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Thioredoxin f1 and NADPH-dependent thioredoxin reductase C have overlapping functions in regulating photosynthetic metabolism and plant growth in response to varying light conditions

 

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dc.creator Thormählen, Ina es
dc.creator Meitzel, Tobias es
dc.creator Groysman, Julia es
dc.creator Naranjo Río-Miranda, Belén es
dc.creator Cejudo Fernández, Francisco Javier es
dc.date.accessioned 2018-01-11T12:02:09Z
dc.date.available 2018-01-11T12:02:09Z
dc.date.issued 2016
dc.identifier.citation Thormählen, I., Meitzel, T., Groysman, J., Naranjo Rio-Miranda, B. y Cejudo Fernández, F.J. (2016). Thioredoxin f1 and NADPH-dependent thioredoxin reductase C have overlapping functions in regulating photosynthetic metabolism and plant growth in response to varying light conditions. Plant Physiology, 169 (3), 1766-1786.
dc.identifier.issn 0032-0889 es
dc.identifier.uri http://hdl.handle.net/11441/68771
dc.description.abstract Two different thiol-redox-systems exist in plant chloroplasts, the ferredoxin-thioredoxin system, which depends of ferredoxin reduced by the photosynthetic electron-transport chain and, thus, of light, and the NADPH-dependent thioredoxin reductase C (NTRC) system, which relies on NADPH and thus may be linked to sugar metabolism in the dark. Previous studies suggested therefore that the two different systems may have different functions in plants. We now report that there is a previously unrecognized functional redundancy of thioredoxin-f1 and NTRC in regulating photosynthetic metabolism and growth. In Arabidopsis mutants, combined - but not single - deficiencies of thioredoxin-f1 and NTRC led to severe growth inhibition and perturbed light acclimation, accompanied by strong impairments of Calvin-Benson-cycle activity and starch accumulation. Light-activation of key-enzymes of these pathways, fructose-1,6-bisphosphatase and ADP-glucose pyrophosphorylase, was almost completely abolished. The subsequent increase in NADPH/NADP+ and ATP/ADP ratios led to increased nitrogen assimilation, NADP-malate dehydrogenase activation and light-vulnerability of photosystem I core-proteins. In an additional approach, reporter studies show that Trx f1 and NTRC proteins are both co-localized in the same chloroplast substructure. Results provide genetic evidence that light and NADPH dependent thiol-redox systems interact at the level of thioredoxin-f1 and NTRC to coordinately participate in the regulation of Calvin-Benson-cycle, starch metabolism and growth in response to varying light conditions. es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher American Society of Plant Biologists es
dc.relation.ispartof Plant Physiology, 169 (3), 1766-1786.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title Thioredoxin f1 and NADPH-dependent thioredoxin reductase C have overlapping functions in regulating photosynthetic metabolism and plant growth in response to varying light conditions es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/submittedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular es
dc.relation.publisherversion http://dx.doi.org/10.1104/pp.15.01122 es
dc.identifier.doi 10.1104/pp.15.01122 es
idus.format.extent 20 es
dc.journaltitle Plant Physiology es
dc.publication.volumen 169 es
dc.publication.issue 3 es
dc.publication.initialPage 1766 es
dc.publication.endPage 1786 es
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