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Interaction of photosystem I from Phaeodactylum tricornutum with plastocyanins as compared with its native cytochrome c6: Reunion with a lost donor

 

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dc.creator Bernal Bayard, Pilar es
dc.creator Pallara, Chiara es
dc.creator Castell, M. Carmen es
dc.creator Molina Heredia, Fernando Publio es
dc.creator Fernández Recio, Juan es
dc.creator Hervás Morón, Manuel es
dc.creator Navarro Carruesco, José Antonio es
dc.date.accessioned 2017-11-15T16:18:56Z
dc.date.available 2017-11-15T16:18:56Z
dc.date.issued 2015
dc.identifier.citation Bernal Bayard, P., Pallara, C., Castell, M.C., Molina Heredia, F.P., Fernández Recio, J., Hervás Morón, M. y Navarro Carruesco, J.A. (2015). Interaction of photosystem I from Phaeodactylum tricornutum with plastocyanins as compared with its native cytochrome c6: Reunion with a lost donor. Biochimica et Biophysica Acta - Bioenergetics, 1847 (12), 1549-1559.
dc.identifier.uri http://hdl.handle.net/11441/66085
dc.description.abstract In the Phaeodactylum tricornutum alga, as in most diatoms, cytochrome c6 is the only electron donor to photosystem I, and thus they lack plastocyanin as an alternative electron carrier. We have investigated, by using laser-flash absorption spectroscopy, the electron transfer to Phaeodactylum photosystem I from plastocyanins from cyanobacteria, green algae and plants, as compared with its own cytochrome c6. Diatom photosystem I is able to effectively react with eukaryotic acidic plastocyanins, although with less efficiency than with Phaeodactylum cytochrome c6. This efficiency, however, increases in some green alga plastocyanin mutants mimicking the electrostatics of the interaction site on the diatom cytochrome. In addition, the structure of the transient electron transfer complex between cytochrome c6 and photosystem I from Phaeodactylum has been analyzed by computational docking and compared to that of green lineage and mixed systems. Taking together, the results explain why the Phaeodactylum system shows a lower efficiency than the green systems, both in the formation of the properly arranged [cytochrome c6-photosystem I] complex and in the electron transfer itself. es
dc.description.sponsorship Ministerio de Economía y Competitividad BIO2012-35271, BIO2013-48213-R es
dc.description.sponsorship Junta de Andalucía BIO-022 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Elsevier es
dc.relation.ispartof Biochimica et Biophysica Acta - Bioenergetics, 1847 (12), 1549-1559.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Cytochrome c6 es
dc.subject Electron transfer es
dc.subject Computational docking es
dc.subject Laser flash photolysis es
dc.subject Phaeodactylum es
dc.subject Photosystem I es
dc.subject Plastocyanin es
dc.title Interaction of photosystem I from Phaeodactylum tricornutum with plastocyanins as compared with its native cytochrome c6: Reunion with a lost donor es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/acceptedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.relation.projectID BIO2012-35271 es
dc.relation.projectID BIO2013-48213-R es
dc.relation.projectID BIO-022 es
dc.relation.publisherversion http://dx.doi.org/10.1016/j.bbabio.2015.09.006 es
dc.identifier.doi 10.1016/j.bbabio.2015.09.006 es
idus.format.extent 42 p. es
dc.journaltitle Biochimica et Biophysica Acta - Bioenergetics es
dc.publication.volumen 1847 es
dc.publication.issue 12 es
dc.publication.initialPage 1549 es
dc.publication.endPage 1559 es
dc.contributor.funder Ministerio de Economía y Competitividad (MINECO). España
dc.contributor.funder Junta de Andalucía
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