dc.creator | Vizcaíno González, Juan Antonio | es |
dc.creator | González, Francisco Javier | es |
dc.creator | Suárez, Mª Belén | es |
dc.creator | Redondo, José | es |
dc.creator | Heinrich, Julian | es |
dc.creator | Delgado Jarana, Jesús | es |
dc.creator | Hermosa, Rosa | es |
dc.creator | Gutiérrez, Santiago | es |
dc.creator | Monte, Enrique | es |
dc.creator | Llobell González, Antonio | es |
dc.creator | Rey, Manuel | es |
dc.date.accessioned | 2018-04-04T16:28:40Z | |
dc.date.available | 2018-04-04T16:28:40Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Vizcaíno González, J.A., González, F.J., Suárez, M.B., Redondo, J., Heinrich, J., Delgado Jarana, J.,...,Rey, M. (2006). Generation, annotation and analysis of ESTs from Trichoderma harzianum CECT 2413. BMC Genomics, 7, 1-14. | |
dc.identifier.issn | 1471-2164 (electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/71776 | |
dc.description.abstract | Background: The filamentous fungus Trichoderma harzianum is used as biological control agent of
several plant-pathogenic fungi. In order to study the genome of this fungus, a functional genomics
project called "TrichoEST" was developed to give insights into genes involved in biological control
activities using an approach based on the generation of expressed sequence tags (ESTs).
Results: Eight different cDNA libraries from T. harzianum strain CECT 2413 were constructed.
Different growth conditions involving mainly different nutrient conditions and/or stresses were
used. We here present the analysis of the 8,710 ESTs generated. A total of 3,478 unique sequences
were identified of which 81.4% had sequence similarity with GenBank entries, using the BLASTX
algorithm. Using the Gene Ontology hierarchy, we performed the annotation of 51.1% of the
unique sequences and compared its distribution among the gene libraries. Additionally, the
InterProScan algorithm was used in order to further characterize the sequences. The identification
of the putatively secreted proteins was also carried out. Later, based on the EST abundance, we
examined the highly expressed genes and a hydrophobin was identified as the gene expressed at
the highest level. We compared our collection of ESTs with the previous collections obtained from
Trichoderma species and we also compared our sequence set with different complete eukaryotic
genomes from several animals, plants and fungi. Accordingly, the presence of similar sequences in
different kingdoms was also studied.
Conclusion: This EST collection and its annotation provide a significant resource for basic and
applied research on T. harzianum, a fungus with a high biotechnological interest. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | BioMed Central | es |
dc.relation.ispartof | BMC Genomics, 7, 1-14. | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Generation, annotation and analysis of ESTs from Trichoderma harzianum CECT 2413 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.relation.publisherversion | http://dx.doi.org/ 10.1186/1471-2164-7-193 | es |
dc.identifier.doi | 10.1186/1471-2164-7-193 | es |
idus.format.extent | 14 p. | es |
dc.journaltitle | BMC Genomics | es |
dc.publication.volumen | 7 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 14 | es |