dc.creator | Elkhalfi, Bouchra | es |
dc.creator | Serrano Delgado, Aurelio | es |
dc.creator | Soukri, Abdelaziz | es |
dc.date.accessioned | 2018-01-22T10:06:24Z | |
dc.date.available | 2018-01-22T10:06:24Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Elkhalfi, B., Serrano Delgado, A. y Soukri, A. (2014). Identification of an extracellular infection-induced glyceraldehyde-3-phosphate dehydrogenase of the phytopathogenic proteobacterium Pseudomonas syringae pv tomato DC3000. Advances in Bioscience and Biotechnology, 5, 201-208. | |
dc.identifier.issn | 2156-8456 | es |
dc.identifier.uri | https://hdl.handle.net/11441/69286 | |
dc.description.abstract | According to molecular biology, genomic and proteo- mic data, the phytopathogenic gamma-proteobacte- rium Pseudomonas syringae pv. tomato DC3000 pro-duces a number of proteins that may promote infec- tion and draw nutrients from plants. Remarkably, P. syringae DC3000 strain possesses three paralogous gap genes encoding glyceraldehyde-3-phosphate dehy- drogenase (GAPDH) enzymes with different predic- ted molecular sizes and metabolic functions. As GAPDH was shown to be a virulence factor in other microbial pathogens, in the current study, we analyzed the ex-pression levels of each paralogous gap gene by real- time PCR to understand the actual impact of their protein products on P. syringae virulence. We found that all of them were strongly induced during the in-fection process. Nevertheless, proteomic analysis of cul- ture supernatants revealed that only Class I GAPDH1 encoded by the gap1 gene was identified as an extra-cellular protein in infective cells. These results strongly suggest that this GAPDH should play a role in the infective process, including its well-know en-zymatic function in the glycolytic metabolic pathway. | es |
dc.description.sponsorship | España AECID (MAEC) A1/043076/11 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Scientific Research Publishing | es |
dc.relation.ispartof | Advances in Bioscience and Biotechnology, 5, 201-208. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Infective State | es |
dc.subject | Gene Expression | es |
dc.subject | MALDI-TOF | es |
dc.subject | Bacterial Speak | es |
dc.subject | RNA | es |
dc.subject | Secretome | es |
dc.subject | GAPDH | es |
dc.title | Identification of an extracellular infection-induced glyceraldehyde-3-phosphate dehydrogenase of the phytopathogenic proteobacterium Pseudomonas syringae pv tomato DC3000 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | A1/043076/11 | es |
dc.relation.publisherversion | http://dx.doi.org/10.4236/abb.2014.53026 | es |
dc.identifier.doi | 10.4236/abb.2014.53026 | es |
idus.format.extent | 7 p. | es |
dc.journaltitle | Advances in Bioscience and Biotechnology | es |
dc.publication.volumen | 5 | es |
dc.publication.initialPage | 201 | es |
dc.publication.endPage | 208 | es |
dc.contributor.funder | Agencia Española de Cooperación Internacional para el Desarrollo (AECID) | |