Artículos (Ingeniería Química)

URI permanente para esta colecciónhttps://hdl.handle.net/11441/53665

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Applied rheology as tool for the assessment of chitosan hydrogels for regenerative medicine
(Multidisciplinary Digital Publishing Institute (MDPI), 2021) Sánchez-Cid Bueno, Pablo; Jiménez-Rosado, Mercedes; Alonso González, María; Romero García, Alberto; Pérez-Puyana, Víctor Manuel; Universidad de Sevilla. Departamento de Ingeniería Química
The regeneration of soft tissues that connect, support or surround other tissues is of great interest. In this sense, hydrogels have great potential as scaffolds for their regeneration. Among the different raw materials, chitosan stands out for being highly biocompatible, which, together with its biodegradability and structure, makes it a great alternative for the manufacture of hydrogels. Therefore, the aim of this work was to develop and characterize chitosan hydrogels. To this end, the most important parameters of their processing, i.e., agitation time, pH, gelation temperature and concentration of the biopolymer used were rheologically evaluated. The results show that the agitation time does not have a significant influence on hydrogels, whereas a change in pH (from 3.2 to 7) is a key factor for their formation. Furthermore, a low gelation temperature (4◦C) favors the formation of the hydrogel, showing better mechanical properties. Finally, there is a percentage of biopolymer saturation, from which the properties of the hydrogels are not further improved (1.5 wt.%). This work addresses the development of hydrogels with high thermal resistance, which allows their use as scaffolds without damaging their mechanical properties.
Assessment of the Processing Method for the Development of Hybrid Biopolymer-Based Scaffolds
(LIDSEN Publishing Inc, 2020-02-26) Alonso González, María; Rubio Valle, José Fernando; Pérez-Puyana, Víctor Manuel; Jiménez-Rosado, Mercedes; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Economía y Competitividad (MINECO). España; European Union (UE)
The present study deals with the development of scaffolds based on gelatin (G) and/or chitosan (CH) through modifications in the central processing method. This process consists of the fabrication of a hydrogel which is, then submitted to a freeze-drying stage. To compare the effects of the different modifications, the mechanical properties of the various systems were characterized, employing both dynamic compressive strain and frequency sweep tests. In addition, their porosity as well as the structure and fiber distribution, using an analytic model and scanning electron microscopy (SEM), were also evaluated. The obtained results demonstrated a strong dependence on the properties by the scaffolds with both the modifications introduced in the processing method as well as the proportion of materials used (G and CH). Furthermore, the properties were found to improve for systems with a high chitosan content after being submitted to a heat treatment at 50 °C with agitation.
Desarrollo de matrices bioplásticas superabsorbentes de proteína de soja con micronutriente incorporado para su uso en horticultura
(Asociación de Químicos e Ingenieros del Instituto Químico de Sarriá, 2019) Jiménez-Rosado, Mercedes; Alonso González, María; Aguilar García, José Manuel; Cordobés Carmona, Felipe; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
El aumento en el consumo de productos orgánicos procedentes de la horticultura sostenible sumado a la preocupación por los efectos de la sequía, cada vez más pronunciada, ha incrementado la necesidad de buscar nuevas técnicas que ayuden a la minimización del impacto ambiental. De esta forma, se ha investigado la elaboración de matrices proteicas superabsor-bentes con micronutrientes, pudiendo así retener agua del medio para suministrarla a la planta junto con los micronutrientes de forma controlada. No obstante, la incorporación a las matrices de las sales generalmente usadas (sulfatos) hacen que éstas pierdan su capacidad superabsorbente, por lo que hay que buscar otra forma de incorporar estos micronutrientes. El objetivo principal del presente trabajo es la evaluación de la incorporación de diferentes sales (sulfato e hidroxi-carbonato), las cuales crean diferentes fuerzas iónicas en el medio a absorber. De esta forma, se compara el efecto de la fuerza iónica en la capacidad superabsorbente de las matrices. Así, se han obtenido matrices proteicas de soja con diferentes concentraciones de sales, comprobando que la incorporación de estas a las matrices conduce a peores propiedades mecánicas. Además, se ha comprobado que la incorporación de una sal que crea una fuerza iónica débil (hidroxicar-bonato) hace que las matrices tengan una mejor capacidad de absorción, mientras que la incorporación de una sal con un alto potencial a generar fuerzas iónicas (sulfato) hace que estos materiales pierdan su capacidad superabsorbente.
Development of rice bran-based bioplastics via injection molding: Influence of particle size and glycerol ratio
(Elsevier, 2024) Alonso González, María; Félix Ángel, Manuel; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Ciencia e Innovación (MICIN). España; Universidad de Sevilla
Plastics play a vital role in modern society but their non-biodegradable nature has led to environmental concerns. Biomass-derived biodegradable bioplastics offer an eco-friendly alternative and protein and starch-based bioplastics, sourced from agro-food residues, are gaining prominence due to their renewability. However, many bio-based materials face challenges and developing efficient processing methods is crucial for their industrial viability. Optimizing particle size and plasticizer proportion is vital to tailor the properties of bioplastics. This study evaluates rice bran-based bioplastics, produced via injection molding, considering particle size and the impact of glycerol ratio. Smaller particle sizes enhance interactions during processing and, the conditions achieved during mixing determined the relevance of biopolymer–plasticizer and biopolymer–biopolymer interactions, leading to different behaviors depending on their balance. The processability and final properties of the materials developed were also affected by the glycerol ratio, with higher rice bran proportion leading to better rheological and mechanical properties.
Effect of different crosslinking agents on hybrid chitosan/collagen hydrogels for potential tissue engineering applications
(Elsevier, 2024) Sánchez Cid, Pablo; Alonso González, María; Jiménez-Rosado, Mercedes; Rafii-El-Idrissi Benhnia, Mohammed; Ruiz Mateos, Ezequiel; Ostos Marcos, Francisco José; Romero García, Alberto; Pérez-Puyana, Víctor Manuel; Universidad de Sevilla. Departamento de Ingeniería Química; Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología; Ministerio de Ciencia e Innovación (MICIN). España; Agencia Estatal de Investigación. España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Tissue engineering (TE) demands scaffolds that have the necessary resistance to withstand the mechanical stresses once implanted in our body, as well as excellent biocompatibility. Hydrogels are postulated as interesting materials for this purpose, especially those made from biopolymers. In this study, the microstructure and rheological performance, as well as functional and biological properties of chitosan and collagen hydrogels (CH/CG) crosslinked with different coupling agents, both natural such as D-Fructose (F), genipin (G) and transglutaminase (T) and synthetic, using a combination of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride with N-hydroxysuccinimide (EDC/NHS) will be assessed. FTIR tests were carried out to determine if the proposed crosslinking reactions for each crosslinking agent occurred as expected, obtaining positive results in this aspect. Regarding the characterization of the properties of each system, two main trends were observed, from which it could be established that crosslinking with G and EDC-NHS turned out to be more effective and beneficial than with the other two crosslinking agents, producing significant improvements with respect to the base CH/CG hydrogel. In addition, in vitro tests demonstrated the potential application in TE of these systems, especially for those crosslinked with G, T and EDC-NHS.
Effect of different crosslinking agents on hybrid chitosan/collagen hydrogels for potential tissue engineering applications
(Elsevier, 2024-04) Sánchez Cid, Pablo; Alonso González, María; Jiménez-Rosado, Mercedes; Rafii-El-Idrissi Benhnia, Mohammed; Ruiz-Mateos Carmona, Ezequiel; Ostos Marcos, Francisco José; Romero García, Alberto; Pérez-Puyana, Víctor Manuel; Universidad de Sevilla. Departamento de Ingeniería Química; Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología; Ministerio de Ciencia e Innovación (MICIN). España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Junta de Andalucía; Consejo Superior de Investigaciones Científicas (CSIC); Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes; Universidad de Sevilla. CTS590: Inmunovirología (Sistema Sanitario Público de Andalucía. Fundación Pública Andaluza para la Gestión de la Investigación en Salud de Sevilla (FISEVI)); Universidad de Sevilla. FQM206: Grupo de Cinética del Profesor Rodríguez Velasco
Tissue engineering (TE) demands scaffolds that have the necessary resistance to withstand the mechanical stresses once implanted in our body, as well as excellent biocompatibility. Hydrogels are postulated as interesting materials for this purpose, especially those made from biopolymers. In this study, the microstructure and rheological performance, as well as functional and biological properties of chitosan and collagen hydrogels (CH/CG) crosslinked with different coupling agents, both natural such as d-Fructose (F), genipin (G) and transglutaminase (T) and synthetic, using a combination of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride with N-hydroxysuccinimide (EDC/NHS) will be assessed. FTIR tests were carried out to determine if the proposed crosslinking reactions for each crosslinking agent occurred as expected, obtaining positive results in this aspect. Regarding the characterization of the properties of each system, two main trends were observed, from which it could be established that crosslinking with G and EDC-NHS turned out to be more effective and beneficial than with the other two crosslinking agents, producing significant improvements with respect to the base CH/CG hydrogel. In addition, in vitro tests demonstrated the potential application in TE of these systems, especially for those crosslinked with G, T and EDC-NHS.
Effects of Mould Temperature on Rice Bran-Based Bioplastics Obtained by Injection Moulding
(MDPI, 2021-01) Alonso González, María; Félix Ángel, Manuel; Guerrero Conejo, Antonio Francisco; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
The high production rate of conventional plastics and their low degradability result in severe environmental problems, such as plastic accumulation and some other related consequences. One alternative to these materials is the production of oil-free bioplastics, based on wastes from the agro-food industry, which are biodegradable. Not only is rice bran an abundant and non-expensive waste, but it is also attractive due to its high protein and starch content, which can be used as macromolecules for bioplastic production. The objective of this work was to develop rice-bran-based bioplastics by injection moulding. For this purpose, this raw material was mixed with a plasticizer (glycerol), analysing the effect of three mould temperatures (100, 130 and 150 °C) on the mechanical and microstructural properties and water absorption capacity of the final matrices. The obtained results show that rice bran is a suitable raw material for the development of bioplastics whose properties are strongly influenced by the processing conditions. Thus, higher temperatures produce stiffer and more resistant materials (Young’s modulus improves from 12 ± 7 MPa to 23 ± 6 and 33 ± 6 MPa when the temperature increases from 100 to 130 and 150 °C, respectively); however, these materials are highly compact and, consequently, their water absorption capacity diminishes. On the other hand, although lower mould temperatures lead to materials with lower mechanical properties, they exhibit a less compact structure, resulting in enhanced water absorption capacity.
Influence of Natural Crosslinkers on Chitosan Hydrogels for Potential Biomedical Applications
(Wiley, 2023-09) Sánchez Cid, Pablo; Gónzalez-Ulloa, Gabriel; Alonso González, María; Jiménez-Rosado, Mercedes; Rafii-El-Idrissi Benhnia, Mohammed; Romero García, Alberto; Ostos Marcos, Francisco José; Pérez-Puyana, Víctor Manuel; Universidad de Sevilla. Departamento de Ingeniería Química; Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología; Ministerio de Ciencia e Innovación (MICIN). España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Junta de Andalucía; European Commission. Fondo Social Europeo (FSO); Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes; Universidad de Sevilla. CTS590: Inmunovirología (Sistema Sanitario Público de Andalucía. Fundación Pública Andaluza para la Gestión de la Investigación en Salud de Sevilla (FISEVI)); Universidad de Sevilla. FQM206: Grupo de Cinética del Profesor Rodríguez Velasco
Chitosan (CH) is a very well-known biopolymer that has been widely used for the development of biomaterials with a wide range of applications in the biomedical field, such as the preparation of hydrogels, owing to its outstanding anti-inflammatory, antibacterial and antifungal properties, biocompatibility and biodegradability, although they present limited mechanical properties. Chemical crosslinking is one of the most recurrent strategies for the reinforcement of these structures and, above all, crosslinking with natural-origin compounds that do not compromise their biocompatibility is considered a hot topic in this research field. D-fructose (F), obtained from the hydrolyzation and further isomerization of starch, an abundant raw material and genipin (G), which is extracted from the fruits of Gardenia jasminoides Ellis are used as natural crosslinkers. Chitosan-based hydrogels crosslinked with each crosslinking agent are prepared and characterized through Fourier transform infrared (FTIR) spectroscopy, crosslinking and swelling degree determination, rheological, microstructural, and biological studies. The results demonstrate that crosslinking with G is more beneficial for chitosan-based hydrogels since these samples showed more compact structures and better rheological performance. Additionally, excellent biological in vitro behavior due to the crosslinking with G, unlike that of F.
Influence of the plasticizer on rice bran-based eco-friendly bioplastics obtained by injection moulding
(Elsevier, 2022) Alonso González, María; Félix Ángel, Manuel; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Ciencia e Innovación (MICIN). España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
The manufacture of eco-friendly bioplastic materials from renewable resources to replace petroleum-based plastics has attracted increasing attention. For many years, proteins, lipids and polysaccharides have been proposed as natural biopolymers sources to obtain biodegradable plastic materials. As by-product from the rice industry, rice bran, is an available and non-expensive resource of both proteins and starches, food groups that, properly processed, can be employed in the development of bioplastics. Plasticizers are essential for the manufacture of bioplastics and, when carbohydrate/protein mixtures are used, an adequate selection of plasticizers must be addressed. By these means, a material suitable for thermo-mechanical processing methods is obtained if starches are subjected to shear forces under high temperatures and water excess (gelatinisation). Moreover, additional polyol-based plasticizers, such as glycerol and sorbitol, allow obtaining reinforced products with improved elasticity when protein-based bioplastics are processed. The aim of the present study was to analyse the plasticizing effect of water combined with different proportions of glycerol or sorbitol, as well as their influence on the final bioplastic properties. Results indicate that higher water ratios produce stiffer bioplastics with improved viscoelastic moduli, maximum stress and Young´s modulus, while increasing the glycerol and sorbitol content leads to higher elasticities and water uptake capacities in general. Moreover, sorbitol seems to provide more suitable bioplastics with better tensile (up to 500% in Young´s modulus) and functional properties compared to glycerol.
Rice Bran Valorization through the Fabrication of Nanofibrous Membranes by Electrospinning
(MDPI, 2024) Alonso González, María; Félix Ángel, Manuel; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Ciencia e Innovación (MICIN). España; Universidad de Sevilla
The high production rate of fossil-based plastics, coupled with their accumulation and low degradability, is causing severe environmental problems. As a result, there is a growing interest in the use of renewable and natural sources in the polymer industry. Specifically, rice bran is a highly abundant by-product of the agro-food industry, with variable amounts of protein and starch within its composition, which are usually employed for bioplastic development. This study aims to valorize rice bran through the production of nanofiber membranes processed via electrospinning. Due to its low solubility, the co-electrospinning processing of rice bran with potato starch, known for its ability to form nanofibers through this technique, was chosen. Several fiber membranes were fabricated with modifications in solution conditions and electrospinning parameters to analyze their effects on the synthesized fiber morphology. This analysis involved obtaining micrographs of the fibers through scanning electron microscopy (SEM) and fiber diameter analysis. Potato starch membranes were initially investigated, and once optimal electrospinning conditions were identified, the co-electrospinning of rice bran and potato starch was conducted. Attempts were made to correlate the physical properties of the solutions, such as conductivity and density, with the characteristics of the resulting electrospun fibers. The results presented in this study demonstrate the potential valorization of a rice by-product for the development of bio-based nanofibrous membranes. This not only offers a solution to combat current plastic waste accumulation but also opens up a wide range of applications from filtration to biomedical devices (i.e., in tissue engineering).
Rice Bran-Based Bioplastics: Effects of Biopolymer Fractions on Their Mechanical, Functional and Microstructural Properties
(Multidisciplinary Digital Publishing Institute (MDPI), 2022) Alonso González, María; Félix Ángel, Manuel; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Agencia Estatal de Investigación. España RTI2018-097100-B-C21
Rice bran is an underutilized by-product of rice production, containing proteins, lipids and carbohydrates (mainly starches). Proteins and starches have been previously used to produce rice bran-based bioplastics, providing a high-added-value by-product, while contributing to the development of biobased, biodegradable bioplastics. However, rice bran contains oil (18–22%), which can have a detrimental effect on bioplastic properties. Its extraction could be convenient, since rice bran oil is becoming increasingly attractive due to its variety of applications in the food, pharmacy and cosmetic industries. In this way, the aim of this work was to analyze the effect of the different components of rice bran on the final properties of the bioplastics. Rice bran refining was carried out by extracting the oil and fiber fractions, and the effects of these two procedures on the final properties were addressed with mechanical, functional and microstructural measures. Results revealed that defatted rice bran produced bioplastics with higher viscoelastic moduli and better tensile behavior while decreasing the water uptake capacity and the soluble matter loss of the samples. However, no significant improvements were observed for systems produced from fiber-free rice bran. The microstructures observed in the SEM micrographs matched the obtained results, supporting the conclusions drawn.
Rice bran-based bioplastics: Effects of the mixing temperature on starch plastification and final properties
(Elsevier, 2021-10) Alonso González, María; Félix Ángel, Manuel; Guerrero Conejo, Antonio Francisco; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Ministerio de Ciencia e Innovación (MICIN). España; Agencia Estatal de Investigación. España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
The agro-food industry produces huge amounts of wastes and by-products with high levels of carbohydrates and proteins, basic food groups that, properly treated, can be employed for the development of bioplastics. These high added-value products represent an alternative to traditional polymers. In this research work, rice bran was mixed with glycerol and water obtaining homogeneous blends which then are processed into bioplastics via injection moulding. The mixing temperature aids starch plastification and thus, affects the properties of the final specimens. In this way, the mechanical characterization revealed improvements for the highest temperature (110 °C) used which, at the same time, exhibited poor physical integrity during water immersion. Although the mechanical properties of the dried system obtained at 80 °C are slightly inferior to those obtained for the non-dried 110 °C system, these specimens are considered more adequate since they exhibited higher physical integrity and, consequently, better operating conditions.
Valorization of Honduran agro-food waste to produce bioplastics
(MDPI, 2023-06) Castro-Criado, Daniel; Rivera-Flores, Octavio; Abdullah, Johar Amin Ahmed; Castro-Osorto, Elia; Alonso González, María; Ramos-Casco, Lucy; Pérez-Puyana, Víctor Manuel; Sánchez-Barahona, Marlon; Sánchez Cid, Pablo; Jiménez-Rosado, Mercedes; Romero García, Alberto; Universidad de Sevilla. Departamento de Ingeniería Química; Universidad de Sevilla. Departamento de Ingeniería Química y Ambiental; Ministerio de Ciencia e Innovación (MICIN). España; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Agencia Española de Cooperación Internacional para el Desarrollo (AECID); European Commission. Fondo Social Europeo (FSO); Universidad de Sevilla. TEP229: Tecnología y Diseño de Productos Multicomponentes
The development of biodegradable plastics and eco-friendly biomaterials derived from renewable resources is crucial for reducing environmental damage. Agro-industrial waste and rejected food can be polymerized into bioplastics, offering a sustainable solution. Bioplastics find use in various industries, including for food, cosmetics, and the biomedical sector. This research investigated the fabrication and characterization of bioplastics using three types of Honduran agro-wastes: taro, yucca, and banana. The agro-wastes were stabilized and characterized (physicochemically and thermically). Taro flour presented the highest protein content (around 4.7%) and banana flour showed the highest moisture content (around 2%). Furthermore, bioplastics were produced and characterized (mechanically and functionally). Banana bioplastics had the best mechanical properties, with a Young’s modulus around 300 MPa, while taro bioplastics had the highest water-uptake capacity (200%). In general, the results showed the potential of these Honduran agro-wastes for producing bioplastics with different characteristics that could add value to these wastes, promoting the circular economy.

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Examinando Artículos (Ingeniería Química) por Autor "Alonso González, María"