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Development and validation of an RP-HPLC method for CB13 evaluation in several PLGA nanoparticle systems

Opened Access Development and validation of an RP-HPLC method for CB13 evaluation in several PLGA nanoparticle systems

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Autor: Álvarez Fuentes, Josefa
Martín Banderas, Lucía
Muñoz Rubio, Inmaculada
Holgado Villafuerte, María Ángeles
Fernández Arévalo, María Mercedes
Departamento: Universidad de Sevilla. Departamento de Farmacia y Tecnología Farmacéutica
Fecha: 2012
Publicado en: The Scientific World Journal, 2012, 737526-.
Tipo de documento: Artículo
Resumen: simple, fast, and reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for determining of a cannabinoid derivate, which displays potent antihyperalgesic activity, 1-naphthalenyl[4-(pentyloxy)-1-naphthalenyl]methanone (CB13) into PLGA nanoparticles. Separation was achieved in a C18 column using a mobile phase consisting of two solvents: solvent A, consisting of acetonitrile:water:acetic acid (75:23.7:1.3v/v), and solvent B, consisting of acetonitrile. An isocratic method (70:30v/v), with a flow rate of 1.000mL/min, and a diode array detector were used. The developed method was precise, accurate, and linear over the concentration range of analysis with a limit of detection and a limit of quantification of 0.5 and 1.25 μg/mL, respectively. The developed method was applied to the analysis of CB13 in nanoparticles samples obtained by three different procedures (SEV, FF, and NPP) in terms of encapsulation efficiency and drug release. Nanop...
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Cita: Álvarez Fuentes, J., Martín Banderas, L., Muñoz Rubio, I., Holgado Villafuerte, M.Á. y Fernández Arévalo, M.M. (2012). Development and validation of an RP-HPLC method for CB13 evaluation in several PLGA nanoparticle systems. The Scientific World Journal, 2012, 737526-.
Tamaño: 1.901Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64459

DOI: 10.1100/2012/737526

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