dc.creator | Bárány, Ivett | es |
dc.creator | Crespo González, José Luis | es |
dc.creator | Pérez Pérez, Yolanda | es |
dc.creator | Berenguer, Eduardo | es |
dc.creator | Risueño, M. Carmen | es |
dc.date.accessioned | 2018-06-15T09:55:12Z | |
dc.date.available | 2018-06-15T09:55:12Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Bárány, I., Crespo González, J.L., Pérez Pérez, Y., Berenguer, E. y Risueño, M.C. (2018). Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley. Journal of Experimental Biology, 69 (6), 1387-1402. | |
dc.identifier.issn | 1477-9145 | es |
dc.identifier.uri | https://hdl.handle.net/11441/76250 | |
dc.description.abstract | Microspores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role in autophagy by degrading autophagic material; less is known in plants. Plant cathepsins are papain-like C1A cysteine proteases involved in many physiological processes, including programmed cell death. We have analysed the involvement of autophagy in cell death, in relation to cathepsin activation, during stress-induced microspore embryogenesis in Hordeum vulgare. After stress, reactive oxygen species (ROS) and cell death increased and autophagy was activated, including HvATG5 and HvATG6 up-regulation and increase of ATG5, ATG8, and autophagosomes. Concomitantly, cathepsin L/F-, B-, and H-like activities were induced, cathepsin-like genes HvPap-1 and HvPap-6 were up-regulated, and HvPap-1, HvPap-6, and HvPap-19 proteins increased and localized in the cytoplasm, resembling autophagy structures. Inhibitors of autophagy and cysteine proteases reduced cell death and promoted embryogenesis. The findings reveal a role for autophagy in stress-induced cell death during microspore embryogenesis, and the participation of cathepsins. Similar patterns of activation, expression, and localization suggest a possible connection between cathepsins and autophagy. The results open up new possibilities to enhance microspore embryogenesis efficiency with autophagy and/or cysteine protease modulators. | es |
dc.description.sponsorship | España, MINECO AGL2014-52028-R and AGL2017-82447-R | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Company of Biologists | es |
dc.relation.ispartof | Journal of Experimental Biology, 69 (6), 1387-1402. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Autophagy | es |
dc.subject | Barley | es |
dc.subject | Caspase-like activity | es |
dc.subject | Bathepsins | es |
dc.subject | Cell death | es |
dc.subject | Cysteine | es |
dc.subject | C1A proteases | es |
dc.subject | Microspore embryogenesis | es |
dc.subject | ROS Stress response | es |
dc.title | Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | AGL2014-52028-R | es |
dc.relation.projectID | AGL2017-82447-R | es |
dc.relation.publisherversion | http://dx.doi.org/10.1093/jxb/erx455 | es |
dc.identifier.doi | 10.1093/jxb/erx455 | es |
idus.format.extent | 16 p. | es |
dc.journaltitle | Journal of Experimental Biology | es |
dc.publication.volumen | 69 | es |
dc.publication.issue | 6 | es |
dc.publication.initialPage | 1387 | es |
dc.publication.endPage | 1402 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | |