dc.creator | Ozuna Serafini, Carmen Victoria | es |
dc.creator | Iehisa, Julio Cesar Masaru | es |
dc.creator | Giménez Alvear, María J. | es |
dc.creator | Álvarez, Juan B. | es |
dc.creator | Sousa Martín, Carolina | es |
dc.creator | Barro, Francisco | es |
dc.date.accessioned | 2018-11-05T15:19:12Z | |
dc.date.available | 2018-11-05T15:19:12Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Ozuna, C.V., Iehisa, J.C.M., Giménez Alvear, M.J., Álvarez, J.B., Sousa Martín, C. y Barro, F. (2015). Diversification of the celiac disease α-gliadin complex in wheat: A 33-mer peptide with six overlapping epitopes, evolved following polyploidization. Plant Journal, 82 (5), 794-805. | |
dc.identifier.issn | 0960-7412 | es |
dc.identifier.issn | 1365-313X | es |
dc.identifier.uri | https://hdl.handle.net/11441/79804 | |
dc.description.abstract | Summary The gluten proteins from wheat, barley and rye are responsible both for celiac disease (CD) and for non-celiac gluten sensitivity, two pathologies affecting up to 6-8% of the human population worldwide. The wheat α-gliadin proteins contain three major CD immunogenic peptides: p31-43, which induces the innate immune response; the 33-mer, formed by six overlapping copies of three highly stimulatory epitopes; and an additional DQ2.5-glia-α3 epitope which partially overlaps with the 33-mer. Next-generation sequencing (NGS) and Sanger sequencing of α-gliadin genes from diploid and polyploid wheat provided six types of α-gliadins (named 1-6) with strong differences in their frequencies in diploid and polyploid wheat, and in the presence and abundance of these CD immunogenic peptides. Immunogenic variants of the p31-43 peptide were found in most of the α-gliadins. Variants of the DQ2.5-glia-α3 epitope were associated with specific types of α-gliadins. Remarkably, only type 1 α-gliadins contained 33-mer epitopes. Moreover, the full immunodominant 33-mer fragment was only present in hexaploid wheat at low abundance, probably as the result of allohexaploidization events from subtype 1.2 α-gliadins found only in Aegilops tauschii, the D-genome donor of hexaploid wheat. Type 3 α-gliadins seem to be the ancestral type as they are found in most of the α-gliadin-expressing Triticeae species. These findings are important for reducing the incidence of CD by the breeding/selection of wheat varieties with low stimulatory capacity of T cells. Moreover, advanced genome-editing techniques (TALENs, CRISPR) will be easier to implement on the small group of α-gliadins containing only immunogenic peptides. Significance Statement The α-gliadins contain the most-immunogenic epitopes responsible of gluten intolerances. By NGS technology a comprehensive study of α-gliadins containing these immunogenic epitopes in diploid and polyploid wheats showed six types of α-gliadins. However, only Type 1, but not the others, contains all the immunogenic peptides and epitopes. These findings are important for preventing/reducing the incidence of CD and wheat breeding as we identify five types of α-gliadins that do not contain epitopes for the 33-mer. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad AGL2013-48946-C3-1-R | es |
dc.description.sponsorship | Junta de Andalucía P11-AGR-7920 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Wiley-Blackwell | es |
dc.relation.ispartof | Plant Journal, 82 (5), 794-805. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | 33-mer peptide | es |
dc.subject | Alpha-gliadin | es |
dc.subject | Celiac disease | es |
dc.subject | Wheat | es |
dc.title | Diversification of the celiac disease α-gliadin complex in wheat: A 33-mer peptide with six overlapping epitopes, evolved following polyploidization | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.projectID | AGL2013-48946-C3-1-R | es |
dc.relation.projectID | P11-AGR-7920 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1111/tpj.12851 | es |
dc.identifier.doi | 10.1111/tpj.12851 | es |
idus.format.extent | 12 p. | es |
dc.journaltitle | Plant Journal | es |
dc.publication.volumen | 82 | es |
dc.publication.issue | 5 | es |
dc.publication.initialPage | 794 | es |
dc.publication.endPage | 805 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | |
dc.contributor.funder | Junta de Andalucía | |