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Disruption of both chloroplastic and cytosolic FBPase genes results in a dwarf phenotype and important starch and metabolite changes in Arabidopsis thaliana

Opened Access Disruption of both chloroplastic and cytosolic FBPase genes results in a dwarf phenotype and important starch and metabolite changes in Arabidopsis thaliana

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Autor: Rojas González, José A.
Soto Súarez, Mauricio
García Díaz, Ángel
Romero Puertas, María C.
Sandalio, Luisa M.
Mérida Berlanga, Ángel
Thormählen, Ina
Serrato, Antonio J.
Sahrawy, Mariam
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2015
Publicado en: Journal of Experimental Botany, 66 (9), 2673-2689.
Tipo de documento: Artículo
Resumen: In this study, evidence is provided for the role of fructose-1,6-bisphosphatases (FBPases) in plant development and carbohydrate synthesis and distribution by analysing two Arabidopsis thaliana T-DNA knockout mutant lines, cyfbp and cfbp1, and one double mutant cyfbp cfbp1 which affect each FBPase isoform, cytosolic and chloroplastic, respectively. cyFBP is involved in sucrose synthesis, whilst cFBP1 is a key enzyme in the Calvin–Benson cycle. In addition to the smaller rosette size and lower rate of photosynthesis, the lack of cFBP1 in the mutants cfbp1 and cyfbp cfbp1 leads to a lower content of soluble sugars, less starch accumulation, and a greater superoxide dismutase (SOD) activity. The mutants also had some developmental alterations, including stomatal opening defects and increased numbers of root vascular layers. Complementation also confirmed that the mutant phenotypes were caused by disruption of the cFBP1 gene. cyfbp mutant plants without cyFBP showed a higher starch conten...
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Cita: Rojas González, J.A., Soto Súarez, M., García Díaz, Á., Romero Puertas, M.C., Sandalio, .M., Mérida Berlanga, Á.,...,Sahrawy, M. (2015). Disruption of both chloroplastic and cytosolic FBPase genes results in a dwarf phenotype and important starch and metabolite changes in Arabidopsis thaliana. Journal of Experimental Botany, 66 (9), 2673-2689.
Tamaño: 4.249Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64169

DOI: 10.1093/jxb/erv062

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