with both adequate properties for important industrial applications such as packaging, agriculture, etc. and high biodegradability when subjected to composting conditions. Adding each of the aforementioned additives leads to final specimens with different mechanical properties, as shown by dynamic mechanical temperature analysis and tensile strength measurements.
Effect of the injection moulding processing conditions on the development of pea protein-based bioplastics
(Wiley, 2016-02-05) Pérez-Puyana, Víctor Manuel; Félix Ángel, Manuel; Romero García, Alberto; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Economía y Competitividad (MINECO). España; Junta de Andalucía; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Bioplastic materials from renewable polymers, like proteins, constitute a highly interesting field for important industrial applications such as packaging, agriculture, etc., in which thermo-mechanical techniques are increasingly being used. Pea protein-based bioplastics can be made through a mixing process followed by an injection moulding. The objective of this study was to investigate the influence of different injection parameters (moulding time and injection pressure) on the properties exhibited by the final bioplastics obtained. A dynamic mechanical analysis and tensile strength measurements were performed, along with water absorption capacity and transparency tests. The results indicated that the major differences between bioplastics obtained at different moulding times are in transparency and in the Young's Moduli, exhibiting lower values as moulding time increases. On the other hand, modifying the injection pressure lead to more consistent bioplastics which differed mainly in the elastic component (E′ profiles) and in the strain at break. Furthermore, the water uptake was more than 100% in almost all the different bioplastics processed because of its hydrophilic character, so they could be considered as potential sources for absorbent material.
Development of crayfish protein-PCL biocomposite material processed by injection moulding
(Elsevier, 2015-09-01) Félix Ángel, Manuel; Romero García, Alberto; Martín Alfonso, José Enrique; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Junta de Andalucía; Ministerio de Economía y Competitividad (MINECO). España; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
A combination of crayfish flour (CF, with 60% protein) and Polycaprolactone (PCL) was successfully used to prepare biocomposites by a process that consists of two stages: mixing with glycerol (GL) as plasticizer and injection moulding of CF/GL/PCL blends. Mixing rheometry and Differential Scanning Calorimetry (DSC) measurements were found to be useful to select suitable injection moulding conditions. A remarkable enhancement in mechanical properties was found for PCL containing systems, even when crystalline structure remains unaltered. PCL yields a dominant contribution to the elastic response and confer a higher ability to absorb energy before rupture, but also the protein/plasticiser ratio must be considered.
Structured-illumination reflectance imaging for the evaluation of microorganism contamination in pork: effects of spectral and imaging features on its prediction performance
(Tsinghua University Press, 2025) Zhou, Binjing; Liu, Xiaohua; Ge, Yan; Tu, Kang; Peng, Jing; García-Martín, Juan Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Key Research Development Program of Shaanxi Province. China; Major Project of Science and Technology of Anhui Province. China; Joint Key Project of Science and Technology Innovation of Yangtze River; Delta in Anhui Province. China
Structured-illumination reflectance imaging (SIRI) provides a new means for food quality detection. This original work investigated the capability of (SIRI) technique coupled with multivariate chemometrics to evaluate the microbial contamination in pork inoculated with Pseudomonas fluorescens and Brochothrix thermosphacta during storage at different temperatures. The prediction performances based on different spectrum and the textural features of direct component and amplitude component images demodulated from the SIRI pattern, as well as their data fusion were comprehensively compared. Based on the full wavelength spectrum (420–700 nm) of amplitude component images, the orthogonal signal correction coupled with support vector machine regression provided the best predictions of the number of P. fl uorescens and B. thermosphacta in pork, with the determination coefficients of prediction (Rp 2 ) values of 0.870 and 0.906, respectively. Besides, the prediction models based on the amplitude component or direct component image textural features and the data fusion models using spectrum and textural features from direct component and amplitude component images cannot significantly improve their prediction accuracy. Consequently, SIRI can be further considered as a potential technique for the rapid evaluation of microbial contaminations in pork meat.
Simulation Tool for the Techno-Economic Assessment of the Integrated Production of Polyhydroxyalkanoates as Value-Added Byproducts of a Wastewater Treatment Plant
(MDPI, 2025) Pozo-Morales, Laura; Rosales Martínez, Antonio; Baquerizo, Enrique; del Valle Agulla, Germán; Universidad de Sevilla. Departamento de Ingeniería Química
The polyhydroxyalkanoate (PHA) production process that uses mixed microbial cultures combined with main stream wastewater treatment plants (WWTPs) is a competitive integrated resource recovery process in which non-oxygen electron acceptors can be used to enrich the PHA producer. Trials carried out in operating plants are very scarce, and there are no simulation tools available to analyse the feasibility of integrating the two processes. This research presents a novel analysis tool for a techno-economic assessment of value-added biopolymers. A general model for a conventional WWTP has been designed and eventually validated using the operating data collected in the database of a fully operational plant. In the model, a simulation of a PHA production line based on thickened primary sludge as a substrate has been integrated. The assembly has been treated as a closed-loop system with an accuracy level of 0.1% with a limit of 1000 iterations. Two strategies based on internal (ADF) or external (AN/AD) limitations of some nutrients have been contrasted for the selection of a biomass capable of feast–famine PHA synthesis. The ADF strategy was found to be the most favourable system, with a production of 0.226 kg of CODPHA·kg−1 COD. The calculated production cost was EUR 0.11·kg−1 CODPHA. The sludge production was reduced by 6%.
Interfacial properties of highly soluble crayfish protein derivatives
(Elsevier, 2016-06-20) Félix Ángel, Manuel; Romero García, Alberto; Vermant, Jan; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Junta de Andalucía; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
An analysis of the interfacial behaviour of two alternative protein derivatives with high solubility has been carried out at two different pH values (2 and 8). The protein derivatives (protein content higher than 50 wt.%) have a globular protein fraction mainly consisting of sarcoplasmic proteins (CFS); and a protein hydrolysate, (CFH), both obtained from crayfish surpluses. The physicochemical characterisation included protein solubility (higher than 80%) and surface charge as well as chemical analysis (elementary, sulfhydryl and disulphide groups). This was complemented with an interfacial characterization included measuring the surface pressure and surface dilatational measurements with a drop tensiometer, and interfacial shear rheology measurements performed using a double-wall-ring geometry. Whereas both types of protein derivative have significant interfacial activity (surface pressure between 20 and 40 mN/m), the difference in their behaviour is small In contrast, a strong influence of pH on the interfacial behaviour has been found for both soluble protein fractions. At pH 2, globular sarcoplasmic protein fraction yields lower surface pressures but highly viscoelastic interfaces, with 2D gel-like behaviour. At pH 8, on the other hand, CF proteins form quite rigid and more brittle interfaces, being resistant to dilatational deformations and showing high sensitivity to shear.
Influence of Transglutaminase (TGase) Enzyme on Mechanical and Bioactive Properties of Crayfish Protein Gels
(Springer, 2017-06-04) Félix Ángel, Manuel; Romero García, Alberto; Rustad, Turid; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Junta de Andalucía; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Functional and bioactive properties of crayfish meat convert their surpluses in an excellent alternative for the development of food products. Thus, protein dispersions were subjected to a thermal treatment, obtaining a protein-based gel. Rheological and antioxidant properties were studied at three different pH values (2.0, 6.5 and 8.0) when the TGase enzyme was used. The pH value exerted a strong influence on the gelation behaviour, as well as on the functional properties and the antioxidant activity of the final gels. The activity of the TGase enzyme is highly influenced by the pH of the protein dispersions. The highest antioxidant activity was obtained against ABTS and the lowest when FC reagent was used, whereas the activity against DPPH was also remarkable. TGase enzyme can be used during the thermal treatment to increase the mechanical properties, which were lost when hydrolysate systems were used.
Purification and Characterization of Transglutaminase Isolated from Sardine (Sardina pilchardus) Flesh Waste
(MDPI, 2025) Zaghbib, Imen; Abdullah, JAA; Hassouna, Mnasser; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Ciencia, Innovación y Universidades (MICINN). España; Agencia Estatal de Investigación. España; European Union (UE)
Transglutaminase (TGase) is an enzyme that catalyzes acyl transfer reactions by creating covalent cross-links between protein molecules and has been used to improve the physical and functional properties of protein-based foods. The objectives of this study were the extraction, purification, and biochemical characterization of TGase from sardine (Sardina pilchardus) flesh in order to provide a suitable TGase enzyme for food industry applications. The results showed a specific activity, yield, and purification fold of 357.14 U/mg protein, 36.74%, and 183.15, respectively. The enzyme exhibited maximal activity at 40 ◦C and pH 8.0, with a molecular weight of around 57 kDa. The effect of time on TGase thermal stability at 40 ◦C showed a gradual decrease in its catalytic activity during the incubation time until the enzyme was completely inactivated at 60 min. Additionally, the sardine TGase was found to be calcium-dependent. However, Mg2+ and Ba2+ ions were found to be effective in its activation to some extent and a total inhibition was shown by Zn2+ and Sr2+ ions. The TGase activity was affected markedly by NaCl and EDTA, and lost, respectively, about 80.7% and 36.49% from its activity by increasing the concentration (1.5 M NaCl and 20 mM EDTA). Based on the surface hydrophobicity and solubility results, the cross-linking of natural actomyosin mediated by TGase increased to a greater extent. The results revealed that sardine TGase possessed attractive qualities, making it a potential alternative to other TGase sources for food industry applications.
Development of thermally processed bioactive pea protein gels: Evaluation of mechanical and antioxidant properties
(Elsevier, 2017-01) Félix Ángel, Manuel; Pérez-Puyana, Víctor Manuel; Romero García, Alberto; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte; Junta de Andalucía; Ministerio de Economía y Competitividad (MINECO). España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Thermal processing of plant proteins (e.g. from pea) is highly interesting due to its potential in the generation of new textures in food products. This processing route is particularly attractive when it is combined with the revalorization entailed by the bioactive properties of the processed gels. In fact, the antioxidant activity of food products, which has been found in numerous proteins and peptides from protein hydrolysates, is widely demanded by the society since it prevents the development of serious diseases. This work is focused on the evaluation of mechanical and antioxidant properties from a Pea Protein Concentrate (PPC) and two hydrolysates obtained from PPC (PPH₂₅ and PPH₁₂₀) at three different pH values (2.0, 6.5 and 8.0). The gelation process was monitored and, subsequently, mechanical spectra were obtained. In addition, protein interactions (ionic interactions, hydrophobic interactions, hydrogen bonds and disulphide bonds) were also determined in order to elucidate their contribution to the formation of the gel network. Finally, the characterisation of the antioxidant activity of the gels was carried out using three different reagents: DPPH, ABTS and Folin–Ciocalteu. Results put forward that each variable studied, the degree of hydrolysis and pH, are key factors over the thermal processing of gels, showing a remarkable influence on both mechanical properties and antioxidant activity.
Influence of pH and Xanthan Gum on long-term stability of crayfish-based emulsions
(Elsevier, 2017-11) Félix Ángel, Manuel; Romero García, Alberto; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Junta de Andalucía; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
The present work focus on the evaluation of crayfish protein concentrates, surplus from crayfish processing industry, in the formation and stabilization of high-oleic O/W emulsions as a function of pH values (3.0, 5.0 and 8.0) and Xanthan Gum (XG) concentrations (0.06, 0.12, 0.25 and 0.50 wt.%). The emulsion microstructure and stability were determined through droplet size distributions (DSD) and rheological measurements, which were determined by means of Small Amplitude Oscillatory Shear (SAOS) measurements, over the ageing time. In addition, backscattering measurements were carried out to describe the destabilization mechanism. DSD profiles confirm that emulsions at pH 3.0 are not flocculated and they are stable over the ageing time studied. However, at pH 5.0 and 8.0 flocculation and further coalescence phenomena takes place. Rheological characterisation reveals a strong dependence of linear viscoelastic properties on XG concentration. In addition, viscoelastic properties also suggest that electrostatic interactions leads to the formation of an enhanced protein/polysaccharide network at pH 3.0.
Evaluation of the injection moulding conditions in soy/nanoclay based composites
(Elsevier, 2017-10) Bourny, Valentin; Félix Ángel, Manuel; Pérez-Puyana, Víctor Manuel; Romero García, Alberto; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Economía y Competitividad (MINECO). España; Universidad de Sevilla; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Biocomposites, and generally bio-based plastics, are seen as a promising alternative to conventional plastics because they are based on renewable natural components (eco-friendly) with a lower cost. Biocomposites using soy protein and glycerol were obtained with an injection moulding technique as the thermoprocessing method. Nanoclay (Montmorillonite, MMT-Na⁺) was included to improve physicochemical properties of novel SPI/MMT nanocomposites prepared by injection moulding, primary attending to water uptake capacity (WUC), but also considering rheological and mechanical properties. The influence of processing conditions has been studied in order to assess the modifications taking place in the microstructure and properties of these nanocomposites. Addition of MMT-Na⁺ improves mechanical properties and WUC of SPI-based materials, depending on processing conditions. Injection pressure only exerts a slight enhancement of mechanical properties and WUC. However, an increase in mixing time leads to a relevant increase in WUC without impairing the mechanical properties of nanocomposites.
Development of protein-based bioplastics modified with different additives
(Wiley, 2017-11-05) Félix Ángel, Manuel; Pérez-Puyana, Víctor Manuel; Romero García, Alberto; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte; Ministerio de Economía y Competitividad (MINECO). España; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Proteins have been postulated as a feasible source for manufacturing biodegradable polymeric materials. The aim of this study is the development of bioplastic materials from two different protein sources: albumen protein isolated (API), which consists of globular proteins, and crayfish flour (CF), mostly composed of myofibrillar proteins. In order to explore the effect of some chemical reagents on the mechanical properties of the blends and bioplastic materials, two different additives have been used: sodium sulfite (SS) and urea (U). The first one is a reducing agent, and the second one is considered a denaturing agent. The addition of chemical agents induces changes not only in mechanical properties but also in the most suitable processing conditions, which strongly depends on the protein used. Thus, the denaturation of globular proteins seems to lead to a more consistent blend before the injection-molding process. However, when myofibrillar proteins are used, the processability of the dough-like material increases after using either SS or U additives. This work illustrates the feasibility of producing animal-based biodegradable bioplastic materials with different properties and, consequently, different applications, which contribute to adding a high value to two different byproducts from the food industry.
Physicochemical, microstructure and bioactive characterization of gels made from crayfish protein
(Elsevier, 2017-02) Félix Ángel, Manuel; Romero García, Alberto; Rustad, Turid; Guerrero Conejo, Antonio Francisco; Universidad de Sevilla. Departamento de Ingeniería Química; Junta de Andalucía; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
Crayfish proteins are valuable active ingredients for food products, mainly due to its protein quality and antioxidant activity. A highly soluble crayfish protein concentrate (CF2L) obtained from crayfish surpluses was used to evaluate gelling properties at three different pH values (2.0, 6.5 and 8.0). Thermal gelation processes were monitored by Small Amplitude Oscillatory Shear (SAOS) measurements. Subsequently, gels were characterized by viscoelastic properties, water-holding capacity (WHC) and a selective solubility. All the systems exhibited a gel-like behavior, showing a strong dependence on pH. Antioxidant measurements were performed by using three different reagents (DPPH, ABTS and Folin-Ciocalteu) and revealed an interesting potential for human food. The pH effect was evaluated, showing a remarkable dependence of ABTS on its value. The results show that gels made from crayfish surpluses have potential for use in human nutrition, not only based on the physical properties, but also on its protein quality and antioxidant ability.

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