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S-Sulfhydration: A Cysteine Posttranslational Modification in Plant Systems

Opened Access S-Sulfhydration: A Cysteine Posttranslational Modification in Plant Systems

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Autor: Aroca Aguilar, Ángeles
Serna, Antonio
Gotor Martínez, Cecilia
Romero González, Luis Carlos
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2015
Tipo de documento: Artículo
Resumen: Hydrogen sulfide is a highly reactive molecule that is currently accepted as a signaling compound. This molecule is as important as carbon monoxide in mammals and hydrogen peroxide in plants, as well as nitric oxide in both eukaryotic systems. Although many studies have been conducted on the physiological effects of hydrogen sulfide, the underlying mechanisms are poorly understood. One of the proposed mechanisms involves the posttranslational modification of protein cysteine residues, a process called S-sulfhydration. In this work, a modified biotin switch method was used for the detection of Arabidopsis (Arabidopsis thaliana) proteins modified by S-sulfhydration under physiological conditions. The presence of an S-sulfhydration-modified cysteine residue on cytosolic ascorbate peroxidase was demonstrated using liquid chromatography-tandem mass spectrometry analysis, and a total of 106 S-sulfhydrated proteins were identified. Immunoblot and enzyme activity analyses of some of these pro...
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Tamaño: 2.064Mb
Formato: PDF

URI: https://hdl.handle.net/11441/69140

DOI: 10.1104/pp.15.00009

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