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In Vitro Comparative Study of Oxygen Plasma Treated Poly(Lactic–Co–Glycolic) (PLGA) Membranes and Supported Nanostructured Oxides for Guided Bone Regeneration Processes

 

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dc.creator Torres Lagares, Daniel es
dc.creator Castellanos Cosano, Lizett es
dc.creator Serrera Figallo, María de los Ángeles es
dc.creator López Santos, Carmen es
dc.creator Barranco Quero, Ángel es
dc.creator Rodríguez González-Elipe, Agustín es
dc.creator Gutiérrez Pérez, José Luis es
dc.date.accessioned 2018-06-07T10:24:27Z
dc.date.available 2018-06-07T10:24:27Z
dc.date.issued 2018
dc.identifier.citation Torres Lagares, D., Castellanos Cosano, L., Serrera Figallo, M.Á., López Santos, C., Barranco Quero, Á., Rodríguez González-Elipe, A. y Gutiérrez Pérez, J.L. (2018). In Vitro Comparative Study of Oxygen Plasma Treated Poly(Lactic–Co–Glycolic) (PLGA) Membranes and Supported Nanostructured Oxides for Guided Bone Regeneration Processes. Materials, 11 (5), 1-11.
dc.identifier.issn 1996-1944 es
dc.identifier.uri https://hdl.handle.net/11441/75811
dc.description.abstract Background: The use of physical barriers to prevent the invasion of gingival and connective tissue cells into bone cavities during the healing process is called guided bone regeneration. The objective of this in-vitro study was to compare the growth of human osteoblasts on Poly(Lactic–co–Glycolic) (PLGA) membranes modified with oxygen plasma and Hydroxyapatite (HA), silicon dioxide (SiO2), and titanium dioxide (TiO2) composite nanoparticles, respectively. (2) Methods: All the membranes received a common treatment with oxygen plasma and were subsequently treated with HA nanostructured coatings (n = 10), SiO2 (n = 10) and TiO2 (n = 10), respectively and a PLGA control membrane (n = 10). The assays were performed using the human osteoblast line MG-63 acquired from the Center for Scientific Instrumentation (CIC) from the University of Granada. The cell adhesion and the viability of the osteoblasts were analyzed by means of light-field microphotographs of each condition with the inverted microscope Axio Observer A1 (Carl Zeiss). For the determination of the mitochondrial energy balance, the MitoProbe™ JC-1 Assay Kit was employed. For the determination of cell growth and the morphology of adherent osteoblasts, two techniques were employed: staining with phalloidin-TRITC and staining with DAPI. (3) Results: The modified membranes that show osteoblasts with a morphology more similar to the control osteoblasts follow the order: PLGA/PO2/HA > PLGA/PO2/SiO2 > PLGA/PO2/TiO2 > PLGA (p < 0.05). When analysing the cell viability, a higher percentage of viable cells bound to the membranes was observed as follows: PLGA/PO2/SiO2 > PLGA/PO2/HA > PLGA/PO2/TiO2 > PLGA (p < 0.05), with a better energy balance of the cells adhered to the membranes PLGA/PO2/HA and PLGA/PO2/SiO2. (4) Conclusion: The membrane in which osteoblasts show characteristics more similar to the control osteoblasts is the PLGA/PO2/HA, followed by the PLGA/PO2/SiO2. es
dc.description.sponsorship España, Junta de Andalucía Consejería de Salud PI-0047-2013 es
dc.description.sponsorship Unión Europea, FEDER MAT2016- 79866-R es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher MDPI es
dc.relation.ispartof Materials, 11 (5), 1-11.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject guided bone regeneration es
dc.subject polylactic es
dc.subject membranes es
dc.subject PLGA es
dc.subject oxygen plasma es
dc.subject nanomedicine es
dc.title In Vitro Comparative Study of Oxygen Plasma Treated Poly(Lactic–Co–Glycolic) (PLGA) Membranes and Supported Nanostructured Oxides for Guided Bone Regeneration Processes es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Química orgánica es
dc.relation.projectID PI-0047-2013 es
dc.relation.projectID FEDER MAT2016- 79866-R es
dc.relation.publisherversion http://dx.doi.org/ 10.3390/ma11050752 es
dc.identifier.doi 10.3390/ma11050752 es
idus.format.extent 11 p. es
dc.journaltitle Materials es
dc.publication.volumen 11 es
dc.publication.issue 5 es
dc.publication.initialPage 1 es
dc.publication.endPage 11 es
dc.contributor.funder Junta de Andalucía
dc.contributor.funder European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
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