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Determination of Arabidopsis thaliana telomere length by PCR

Opened Access Determination of Arabidopsis thaliana telomere length by PCR

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Autor: Vaquero Sedas, María Isabel
Vega Palas, Miguel Ángel
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2014
Publicado en: Scientific Reports, 4 (5540), 1-7.
Tipo de documento: Artículo
Resumen: In humans, telomere length studies have acquired great relevance because the length of telomeres has been related to natural processes like disease, aging and cancer. However, very little is known about the influence of telomere length on the biology of wild type plants. The length of plant telomeres has been usually studied by Terminal Restriction Fragment (TRF) analyses. This technique requires high amounts of tissue, including multiple cell types, which might be the reason why very little is known about the influence of telomere length on plant natural processes. In contrast, many of the human telomere length studies have focused on homogenous cell populations. Most of these studies have been performed by PCR, using telomeric degenerated primers, which allow the determination of telomere length from small amounts of human cells. Here, we have adapted the human PCR procedure to analyze the length of Arabidopsis thaliana telomeres. This PCR approach will facilitate the analysis of te...
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Cita: Vaquero Sedas, M.I. y Vega Palas, M.Á. (2014). Determination of Arabidopsis thaliana telomere length by PCR. Scientific Reports, 4 (5540), 1-7.
Tamaño: 1.440Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64061

DOI: 10.1038/srep05540

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