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Cholesterol biosensing with a polydopamine-modified nanostructured platinum electrode prepared by oblique angle physical vacuum deposition

 

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dc.creator Martín, Mirian es
dc.creator Álvarez Molina, Rafael es
dc.creator Palmero Acebedo, Alberto es
dc.creator López Santos, Carmen es
dc.creator González Mora, José Luis es
dc.creator Salazar, Pedro es
dc.creator Rodríguez González-Elipe, Agustín es
dc.date.accessioned 2018-11-13T15:33:24Z
dc.date.available 2018-11-13T15:33:24Z
dc.date.issued 2017
dc.identifier.citation Martín, M., Álvarez Molina, R., Palmero Acebedo, A., López Santos, C., González Mora, J.L., Salazar, P. y Rodríguez González-Elipe, A. (2017). Cholesterol biosensing with a polydopamine-modified nanostructured platinum electrode prepared by oblique angle physical vacuum deposition. Sensors and Actuators - B - Chemical Biochemical Sensors, 240, 37-45.
dc.identifier.citation Martín, M., Álvarez Molina, R., Palmero Acebedo, A., López Santos, C., González Mora, J.L., Salazar, P. y Rodríguez González-Elipe, A. (2017). Cholesterol biosensing with a polydopamine-modified nanostructured platinum electrode prepared by oblique angle physical vacuum deposition. Sensors and Actuators - B - Chemical Biochemical Sensors, 240, 37-45.
dc.identifier.issn 0925-4005 es
dc.identifier.uri https://hdl.handle.net/11441/80117
dc.description.abstract This paper reports a novel cholesterol biosensor based on nanostructured platinum (Pt) thin films prepared by Magnetron Sputtering (MS) in an oblique angle (OAD) configuration. Pt thin films were deposited onto a gold screen-printed electrode and characterized using Rutherford Back Scattering (RBS), Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), Cyclic Voltammetry (CV), X-ray Photo-electron Spectroscopy (XPS), Atomic Force Microscopy (AFM) and wetting analysis. Our results confirmed that the film is highly porous and formed by tilted nanocolumns, with an inclination of around 40° and a total thickness of 280 nm. XRD and CV analysis confirmed the polycrystalline nature of the Pt thin film. Cholesterol oxidase (ChOx) was covalently immobilized using a bioinspired polymer, polydopamine (PDA), via Schiff base formation and Michael-type addition. After being immobilized, ChOx displayed apparent activation energy of 34.09 kJ mol−1 and Michaelis constant (KM) values of 34.09 kJ mol−1 and 3.65 mM, respectively, confirming the high affinity between ChOx and cholesterol and the excellent ability of the PDA film for immobilizing biological material without degradation. Under optimized working conditions the developed biosensor presented a sensitivity of 14.3 mA M−1cm−2 (R2:0.999) with a linear range up to 0.5 mM and a limit of detection of 10.5 μM (S/N = 3). Furthermore, the biosensor exhibited a fast response (<8 s), good anti-interference properties and high stability after relatively long-term storage (2 months). es
dc.description.sponsorship Ministerio de Economía y Competitividad IPT-2012-0961-300000, MAT2013-40852-R es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Elsevier es
dc.relation.ispartof Sensors and Actuators - B - Chemical Biochemical Sensors, 240, 37-45.
dc.relation.ispartof Sensors and Actuators - B - Chemical Biochemical Sensors, 240, 37-45.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Platinum es
dc.subject Magnetron sputtering es
dc.subject Magnetron sputtering es
dc.subject Polydopamine es
dc.subject Cholesterol es
dc.subject Glancing angle deposition es
dc.title Cholesterol biosensing with a polydopamine-modified nanostructured platinum electrode prepared by oblique angle physical vacuum deposition es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/submittedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.relation.projectID IPT-2012-0961-300000 es
dc.relation.projectID MAT2013-40852-R es
dc.relation.publisherversion http://dx.doi.org/10.1016/j.snb.2016.08.092 es
dc.identifier.doi 10.1016/j.snb.2016.08.092 es
idus.format.extent 38 p. es
dc.journaltitle Sensors and Actuators - B - Chemical Biochemical Sensors es
dc.publication.volumen 240 es
dc.publication.initialPage 37 es
dc.publication.endPage 45 es
dc.contributor.funder Ministerio de Economía y Competitividad (MINECO). España
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