dc.creator | Bornikoel, Jan | es |
dc.creator | Carrión, Alejandro | es |
dc.creator | Fan, Qing | es |
dc.creator | Flores García, Enrique | es |
dc.creator | Forchhammer, Karl | es |
dc.creator | Mariscal, Vicente | es |
dc.date.accessioned | 2017-11-28T15:45:47Z | |
dc.date.available | 2017-11-28T15:45:47Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Bornikoel, J., Carrión, A., Fan, Q., Flores, E., Forchhammer, K. y Mariscal, V. (2017). Role of Two Cell Wall Amidases in Septal Junction and Nanopore Formation in the Multicellular Cyanobacterium Anabaena sp. PCC 7120. Frontiers in Cellular and Infection Microbiology, 7 (386), 1-15. | |
dc.identifier.issn | 2235-2988 | es |
dc.identifier.uri | http://hdl.handle.net/11441/66864 | |
dc.description.abstract | Filamentous cyanobacteria have developed a strategy to perform incompatible
processes in one filament by differentiating specialized cell types, N2-fixing heterocysts
and CO2-fixing, photosynthetic, vegetative cells. These bacteria can be considered
true multicellular organisms with cells exchanging metabolites and signaling molecules
via septal junctions, involving the SepJ and FraCD proteins. Previously, it was shown
that the cell wall lytic N-acetylmuramyl-L-alanine amidase, AmiC2, is essential for
cell–cell communication in Nostoc punctiforme. This enzyme perforates the septal
peptidoglycan creating an array of nanopores, which may be the framework for septal
junction complexes. In Anabaena sp. PCC 7120, two homologs of AmiC2, encoded
by amiC1 and amiC2, were identified and investigated in two different studies. Here,
we compare the function of both AmiC proteins by characterizing different Anabaena
amiC mutants, which was not possible in N. punctiforme, because there the amiC1
gene could not be inactivated. This study shows the different impact of each protein
on nanopore array formation, the process of cell–cell communication, septal protein
localization, and heterocyst differentiation. Inactivation of either amidase resulted in
significant reduction in nanopore count and in the rate of fluorescent tracer exchange
between neighboring cells measured by FRAP analysis. In an amiC1 amiC2 double
mutant, filament morphology was affected and heterocyst differentiation was abolished.
Furthermore, the inactivation of amiC1 influenced SepJ localization and prevented the
filament-fragmentation phenotype that is characteristic of sepJ or fraC fraD mutants.
Our findings suggest that both amidases are to some extent redundant in their function,
and describe a functional relationship of AmiC1 and septal proteins SepJ and FraCD | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Media | es |
dc.relation.ispartof | Frontiers in Cellular and Infection Microbiology, 7 (386), 1-15. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cyanobacteria | es |
dc.subject | Heterocysts | es |
dc.subject | Peptidoglycan | es |
dc.subject | Amidase | es |
dc.subject | AmiC | es |
dc.subject | Septal junctions | es |
dc.subject | Cell-cell communication | es |
dc.subject | SepJ | es |
dc.title | Role of Two Cell Wall Amidases in Septal Junction and Nanopore Formation in the Multicellular Cyanobacterium Anabaena sp. PCC 7120 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.publisherversion | http://dx.doi.org/10.3389/fcimb.2017.00386 | es |
dc.identifier.doi | 10.3389/fcimb.2017.00386 | es |
idus.format.extent | 15 p. | es |
dc.journaltitle | Frontiers in Cellular and Infection Microbiology | es |
dc.publication.volumen | 7 | es |
dc.publication.issue | 386 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 15 | es |