dc.creator | Vega Vaquero, Alejandro | es |
dc.creator | Bonoro, Giancarlo | es |
dc.creator | Morselli, Marco | es |
dc.creator | Vaquero Sedas, María Isabel | es |
dc.creator | Rubbi, Liudmilla | es |
dc.creator | Pellegrini, M. | es |
dc.creator | Vega Palas, Miguel Ángel | es |
dc.date.accessioned | 2018-01-09T18:13:05Z | |
dc.date.available | 2018-01-09T18:13:05Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Vega Vaquero, A., Bonoro, G., Morselli, M., Vaquero Sedas, M.I., Rubbi, L., Pellegrini, M. y Vega Palas, M.Á. (2016). Novel features of telomere biology revealed by the absence of telomeric DNA methylation. Genome Research, 26, 1047-1056. | |
dc.identifier.issn | 1088-9051 | es |
dc.identifier.uri | http://hdl.handle.net/11441/68550 | |
dc.description.abstract | Cytosine methylation regulates the length and stability of telomeres, which can affect a wide variety of biological features,
including cell differentiation, development, or illness. Although it is well established that subtelomeric regions are methylated,
the presence of methylated cytosines at telomeres has remained controversial. Here, we have analyzed multiple bisulfite
sequencing studies to address the methylation status of Arabidopsis thaliana telomeres. We found that the levels of
estimated telomeric DNA methylation varied among studies. Interestingly, we estimated higher levels of telomeric DNA
methylation in studies that produced C-rich telomeric strands with lower efficiency. However, these high methylation estimates
arose due to experimental limitations of the bisulfite technique. We found a similar phenomenon for mitochondrial
DNA: The levels of mitochondrial DNA methylation detected were higher in experiments with lower mitochondrial read
production efficiencies. Based on experiments with high telomeric C-rich strand production efficiencies, we concluded that
Arabidopsis telomeres are not methylated, which was confirmed by methylation-dependent restriction enzyme analyses.
Thus, our studies indicate that telomeres are refractory to de novo DNA methylation by the RNA-directed DNA methylation
machinery. This result, together with previously reported data, reveals that subtelomeric DNA methylation controls
the homeostasis of telomere length | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Cold Spring Harbor Laboratory Press | es |
dc.relation.ispartof | Genome Research, 26, 1047-1056. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Novel features of telomere biology revealed by the absence of telomeric DNA methylation | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.publisherversion | http://dx.doi.org/10.1101/gr.202465.115 | es |
dc.identifier.doi | 10.1101/gr.202465.115 | es |
idus.format.extent | 10 p. | es |
dc.journaltitle | Genome Research | es |
dc.publication.volumen | 26 | es |
dc.publication.initialPage | 1047 | es |
dc.publication.endPage | 1056 | es |