Capítulos (Microbiología)

URI permanente para esta colecciónhttps://hdl.handle.net/11441/86159

Examinar

Mostrando 1 - 5 de 5

A Novel System to Selective Tagging of Sinorhizobium fredii Symbiotic Plasmids
(Humana, 2024) Cutiño, Ana María; Sánchez Aguilar, María del Carmen; Ruiz Sainz, José Enrique; Espuny Gómez, María del Rosario; Ollero Márquez, Francisco Javier; Medina Morillas, Carlos; Microbiología; Medina Morillas, Carlos; López Baena, Francisco Javier; Ministerio de Ciencia e Innovación (MICIN). España; European Union (UE)
Conventional systems used to tag and transfer symbiotic plasmids (pSyms) of rhizobial strains are based in mutagenesis with transposons. In those processes numerous clones must be analysed to find one of them with the transposon inserted in the pSym. Following this strategy, the insertion might interrupt a gene that can affect the symbiotic phenotype of the bacteria tagged. Here, we have developed a new system based in homologous recombination, that generates Sinorhizobium fredii strains with pSyms tagged by the insertion of a suicide vector which harbour a truncated copy of S. fredii HH103 nodZ gene, a mob site and a kanamycine resistant gene. When it is introduced by conjugation in a S. fredii strain, the vector integrates in pSym by only one recombination event. This pSym tagged can be transferred in matting experiments to other strains in the presence of a helper plasmid. Following this method, we have tagged several strains and transfer their pSyms to a recipient strain demonstrating the potential of this new system.
Methods for studying swimming and surface motilities in rhizobia
(Humana, 2024) Fuentes Romero, Francisco; Alias Villegas, Cynthia; Navarro Gómez, Pilar; Acosta Jurado, Sebastián; Bernabéu Roda, Lydia M.; Cuéllar, Virginia; Soto, María J.; Vinardell González, José María; Microbiología; Ministerio de Ciencia e Innovación (MICIN). España; Agencia Estatal de Investigación. España; European Union (UE); Junta de Andalucía
Rhizobia are soil proteobacteria able to establish a nitrogen-fixing interaction with legumes. In this interaction, rhizobia must colonize legume roots, infect them, and become hosted inside new organs formed by the plants and called nodules. Rhizobial motility, not being essential for symbiosis, might affect the degree of success of the interaction with legumes. Because of this, the study of rhizobial motility (either swimming or surface motility) might be of interest for research teams working on rhizobial symbiotic performance. In this chapter, we describe the protocols we use in our laboratories for studying the different types of motilities exhibited by Sinorhizobium fredii and Sinorhizobium meliloti, as well as for analysing the presence of flagella in these bacteria. All these protocols might be used (or adapted) for studying bacterial motility in rhizobia.
Virulence-Related Assays for Investigation of the Acidovorax citrulli-Cucurbitaceae Pathosystem
(Humana, New York, NY, 2024) Pérez Montaño, Francisco de Asís; Jiménez Guerrero, Irene; Tamir-Ariel, Dafna; Burdman, Saul; Microbiología; United States-Israel Binational Agriculture Research and Development (BARD); Ministerio de Ciencia e Innovación (MICIN). España
Acidovorax citrulli is one of the most important pathogens of cucurbit crops, mainly melon and watermelon. Although A. citrulli is able to infect all aerial parts of the plant, fruits are highly sensitive to the bacterium. Therefore, the disease is known as bacterial fruit blotch (BFB). The unavailability of effective tools for managing BFB, including the lack of resistant varieties, exacerbates the threat this disease poses to the cucurbit industry. However, despite the economic importance of BFB, still little is known about basic aspects of A. citrulli–plant interactions. Here, we present diverse techniques that have recently been developed for investigation of basic aspects of BFB, including identification of virulence determinants of the pathogen.
A Robust Method to Perform In Vitro and In Planta Interbacterial Competition Assays: Killing Plant Pathogens by a Potent Biocontrol Agent
(Springer, 2024) Civantos Jiménez, Cristina; Ruiz Camas, Adrián; Bernal Guzmán, Patricia; Microbiología; Ministerio de Ciencia e Innovación (MICIN). España; European Union (UE); European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); BIO169: Biotecnología de la Interacción de Microorganismos con Leguminosas y Otras Plantas de Interés Agrícola
Interbacterial competition assays have become an essential tool for understanding the interactions between bacteria and their ability to outcompete one another in natural environments. This is especially relevant when studying the type VI secretion system (T6SS), a contact-dependent bacterial weapon that can be used to kill or inhibit the growth of other competing bacteria. Some beneficial environmental microorganisms such as Pseudomonas putida rely on the T6SS as their primary biocontrol mechanism to eliminate resilient plant pathogens. Competition assays are an essential methodology in this field that allows us to understand the efficacy of this bacterial nanoweapon. This chapter outlines the methodology for conducting in vitro and in planta competition assays between P. putida, a well-known biocontrol agent, and phytopathogenic bacterial species of economic and scientific interest.
Programa de Innovación docente en la asignatura de Microbiología I
(Editorial Universidad de Sevilla, 2020) Bernal Guzmán, Patricia; Microbiología

Examinar

Envíos recientes