Artículos (Instituto de Biomedicina de Sevilla (IBIS))

URI permanente para esta colecciónhttps://hdl.handle.net/11441/11095

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Analysis of dry needling combined with an exercise program in the treatment of knee osteoarthritis: A randomized clinical trial
(MDPI, 2024-11-26) Agost-González, Aida; Escobio Prieto, Isabel; Barrios-Quinta, Cristo Jesús; Cardero-Durán, María de los Ángeles; Espejo-Antúnez, Luis; Albornoz Cabello, Manuel; Universidad de Sevilla. Departamento de Fisioterapia; Universidad de Sevilla. CTS-1137: NEUROPhysiUS - Neurological Physiotherapy, Innovative Neurorehabilitation and Neurodevelopmental Disorders; Universidad de Sevilla. CTS-947: Salud y actividad física para calidad de vida
Background: Therapeutic exercise is recommended for people with knee osteoarthritis (OA), although it could be complemented with other treatments such as dry needling (DN). The purpose of this study was to evaluate and compare the resulting data on pain, functionality, strength and range of motion in subjects with knee osteoarthritis after being treated with a specific therapeutic physical exercise program alone or in combination with the DN technique in the popliteus muscle. Methods: A total of 33 participants were randomly assigned to two groups: the dry needling plus therapeutic physical exercise group (n = 15) and the therapeutic physical exercise alone group (n = 18). Both groups received the same exercise protocol, and the dry-needling group conducted three sessions of this technique over 3 weeks. Results: Variables such as pain, functionality, neuropathic pain, stiffness, strength, range of motion, pain catastrophizing and kinesiophobia were evaluated before and after the intervention, as well as at a follow-up 3 months after the intervention. Significant differences were observed between the two groups in pain intensity, stiffness, functionality, pain catastrophizing and kinesiophobia (p < 0.001). Conclusions: The combination of dry needling targeting the popliteus muscle and therapeutic physical exercise showed better results in terms of pain, functionality and strength compared to therapeutic physical exercise alone, especially after the intervention.
Mediterranean diet combined with regular aerobic exercise and hempprotein supplementation modulates plasma circulating amino acids and improves the health status of overweight individuals
(Mdpi, 2024-05-23) Miguel Albarreal, Antonio de; Rivero-Pino, Fernando; Márquez Parada, Elvira; Grao Cruces, Elena; González de la Rosa, Teresa; Montserrat de la Paz, Sergio; Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología; Junta de Andalucía; Gobierno de España; Universidad de Sevilla. CTS 1074: InmunoNutrición e InmunoMetabolismo.
Plant protein is considered a sustainable health-promoting strategy to prevent metabolic syndrome. Lifestyle changes (including dietary patterns and exercise) have been demonstrated to exert an effect on human health by modulating the biochemical status in humans. The objective of this study was to assess whether supplementation with hemp protein within a Mediterranean diet context together with exercise could help to ameliorate the metabolic statuses of patients prone to developing metabolic syndrome. For this study, 23 patients followed with Mediterranean diet and engaged in aerobic exercise according to the WHO’s recommendations, while also being supplemented with hemp protein, for 12 weeks. A comparison of anthropometric, biochemical, and mineral data as well as amino acid values was made between the start and the end of the study, with the subjects acting as their own control group. Statistical analyses included a paired t-test, Wilcoxon paired test, Pearson correlation coefficient, and Sparse Partial Least Squares Discriminant Analysis to evaluate significant differences and correlations among parameters. There were statistically significant changes in total cholesterol, HDL-C (+52.3%), LDL-C (−54.0%), and TAG levels (−49.8%), but not in glucose plasma levels. Following the intervention, plasma concentrations of some amino acids, including α-aminoadipic acid, phosphoethanolamine, and 1-metylhistidine, increased, whereas those of asparagine and alanine declined. Different correlations between amino acids and the other parameters evaluated were reported and discussed. A Mediterranean diet combined with regular aerobic exercise, together with protein supplementation, can highly improve the metabolic parameters and anthropometric parameters of subjects with obesity and impaired glucose levels, ameliorating their health status and likely delaying the development of metabolic syndrome
Study of the impact on zygomatic bone using numerical simulation
(MDPI, 2024-11-14) Ruiz-de-León, Gonzalo; Baus Domínguez, María; González Martín, María Isabel; Gutiérrez Corrales, Aída; Torres-Carranza, Eusebio; Martínez-González, Álvaro-José; Torres-Lagares, Daniel; López Millán, Jose Manuel; Ambrosiani Fernández, Jesús; Universidad de Sevilla. Departamento de Estomatología; Universidad de Sevilla. Departamento de Cirugía; Universidad de Sevilla. Departamento de Anatomía y Embriología Humana; Universidad de Sevilla. CTS-1050: Cuidados Complejos, Cronicidad y Resultados en Salud; Universidad de Sevilla. CTS-523: Innovación y desarrollo en técnicas y fundamentos de cirugía bucal y craneofacial; Universidad de Sevilla. CTS-1037: Abordaje multidisciplinar de la patología rinosinusal, otológica y de base de cráneo
The zygomatic bone, a fundamental structure in facial anatomy, is exposed to fractures in impact situations, such as traffic accidents or contact sports. The installation of zygomatic implants can also alter the distribution of forces in this region, increasing the risk of fractures. To evaluate this situation, the first step is to develop a complex anatomical model from the stomatognathic point of view so that simulations in this sense can be validated. This study uses numerical simulation using a finite-element method (FEM) to analyze the behavior of the zygomatic bone under impacts of different velocities, offering a more realistic approach than previous studies by including the mandible, cervical spine, and masticatory muscles. Methods: An FEM model was developed based on 3D scans of actual bones, and simulations were performed using Abaqus Explicit 2023 software (Dassault Systemes, Vélizy-Villacoublay, France). The impact was evaluated using a steel cylinder (200 mm length, 40 mm diameter, 2 kg weight) impacted at speeds of 5, 10, 15, and 20 km/h. Zygomatic, maxillary, and mandibular bone properties were based on dynamic stiffness parameters, and bone damage was analyzed using ductile fracture and fracture energy criteria. Results: The results show that at impact velocities of 15 and 20 km/h, the zygomatic bone suffered crush fractures, with impact forces up to 400 kg. At 10 km/h, a combination of crushing and bending was observed, while at 5 km/h, only local damage without complete fracture was detected. The maximum stresses were concentrated at the zygoma–jaw junction, with values above 100 MPa at some critical points. Conclusion: The FEM model developed offers a detailed representation of the mechanical behavior, integrating the main structures of the stomatognathic apparatus of the zygomatic bone under impact, providing valuable information to, for example, advance injury prevention and zygomatic implant design. Higher impact velocities result in severe fractures, underscoring the need for protective measures in clinical and sports settings.
Intervention strategies to reduce maternal mortality in the context of the sustainable development goals: a scoping review
(MDPI, 2024-10-28) Olea-Ramirez, Lucia Macarena; León Larios, Fátima; Corrales-Gutiérrez, Isabel; Universidad de Sevilla. Departamento de Cirugía; Universidad de Sevilla. Departamento de Enfermería; Universidad de Sevilla. HUM-873: Centro de Investigación y Acción Comunitaria de la Universidad de Sevilla; Universidad de Sevilla. CTS-607: Salud reproductiva de la mujer
According to the United Nations Population Fund, approximately every two minutes, a woman dies, totalling approximately 800 deaths per day for reasons related to a maternal causes. Therefore, within the Sustainable Development Goals of the 2030 Agenda, the goal is to reduce the global maternal mortality rate to less than 70 deaths per 100,000 live births by 2030. We aim to analyse strategies to reduce maternal mortality in the context of the Sustainable Development Goals, identify barriers that hinder their implementation, and analyse the impact of the COVID-19 pandemic on their achievement. We performed a scoping review of the following databases and distributors of specialised information in the health area: PubMed, Medes, Lilacs, Cuiden, Cinahl, Scopus, SciELO, and websites of the United Nations (UN), United Nations Population Fund (UNFPA), and Cooperanda. A total of 24 articles were reviewed. The results obtainded determined that reviewed studies agree that despite efforts to reduce maternal mortality, significant social and structural barriers still exist in developing countries that slow the implementation of strategies to protect maternal health, especially in the most at-risk populations, and the improvement of the health system. The main strategy consists of increasing the number of deliveries attended by qualified personnel, which is the greatest indicator of progress in reducing maternal mortality. To conclude, Economic, social, and political barriers remain in developing countries in the context of the Sustainable Development Goals. The intervention that has shown the most success is the attendance of deliveries by qualified personnel. It is essential to prioritise maternal care, triangular cooperation, and effective connection between the different Sustainable Development Goals.
CCAAT/Enhancer binding protein β induces motility and invasion of glioblastoma cells through transcriptional regulation of the calcium binding protein S100A4
(Impact Journals, 2015-01-24) Aguilar Morante, Diana; Morales-García, J. A.; Santos, A.; Pérez-Castillo, A.; Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica; Fundacion Mutua Madrilena; Instituto de Salud Carlos III; MINECO; Universidad de Sevilla. CTS-007: Fisiopatología de células madre neurales
We have previously shown that decreased expression of CCAAT/enhancer binding protein β (C/EBPβ) inhibits the growth of glioblastoma cells and diminishes their transformation capacity and migration. In agreement with this, we showed that C/EBPβ depletion decreases the mRNA levels of different genes involved in metastasis and invasion. Among these, we found S100 calcium binding protein A4 (S100A4) to be almost undetectable in glioblastoma cells deficient in C/EBPβ. Here, we have evaluated the possible role of S100A4 in the observed effects of C/EBPβ in glioblastoma cells and the mechanism through which S100A4 levels are controlled by C/EBPβ. Our results show that C/EBPβ suppression significantly reduced the levels of S100A4 in murine GL261 and human T98G glioblastoma cells. By employing an S100A4-promoter reporter, we observed a significant induction in the transcriptional activation of the S100A4 gene by C/EBPβ. Furthermore, overexpression of S100A4 in C/EBPβ-depleted glioblastoma cells reverses the enhanced migration and motility induced by this transcription factor. Our data also point to a role of S100A4 in glioblastoma cell invasion and suggest that the C/EBPβ gene controls the invasive potential of GL261 and T98G cells through direct regulation of S100A4. Finally, this study indicates a role of C/EBPβ on the maintenance of the stem cell population present in GL261 glioblastoma cells.
Neural crest derived progenitor cells contribute to tumor stroma and aggressiveness in stage 4/M neuroblastoma
(Impact Journals, 2017-09-21) Linares Clemente, Pedro; Aguilar Morante, Diana; Rodríguez Prieto, Ismael; Ramírez, Gema; Torres, Carmen de; Santamaría, Vicente; Pascual-Vaca, Diego; Colmenero-Repiso, Ana; Vega Moreno, Francisco Manuel; Pardal Redondo, Ricardo; Universidad de Sevilla. Departamento de Biología Celular; Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica; Asociacion Espanola contra el Cancer (AECC); European Research Council (ERC Starting Grant (CBSCs)); FEDER; Spanish Ministry of Economy and Competitiviness SAF; Universidad de Sevilla. CTS-007: Fisiopatología de células madre neurales
Pediatric tumors arise upon oncogenic transformation of stem/progenitor cells during embryonic development. Given this scenario, the existence of non-tumorigenic stem cells included within the aberrant tumoral niche, with a potential role in tumor biology, is an intriguing and unstudied possibility. Here, we describe the presence and function of non-tumorigenic neural crest-derived progenitor cells in aggressive neuroblastoma (NB) tumors. These cells differentiate into neural crest typical mesectodermal derivatives, giving rise to tumor stroma and promoting proliferation and tumor aggressiveness. Furthermore, an analysis of gene expression profiles in stage 4/M NB revealed a neural crest stem cell (NCSC) gene signature that was associated to stromal phenotype and high probability of relapse. Thus, this NCSC gene expression signature could be used in prognosis to improve stratification of stage 4/M NB tumors. Our results might facilitate the design of new therapies by targeting NCSCs and their contribution to tumor stroma.
Nursing students’ satisfaction with clinical simulation: a cross-sectional observational study
(MDPI, 2024-10-25) Jiménez-Álvarez, Juan Antonio; Guerra Martín, María Dolores; Borrallo Riego, Álvaro; Universidad de Sevilla. Departamento de Enfermería; Universidad de Sevilla. CTS-969: Innovacion en cuidados y determinantes sociales en salud
Clinical Simulation improves results in the students’ learning tests and allows for preserving acquired knowledge for longer periods of time, promoting more significant learning. This study was conducted to analyze Nursing students’ satisfaction with Clinical Simulation in three centres attached to a university from southern Spain. Methods: A quantitative, non-experimental and cross-sectional descriptive study was carried out. The students included were attending their third year of the Nursing undergraduate course and had already taken part in training sessions by means of Clinical Simulation. The Satisfaction Scale with High-Fidelity Clinical Simulation in Students (SSHF) was used for data collection. This scale has been validated and has 33 items grouped into eight factors. The SPSS software (version 28), was used for data analysis, establishing p-values < 0.05 for the statistically significant differences. Results: The participants were 180 students, with a mean age of 22.17 years old. Of them, 90.56% belonged to the female gender. A mean score of 3.82 out of 5 was obtained in the SSHF items. The items that obtained the highest scores were the following: benefits of Clinical Simulation as it relates theory with practise; possibility of learning based on the mistakes made; and comfort and respect while the sessions were developed. The item that obtained the lowest score was “timing for each simulation case”. We found significant differences in the results obtained according to each attached centre. Conclusions: The students showed high satisfaction levels regarding High-Fidelity Clinical Simulation in each of the three attached centres included in the study. Nevertheless, they stated the need to invest more time in Clinical Simulation sessions.
Priorities and challenges in social and healthcare policies for older people living in the Mediterranean basin: A Delphi panel study
(BMC, 2024-10-08) Allande Cussó, Regina; Porcel Gálvez, Ana María; Fernández García, Elena; Essawi, Salma; Salama, Mohamed; Universidad de Sevilla. Departamento de Enfermería; Universidad de Sevilla. CTS-1050: Cuidados Complejos, Cronicidad y Resultados en Salud; Universidad de Sevilla. CTS-1141: Investigación Clínica Aplicada a los Cuidados y Nuevos Paradigmas Asistenciales
Background: Recently, the countries in the Mediterranean basin (which share cultural ties) have been showing a common trend of declining social support for the elderly, with deficiencies in social care models for this demographic. Thus, this Delphi panel study analyzed the gaps in social and integrated care among the participating countries in a European research project. Methods: This three-round Delphi panel study focused on the European countries of Greece and Spain and the non-European countries of Egypt, Lebanon, and Tunisia. In this project, experts were identified according to their level of expertise in the subject matter, their gender, and their membership in different social groups. Subsequently, they were asked to identify the current situation of social care, set future goals, and locate the gaps regarding the social and healthcare models for older people in the participating countries. The median score (Mdn) and interquartile range (IQR) were calculated to assess the degree of consensus on the different priorities. Results: Among the participating countries, there was a lack of state agreements to maintain social care models, a lack of coordination between public and private institutions to provide social care services, territorial inequalities in terms of access and coverage of rights, and job insecurity for professionals. The desired situation was to integrate social and healthcare services with a person-centered social care model, thus promoting autonomy and empowering users and families in participation and decision-making. Conclusions: This Delphi study reveals significant disparities in social and healthcare policies for older adults across Mediterranean countries, highlighting shared challenges and specific national needs. European nations like Greece and Spain face fragmented systems, while non-European countries such as Lebanon, Egypt, and Tunisia lack specialized geriatric services and social security. All countries urgently need better professional training, social and economic empowerment of older adults, and integrated national strategies. These findings offer key insights for policymakers to develop equitable, sustainable solutions for aging populations.
Effects of adding electro-massage to manual therapy for the treatment of individuals with myofascial temporomandibular pain: a randomized controlled trial
(Universidade de São Paulo, 2024-09-16) Espejo-Antúnez, Luis; Cardero-Durán, María de Los Ángeles; Heredia Rizo, Alberto Marcos; Casuso-Holgado, María Jesús; Albornoz Cabello, Manuel; Universidad de Sevilla. Departamento de Fisioterapia
Objective: To evaluate the effect of the addition of dynamic cervical electrical stimulation (electro-massage, ES) to manual therapy (MT), compared to MT by itself, in individuals with myofascial temporomandibular pain. Methodology: A total of 46 participants with bilateral myofascial temporomandibular pain for at least three months were distributed into two groups. Group 1 (n=21) received local MT consisting of soft tissue mobilization and release techniques over the neck and temporomandibular regions. Group 2 (n=25) received an ES procedure in the cervical region combined with the same intervention as group 1. All participants underwent a 2-week protocol. The primary outcomes were pain intensity (Visual Analogue Scale), pressure pain threshold (PPT) at the masseter and upper trapezius muscles (algometer), and pain-free vertical mouth opening (manual gauge). The secondary outcome was active cervical range-of-movement. Measurements were taken at baseline, immediately after intervention, and at a 4-week follow-up. Results: The ANOVA revealed significant changes over group*time, with better results for group 2 (large effect sizes) regarding pain intensity (p< 0.001; η2>0.14), pressure pain sensitivity and mouth opening (p<0.001; η2>0.14). Similar findings were observed for active cervical range-of-movement in all directions (p<0.001; η2>0.14), except rotation (p≥0.05). Conclusion: Electrical stimulation therapy over the cervical region combined with a MT protocol over the neck and temporomandibular joint shows better clinical benefits than MT by itself in subjects with myofascial temporomandibular pain. Registration code: NCT04098952.
Mecanismos patogénicos en la fibrosis pulmonar idiopática. Implicación de los canales de Aquoporina 1
(Asociación de Neumólogos del Sur, 2019) Rodríguez Portal, José Antonio; Arellano Orden, Elena; Suárez Luna, Nela; Gómez Izquierdo, L.; Barrera Talavera, María Dolores; Echevarría Irusta, Miriam; Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica; Instituto de Biomedicina de Sevilla (IBIS); Universidad de Sevilla. CTS1047: Fisiopatología asociada a las Acuaporinas.
La fibrosis pulmonar idiopática (FPI) es una enfermedad pulmonar intersticial difusa (EPID) y progresiva. La FPI resulta de la alteración en la reepitelización tras la lesión de las células epiteliales alveolares. Se produce un aumento en la apoptosis epitelial y en la síntesis de mediadores pro fibróticos, con la consiguiente proliferación de fibroblastos, transformación a miofibrobastos y el depósito incontrolado de matriz extracelular. La aquoporina 1 (AQP1), es una proteína que facilita el movimiento de agua entre el espacio aéreo pulmonar y el parénquima y se ha demostrado que se regula al alza en animales expuestos a hipoxia. La AQP1 se expresa en células endoteliales, pero no en el epitelio alveolar pulmonar. Más recientemente se ha asociado a la AQP1 en los mecanismos implicados en la proliferación celular. Nuestro objetivo principal ha sido evaluar la participación de las Aquoporinas (AQPs) pulmonares en la patogenia de la FPI. Hemos analizado la expresión de AQP1 en biopsias de pacientes diagnosticados con FPI según los criterios de la ATS/ERS/ALAT de 2011 y en otras patologías pulmonares tales como la neumonitis por hipersensibilidad, sarcoidosis y biopsias de controles sanos. Los resultado de la inmunohistoquímica revelaron una intensa expresión de AQP1 en neumocitos tipo II hiperplásicos solo en las muestras obtenidas de pacientes con FPI. Además hay una inducción de la expresión de AQP1 (mRNA y proteína) tras la estimulación con TGF-β lo que acompaña a los cambios típicos del proceso de transición epitelio mesenquimal. Por lo tanto, la aparición de AQP1 en las células hiperplásicas tipo II y su regulación podrían estar implicadas en la patogénesis de la FPI.
Aquaporin-1 plays important role in proliferation by affecting cell cycle progression
(Wiley, 2016-06-16) Galán-Cobo, Ana; Ramírez Lorca, Reposo; Toledo Aral, Juan José; Echevarría Irusta, Miriam; Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica; Instituto de Biomedicina de Sevilla (IBIS)
Aquaporin-1 (AQP1) has been associated with tumor development. Here, we investigated how AQP1 may affect cell proliferation. The proliferative rate of adult carotid body (CB) cells, known to proliferate under chronic hypoxia, was analyzed in wild-type (AQP1 +/+) and knock out (AQP1 -/-) mice, maintained in normoxia or exposed to hypoxia while BrdU was administered. Fewer numbers of total BrdU+ and TH-BrdU+ cells were observed in AQP1 -/- mice, indicating a role for AQP1 in CB proliferation. Then, by flow cytometry, cell cycle state and proliferation of cells overexpressing AQP1 were compared to those of wild-type cells. In the AQP1-overexpressing cells, we observed higher cell proliferation and percentages of cells in phases S and G2/M and fewer apoptotic cells after nocodazole treatment were detected by annexin V staining. Also in these cells, proteomic assays showed higher expression of cyclin D1 and E1 and microarray analysis revealed changes in many cell proliferation-related molecules, including, Zeb 2, Jun, NF-kβ, Cxcl9, Cxcl10, TNF, and the TNF receptor. Overall, our results indicate that the presence of AQP1 modifies the expression of key cell cycle proteins apparently related to increases in cell proliferation. This contributes to explaining the presence of AQP1 in many different tumors.
Effects of haptic feedback interventions in post-stroke gait and balance disorders: a systematic review and meta-analysis
(MDPI, 2024) Gomez-Risquet, Maria; Cáceres Matos, Rocío; Magni, Eleonora; Luque Moreno, Carlos; Universidad de Sevilla. Departamento de Enfermería; Universidad de Sevilla. Departamento de Fisioterapia
Background: Haptic feedback is an established method to provide sensory information (tactile or kinesthetic) about the performance of an activity that an individual can not consciously detect. After a stroke, hemiparesis usually leads to gait and balance disorders, where haptic feedback can be a promising approach to promote recovery. The aim of the present study is to understand its potential effects on gait and balance impairments, both after interventions and in terms of immediate effects. Methods: This research was carried out using the following scientific databases: Embase, Scopus, Web of Science, and Medline/PubMed from inception to May 2024. The Checklist for Measuring quality, PEDro scale, and the Cochrane collaboration tool were used to assess the methodological quality and risk of bias of the studies. Results: Thirteen articles were chosen for qualitative analysis, with four providing data for the meta-analysis. The findings did not yield definitive evidence on the effectiveness of haptic feedback for treating balance and gait disorders following a stroke. Conclusions: Further research is necessary in order to determine the effectiveness of haptic feedback mechanisms, with larger sample sizes and more robust methodologies. Longer interventions and pre–post design in gait training with haptic feedback are necessary.
Rearrangement of cell types in the rat carotid body neurogenic niche induced by chronic intermittent hypoxia
(Wiley, 2023-01-17) Caballero Eraso, Candela; Colinas Miranda, Olalla; González Montelongo, Rafaela; Cabeza, Jose M.; Gao Chen, Lin; Pardal Redondo, Ricardo; López Barneo, José; Ortega Sáenz, Patricia; Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica; Instituto de Biomedicina de Sevilla (IBIS); European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Junta de Andalucía. Consejería de Salud; Ministerio de Ciencias, Innovación y Salud.
The carotid body (CB) is a prototypical acute oxygen (O2)-sensing organ that mediates reflex hyperventilation and increased cardiac output in response to hypoxaemia. CB overactivation, secondary to the repeated stimulation produced by the recurrent episodes of intermittent hypoxia, is believed to contribute to the pathogenesis of sympathetic hyperactivity present in sleep apnoea patients. Although CB functional plasticity induced by chronic intermittent hypoxia (CIH) has been demonstrated, the underlying mechanisms are not fully elucidated. Here, we show that CIH induces a small increase in CB volume and rearrangement of cell types in the CB, characterized by a mobilization of immature quiescent neuroblasts, which enter a process of differentiation into mature, O2-sensing and neuron-like, chemoreceptor glomus cells. Prospective isolation of individual cell classes has allowed us to show that maturation of CB neuroblasts is paralleled by an upregulation in the expression of specific glomus cell genes involved in acute O2-sensing. CIH enhances mitochondrial responsiveness to hypoxia in maturing neuroblasts as well as in glomus cells. These data provide novel perspectives on the pathogenesis of CB-mediated sympathetic overflow that may lead to the development of new pharmacological strategies of potential applicability in sleep apnoea patients.
Carotid body function in tyrosine hydroxylase conditional Olfr78 knockout mice
(Oxford University Press, 2024-02-22) Colinas Miranda, Olalla; Mombaerts, Peter; López Barneo, José; Ortega Sáenz, Patricia; Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica; Instituto de Biomedicina de Sevilla (IBIS); Ministerio de Ciencia e Innovación (MICIN). España
The Olfr78 gene encodes a G-protein-coupled olfactory receptor that is expressed in several ectopic sites. Olfr78 is one of the most abundant mRNA species in carotid body (CB) glomus cells. These cells are the prototypical oxygen (O2) sensitive arterial chemoreceptors, which, in response to lowered O2 tension (hypoxia), activate the respiratory centers to induce hyperventilation. It has been proposed that Olfr78 is a lactate receptor and that glomus cell activation by the increase in blood lactate mediates the hypoxic ventilatory response (HVR). However, this proposal has been challenged by several groups showing that Olfr78 is not a physiologically relevant lactate receptor and that the O2-based regulation of breathing is not affected in constitutive Olfr78 knockout mice. In another study, constitutive Olfr78 knockout mice were reported to have altered systemic and CB responses to mild hypoxia. To further characterize the functional role of Olfr78 in CB glomus cells, we here generated a conditional Olfr78 knockout mouse strain and then restricted the knockout to glomus cells and other catecholaminergic cells by crossing with a tyrosine hydroxylase-specific Cre driver strain (TH-Olfr78 KO mice). We find that TH-Olfr78 KO mice have a normal HVR. Interestingly, glomus cells of TH-Olfr78 KO mice exhibit molecular and electrophysiological alterations as well as a reduced dopamine content in secretory vesicles and neurosecretory activity. These functional characteristics resemble those of CB neuroblasts in wild-type mice. We suggest that, although Olfr78 is not essential for CB O2 sensing, activation of Olfr78-dependent pathways is required for maturation of glomus cells.
The bacteremia of dental origin and its implications in the appearance of bacterial endocarditis
(Medicina oral S.L, 2014) Mang-de la Rosa, María Rocío; Castellanos Cosano, Lizett; Romero-Pérez, María Jesús; Cutando, Antonio; Universidad de Sevilla. Departamento de Estomatología; Instituto de Biomedicina de Sevilla (IBIS)
Numerous systemic diseases may affect the oral cavity and vice versa,in particular severe diseases that involve the heart valve. In these cases, additional measures or a modification to our dental treatment need to be taken. We are aware of various diseases that can cause the emergence of bacterial endocarditis (BE), such as; rheumatic fever, valve lesions due to intravenous drug use, Kawasaki disease and valve surgery, among others. Due to its severity when it is not taken into account in dental treatment, we intend to show the evolution of the antimicrobial prophylaxis towards this condition. Furthermore, we intend to publish the current guidelines of institutions and societies which increasingly encourage rational antimicrobial use. In addition, we intend to examine the evidence of the possible origins of this disease during dental treatment and at the same time describe the necessary considerations that need to be taken during dental treatment.
Postoperative pain after one-visit root-canal treatment on teeth with vital pulps: Comparison of three different obturation techniques
(Medicina oral S.L, 2012) Alonso-Ezpeleta, Luis O.; Gasco-García, Carmen; Castellanos Cosano, Lizett; Martín González, Jenifer; López Frías, Francisco Javier; Segura Egea, Juan José; Universidad de Sevilla. Departamento de Estomatología; Instituto de Biomedicina de Sevilla (IBIS)
OBJECTIVES: To investigate and compare postoperative pain after one‐visit root canal treatment (RCT) on teeth with vital pulps using three different obturation techniques. STUDY DESIGN: Two hundred and four patients (105 men and 99 women) aged 12 to 77 years were randomly assigned into three treatments groups: cold lateral compaction of gutta‐percha (LC), Thermafil technique (TT), and Backfill ‐ Thermafil obturation technique (BT). Postoperative pain was recorded on a visual analogue scale (VAS) of 0 ‐ 10 after 2 and 6 hours, and 1, 2, 3, 4, 5, 6 and 7 days. Data were statistically analyzed using multivariate logistic regression analysis. RESULTS: In the total sample, 87% of patients experienced discomfort or pain in some moment between RCT and the seventh day. The discomfort experienced was weak, light, moderate and intense in 6%, 44%, 20% and 6% of the cases, respectively. Mean pain levels were 0.4 ± 0.4, 0.4 ± 0.3, and 1.4 ± 0.7 in LC, BT, and TT groups, respectively. Patients of TT group experienced a significantly higher mean pain level compared to other two groups (p < 0.0001). In TT group, all patients felt some level of pain at six hours after RCT. CONCLUSIONS: Postoperative pain was significantly associated with the obturation technique used during root canal treatment. Patients whose teeth were filled with Thermafil obturators (TT technique) showed significantly higher levels of discomfort than patients whose teeth were filled using any of the other two techniques.
Influence of root canal instrumentation and obturation techniques on intra-operative pain during endodontic therapy
(Medicina oral S.L, 2012) Martín González, Jenifer; Echevarría-Pérez, Marta; Sánchez Domínguez, Benito; Tarilonte Delgado, María Luisa; Castellanos Cosano, Lizett; López Frías, Francisco Javier; Segura Egea, Juan José; Universidad de Sevilla. Departamento de Estomatología; Instituto de Biomedicina de Sevilla (IBIS)
Objective: To analyse the influence of root canal instrumentation and obturation techniques on intra-operative pain experienced by patients during endodontic therapy. Method and Materials: A descriptive cross-sectional study was carried out in Ponferrada and Sevilla, Spain, including 80 patients (46 men and 34 women), with ages ranged from 10 to 74 years, randomly recruited. Patient gender and age, affected tooth, pulpal diagnosis, periapical status, previous NSAID or antibiotic (AB) treatment, and root canal instrumentation and obturation techniques were recorded. After root canal treatment (RCT), pa tients completed a 10-cm visual analogue scale (VAS) that ranked the level of pain. Results were analysed statisti cally using the Chi-square and ANOVA tests and logistic regression analysis. Results: The mean pain level during root canal treatment was 2.9 ± 3.0 (median = 2) in a VAS between 0 and 10. Forty percent of patients experienced no pain. Gender, age, arch, previous NSAIDs or AB treatment and anaesthetic type did not influence significantly the pain level (p > 0.05). Pain during root canal treatment was significantly greater in molar teeth (OR = 10.1; 95% C.I. = 1.6 - 63.5; p = 0.013). Root canal instrumentation and obturation techniques did not affect significantly patient’s pain during root canal treatment (p > 0.05). Conclusion: Patients feel more pain when RCT is carried out on molar teeth. The root canal instrumentation and obturation techniques do not affect significantly the patients’ pain during RCT.
External apical root resorption in maxillary root-filled incisors after orthodontic treatment: A split-mouth design study
(Medicina oral S.L, 2012) Llamas Carreras, José María; Amarilla, Almudena; Espinar-Escalona, E.; Castellanos Cosano, Lizett; Martín González, Jenifer; Sánchez Domínguez, Benito; López Frías, Francisco Javier; Universidad de Sevilla. Departamento de Estomatología; Instituto de Biomedicina de Sevilla (IBIS)
Introduction: The purpose of this study was to compare, in a split mouth design, the external apical root resorption (EARR) associated with orthodontic treatment in root-filled maxillary incisors and their contralateral teeth with vital pulps. Methodology: The study sample consisted of 38 patients (14 males and 24 females), who had one root-filled incisor before completion of multiband/bracket orthodontic therapy for at least 1 year. For each patient, digital panoramic radiographs taken before and after orthodontic treatment were used to determine the root resortion and the pro portion of external root resorption (PRR), defined as the ratio between the root resorption in the endodontically treated incisor and that in its contralateral incisor with a vital pulp. The student’s t-test, chi-square test and logistic regression analysis were used to determine statistical significance. Results: There was no statistically significant difference (p > 0.05) between EARR in vital teeth (1.1 ± 1.0 mm) and endodontically treated incisors (1.1 ± 0.8 mm). Twenty-six patients (68.4%) showed greater resorption of the endodontically treated incisor than its homolog vital tooth (p > 0.05). The mean and standard deviation of PPR were 1.0 ± 0.2. Multivariate logistic regression suggested that PRR does not correlate with any of the variables analyzed. Conclusions: There was no significant difference in the amount or severity of external root resorption during or thodontic movement between root-filled incisors and their contralateral teeth with vital pulps.
Impact of rare diseases in oral health
(Medicina oral S.L, 2016) Molina García, Ana; Castellanos Cosano, Lizett; Machuca-Portillo, Guillermo; Posada de la Paz, Manuel; Universidad de Sevilla. Departamento de Estomatología; Instituto de Biomedicina de Sevilla (IBIS); Universidad de Sevilla. CTS1000: Odontología en pacientes especiales, médicamente comprometidos y hospitalaria.
Background: Rare diseases (RD) are those that present a lower prevalence than 5 cases per 10.000 population. The main objective of this review was to study the effect on oral health in rare diseases, while the secondary objective of the study is theme upgrade. Material and Methods: Comparative observational case-control studies were analysed and a systematic review was conducted in PubMed. Each rare disease listed on the statistical data record of the Health Portal of the Ministry of Equality, Health and Social Policies Board of Andalusia was associated with “oral health”. The variables studied included dental, oral mucosa and occlusion alterations, oral pathologies (caries, periodontal disease) and other alterations (mouth breathing, parafunctional habits, etc). A bias analysis of the variable caries was conducted. Results: Six RD were selected through our inclusion and exclusion criteria (hypogammaglobulinemia, Rett syn drome, Marfan syndrome, Prader-Willi syndrome, cystic fibrosis and Cri du chat syndrome) in a total of 8 publi cations, of which four trials were classified as high risk of bias and one of them as medium risk. There were not trials with low risk of bias. Conclusions: The main statistically significant differences found by Syndrome compared to a control group were in Hypogammaglobulinemia with a greater tendency to enamel hypoplasia and dry mouth. The Rett syndrome had, as well, a greater tendency to an anterior open bite, ogival palate, bruxism, mouth breathing and tongue thrusting. Prader-Willi syndrome had a tendency of dental erosion, and Cri du chat syndrome showed a higher association to Tannerella forsythia.
2-years retrospective observational case-control study on survival and marginal bone loss of implants in patients with hereditary coagulopathies
(Medicina oral S.L, 2023) Pérez Fierro, Manuel Luis; Castellanos Cosano, Lizett; Hueto-Madrid, Juan Antonio; López Jiménez, Julián; Núñez Vázquez, Ramiro José; Machuca-Portillo, Guillermo; Universidad de Sevilla. Departamento de Estomatología; Instituto de Biomedicina de Sevilla (IBIS)
Background: Evaluating 2-years implant loss and marginal bone loss in patients with hereditary coagulopathies, comparing with a healthy control group. Material and Methods: 37 implants in 13 patients (17 haemophilia A, 20 Von-Willebrand disease) versus 26 im plants in 13 healthy patients. Data measured through Lagervall-Jansson index (after surgery, at prosthetic loading, at 2 years). Statistics: Chi-square, Haberman’s, ANOVA, Mann-Whitney-U. Significance p<0.05. Results: Haemorrhagic accidents in 2 coagulopathies patients (non-statistical differences). Hereditary coagulopa thies patients suffered more hepatitis (p<0.05), HIV (p<0.05) and less previous periodontitis (p<0.01). Non-sta tistical differences in marginal bone loss among groups. 2 implants were lost in the hereditary coagulopathies and none in the control group (non-statistical differences). Hereditary coagulopathies patients had longer (p<0.001), and narrower implants (p<0.05) placed. 43.2% external prosthetic connection in hereditary coagulopathies pa tients (p<0.001); change of prosthetic platform more frequent in control group (p<0.05). 2 implants lost: external connection (p<0.05). Survival rate 96.8% (hereditary coagulopathies 94.6%, control group 100%). Conclusions: Implant and marginal bone loss at 2 years is similar in patients with hereditary coagulopathies and control group. Precautions should be taken on the treatment for hereditary coagulopathies patients, through prior haematological protocol. Implant loss only occurred in in a patient with Von-Willebrand´s disease.

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