Datos de Investigación (Microbiología y Parasitología)

URI permanente para esta colecciónhttps://hdl.handle.net/11441/170020

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    EmbargoDataset
    Experimental data on sequence variability in Salmonella
    (2025-06-22) Fernández Fernández, Rocío; Gutiérrez Pozo, Gabriel; Piubeli, Francine; Garmendia, Junkal; Beuzón, Carmen R.; Sánchez Romero, María Antonia; Microbiología y Parasitología; Genética; Sánchez Romero, María Antonia; Ministerio de Ciencia, Innovación y Universidades (MICIU). España
    This dataset was generated as a part of a study investigating the mechanisms of bacterial adaptation, with particular emphasis on sequence variability within promoter regions of surface-related genes in Salmonella. The work focused on the opvAB operon, whose transcription is epigenetically regulated through Dam-mediated methylation of GATCs sites and OxyR binding, and plays a key role in Salmonella adaptation under selective pressures such as bacteriophages presence. The dataset, which combines bioinformatic analyses with laboratory experiments, is organized into several files: • BLASTn results: .xlsx file containing alignment information obtained from BLASTn searches using the regulatory sequences (RS) and coding sequences (CDS) of surface-related and housekeeping genes from Salmonella. • G+C content analysis: .dna file containing the sequence of the opvAB operon along with 1.5 kb of upstream and downstream flanking regions. This file enables the identification of the opvAB-containing sequence potentially acquired through horizontal gene transfer by assessing differences in G+C content when compared to the characteristic composition of the Salmonella genome. • Prevalence of opvAB in the genus Salmonella: .xlsx file quantifying the presence of the opvAB operon in Salmonella genomes available in the NCBI database, organized by species (green) and subspecies (purple). • Analysis of regulatory elements diversity across Salmonella enterica subspecies: .xlsx files detailing the conservation of the opvAB promoter's regulatory elements (-35 and -10 boxes, four GATCs and four OxyR Binding Sites [OBSs]) across the enterica, arizonae, diarizonae and salamae subspecies. • Nucleotide composition of OBSs across Salmonella enterica subspecies: .xlsx file showing the percentage of adenine (A), guanine (G), cytosine (C), and thymine (T) nucleotides for each OxyR binding site (OBSs) across the enterica, arizonae, diarizonae and salamae subspecies. • Analysis of mutational distribution within the opvAB regulatory region: .xlsx file reporting the location of mutations in the opvAB promoter across Salmonella enterica subsp. enterica genomes containing up to eight mutations, as well as in environmental strains isolated from poultry farms. Sequence variations are annotated using a numerical code: 0=conserved position, 1=substitution, 2=insertion, and 3=deletion. • Flow Cytometry Data: .lmd files and an associated .xlsx file showing the percentage of cells expressing the opvAB operon (OpvABON).
  • Miniatura
    EmbargoDataset
    Dataset Salicornia ramosissima Cu (0-1000 ppm) x salt (0-10-60 g/L) x bacteria
    (2025-09-17) Mesa Marín, Jennifer; Redondo Gómez, Susana; Rodríguez Llorente, Ignacio David; Pajuelo Domínguez, Eloísa; Mateos Naranjo, Enrique; Microbiología y Parasitología; Biología Vegetal y Ecología; Mesa Marín, Jennifer; Mesa Marín, Jennifer; Mesa Marín, Jennifer; Mateos Naranjo, Enrique; Junta de Andalucía; European Union (UE); Ministerio de Ciencia, Innovación y Universidades (MICIU). España; Agencia Estatal de Investigación. España; BIO181: Fitomicrobiomas Como Herramientas Biotecnológicas; RNM035: Ecología Funcional Aplicada
    Data of growth (weight, height and water content), photosynthesis parameters (fluorescence (Fv/Fm), stomatal conductance (gs), intercellular CO2 concentration (Ci), net photosynthetic rate (AN) and instantaneous water use efficiency (iWUE)) and nutrient content in the halophyte Salicornia ramosissima, after an experimental design with three different variables in factorial combination: two copper concentrations (0 and 1000 ppm), three NaCl concentrations (0 g L-1, 10 g L-1, 60 g L-1) and five inoculants (non-inoculated and four combinations of PGPR strains) (n = 300, 30 treatments with 10 plants per treatment).