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A combinatorial strategy of alternative promoter use during differentiation of a heterocystous cyanobacterium


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dc.creator Muro Pastor, Alicia María es
dc.creator Brenes Álvarez, Manuel es
dc.creator Vioque Peña, Agustín es 2019-03-18T17:57:02Z 2019-03-18T17:57:02Z 2017-06-06
dc.identifier.citation Muro Pastor, A.M., Brenes Álvarez, M. y Vioque Peña, A. (2017). A combinatorial strategy of alternative promoter use during differentiation of a heterocystous cyanobacterium. Environmental Microbiology Reports, 9 (4), 449-458.
dc.identifier.issn 1758-2229 es
dc.description.abstract Heterocystous cyanobacteria such as Nostoc sp. are filamentous photosynthetic organisms that, in response to nitrogen deficiency, undergo a differentiation process transforming certain, semi-regularly spaced cells into heterocysts, devoted to nitrogen fixation. During transition to a nitrogen-fixing regime, growth of most vegetative cells in the filament is temporarily arrested due to nutritional deprivation, but developing heterocysts require intense transcriptional activity. Therefore, the coexistence of arrested vegetative cells and actively developing prospective heterocysts relies on the simultaneous operation of somewhat opposite transcriptional programs. We have identified genes with multiple nitrogen-responsive transcriptional starts appearing in seemingly paradoxical combinations. For instance, sigA, encoding the RNA polymerase housekeeping sigma factor, is transcribed from one major nitrogen stress-repressed promoter and from a second, nitrogen stress-induced promoter. Here, we show that both promoters are expressed with complementary temporal dynamics. Using a gfp reporter we also show that transcription from the inducible promoter takes place exclusively in differentiating heterocysts and is already detected before any morphological or fluorescence signature of differentiation is observed. Tandem promoters with opposite dynamics could operate a compensatory mechanism in which repression of transcription from the major promoter operative in vegetative cells is offset by transcription from a new promoter only in developing heterocyst. es
dc.description.sponsorship Ministerio de Economía y Competitividad BFU2013-48282-C2-1 es
dc.description.sponsorship Agencia Estatal de Investigación (AEI) BFU2016-74943-C2-1-P es
dc.description.sponsorship Ministerio de Educación, Cultura y Deportes FPU014/05123 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Wiley es
dc.relation.ispartof Environmental Microbiology Reports, 9 (4), 449-458.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri *
dc.title A combinatorial strategy of alternative promoter use during differentiation of a heterocystous cyanobacterium es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/submittedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular es
dc.relation.projectID BFU2013-48282-C2-1 es
dc.relation.projectID BFU2016-74943-C2-1-P es
dc.relation.projectID FPU014/05123 es
dc.relation.publisherversion es
dc.identifier.doi 10.1111/1758-2229.12555 es
idus.format.extent 29 p. es
dc.journaltitle Environmental Microbiology Reports es
dc.publication.volumen 9 es
dc.publication.issue 4 es
dc.publication.initialPage 449 es
dc.publication.endPage 458 es
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