dc.creator | Cuecas Morano, María de Piedras Alba | es |
dc.creator | Cruces Tova, Jorge | es |
dc.creator | Galisteo López, Juan Francisco | es |
dc.creator | Peng, H. X. | es |
dc.creator | Gonzáles Grau, Juan Miguel | es |
dc.date.accessioned | 2018-04-04T10:09:03Z | |
dc.date.available | 2018-04-04T10:09:03Z | |
dc.date.issued | 2016-08-23 | |
dc.identifier.citation | Cuecas Morano, M.d.P.A., Cruces Tova, J., Galisteo López, J.F., Peng, H.X. y Gonzáles Grau, J.M. (2016). Cellular Viscosity in Prokaryotes and Thermal Stability of Low Molecular Weight Biomolecules. Biophysical Journal, 111 (4), 875-882. | |
dc.identifier.issn | 0006-3495 | es |
dc.identifier.uri | https://hdl.handle.net/11441/71708 | |
dc.description | Prepint, Texto y Gráficos | es |
dc.description.abstract | Some low molecular weight biomolecules, i.e., NAD(P)H, are unstable at high temperatures. The use of these biomolecules by thermophilic microorganisms has been scarcely analyzed. Herein, NADH stability has been studied at different temperatures and viscosities. NADH decay increased at increasing temperatures. At increasing viscosities, NADH decay rates decreased. Thus, maintaining relatively high cellular viscosity in cells could result in increased stability of low molecular weight biomolecules (i.e., NADH) at high temperatures, unlike what was previously deduced from studies in diluted water solutions. Cellular viscosity was determined using a fluorescent molecular rotor in various prokaryotes covering the range from 10 to 100°C. Some mesophiles showed the capability of changing cellular viscosity depending on growth temperature. Thermophiles and extreme thermophiles presented a relatively high cellular viscosity, suggesting this strategy as a reasonable mechanism to thrive under these high temperatures. Results substantiate the capability of thermophiles and extreme thermophiles (growth range 50–80°C) to stabilize and use generally considered unstable, universal low molecular weight biomolecules. In addition, this study represents a first report, to our knowledge, on cellular viscosity measurements in prokaryotes and it shows the dependency of prokaryotic cellular viscosity on species and growth temperature. | es |
dc.description.sponsorship | España Mineco CSD2009-0006 CGL2014-58762-P | es |
dc.description.sponsorship | Junta de Andalucía BIO-288 RNM2529 | es |
dc.description.sponsorship | NILS NILS 003-ABEL-CM-2013 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Biophysical Society | es |
dc.relation.ispartof | Biophysical Journal, 111 (4), 875-882. | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | low-molecular weight biomolecules | es |
dc.subject | thermostability | es |
dc.subject | cellular viscosity | es |
dc.subject | thermophiles | es |
dc.subject | fluorescent molecular rotor | es |
dc.subject | prokaryotes | es |
dc.title | Cellular Viscosity in Prokaryotes and Thermal Stability of Low Molecular Weight Biomolecules | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/submittedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.bpj.2016.07.024 | es |
dc.identifier.doi | 10.1016/j.bpj.2016.07.024 | es |
idus.format.extent | 8 p. | es |
dc.journaltitle | Biophysical Journal | es |
dc.publication.volumen | 111 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 875 | es |
dc.publication.endPage | 882 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | |
dc.contributor.funder | Junta de Andalucía | |