Capítulos (Microbiología)
URI permanente para esta colecciónhttps://hdl.handle.net/11441/86159
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Capítulo de Libro Virulence-Related Assays for Investigation of the Acidovorax citrulli-Cucurbitaceae Pathosystem(Humana, New York, NY, 2024) Pérez Montaño, Francisco de Asís; Jiménez Guerrero, Irene; Tamir-Ariel, Dafna; Burdman, Saul; Universidad de Sevilla. Departamento de Microbiología; United States-Israel Binational Agriculture Research and Development (BARD); Ministerio de Ciencia e Innovación (MICIN). EspañaAcidovorax citrulli is one of the most important pathogens of cucurbit crops, mainly melon and watermelon. Although A. citrulli is able to infect all aerial parts of the plant, fruits are highly sensitive to the bacterium. Therefore, the disease is known as bacterial fruit blotch (BFB). The unavailability of effective tools for managing BFB, including the lack of resistant varieties, exacerbates the threat this disease poses to the cucurbit industry. However, despite the economic importance of BFB, still little is known about basic aspects of A. citrulli–plant interactions. Here, we present diverse techniques that have recently been developed for investigation of basic aspects of BFB, including identification of virulence determinants of the pathogen.
Capítulo de Libro A Robust Method to Perform In Vitro and In Planta Interbacterial Competition Assays: Killing Plant Pathogens by a Potent Biocontrol Agent(Springer, 2024) Civantos Jiménez, Cristina; Ruiz Camas, Adrián; Bernal Guzmán, Patricia; Universidad de Sevilla. Departamento de Microbiología; Ministerio de Ciencia e Innovación (MICIN). España; European Union (UE); European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Universidad de Sevilla. BIO169: Biotecnología de la Interacción de Microorganismos con Leguminosas y Otras Plantas de Interés AgrícolaInterbacterial competition assays have become an essential tool for understanding the interactions between bacteria and their ability to outcompete one another in natural environments. This is especially relevant when studying the type VI secretion system (T6SS), a contact-dependent bacterial weapon that can be used to kill or inhibit the growth of other competing bacteria. Some beneficial environmental microorganisms such as Pseudomonas putida rely on the T6SS as their primary biocontrol mechanism to eliminate resilient plant pathogens. Competition assays are an essential methodology in this field that allows us to understand the efficacy of this bacterial nanoweapon. This chapter outlines the methodology for conducting in vitro and in planta competition assays between P. putida, a well-known biocontrol agent, and phytopathogenic bacterial species of economic and scientific interest.Capítulo de Libro Programa de Innovación docente en la asignatura de Microbiología I(Editorial Universidad de Sevilla, 2020) Bernal Guzmán, Patricia; Universidad de Sevilla. Departamento de Microbiología